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陆地棉非整倍体的诱发和鉴定
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作者 wang-zhen guo 《棉花学报》 CSCD 北大核心 2002年第z1期97-,共1页
  Aneuploid lines have been playing an important role in crop genetic studies and breeding utilization. Unfortunately, only several genetic materials of aneuploid can be used in Upland cotton. So far, there are 16 m...   Aneuploid lines have been playing an important role in crop genetic studies and breeding utilization. Unfortunately, only several genetic materials of aneuploid can be used in Upland cotton. So far, there are 16 monosomics identified in cotton. In order to fill the gap, we carried on the irradiation research of mature pollen grains with γ-rays at different dosages and further produced F1 generation.   …… 展开更多
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Quantitative Trait Loci Mapping of Leaf Morphological Traits and Chlorophyll Content in Cultivated Tetraploid Cotton 被引量:13
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作者 Xian-Liang SONG wang-zhen guo +1 位作者 Zhi-guo HAN Tian-Zhen ZHANG 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2005年第11期1382-1390,共9页
Genetic mapping provides a powerful tool for quantitative trait loci (QTL) analysis at the molecular level. A simple sequence repeat (SSR) genetic map containing 590 markers and a BCI population from two cultivate... Genetic mapping provides a powerful tool for quantitative trait loci (QTL) analysis at the molecular level. A simple sequence repeat (SSR) genetic map containing 590 markers and a BCI population from two cultivated tetraploid cotton (Gossypium hirsutum L.) cultivars, namely TM-1 and Hai 7124 (G. barbadense L.), were used to map and analyze QTL using the composite interval mapping (CIM) method. Thirty one QTLs, 10 for lobe length, 13 for lobe width, six for lobe angle, and two for leaf chlorophyll content, were detected on 15 chromosomes or linkage groups at logarithm of odds (LOD)≥2.0, of which 15 were found for leaf morphology at LOD≥3.0. The genetic effects of the QTL were estimated. These results are fundamental for marker-assisted selection (MAS) of these traits in tetraploid cotton breeding. 展开更多
关键词 CHLOROPHYLL COTTON leaf morphology quantitative trait locus mapping simple sequencerepeat.
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Cloning and Expression of Low Molecular Weight Glutenin Genes from the Chinese Elite Wheat Cultivar "Xiaoyan 54" 被引量:4
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作者 Xin-Yu Wang Kun-Fan Liu wang-zhen guo 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2006年第2期212-218,共7页
The low molecular weight (LMW) glutenln subunlts account for 40% of wheat gluten protein content by mass and these proteins are considered to significantly affect dough quality characteristics. Five new full-length ... The low molecular weight (LMW) glutenln subunlts account for 40% of wheat gluten protein content by mass and these proteins are considered to significantly affect dough quality characteristics. Five new full-length LMW glutenln genes (designated LMW-5, LMW-7, LMW-42, LMW-58, and LMW-34) were isolated from the Chinese elite wheat cultivar "Xlaoyan 54" by PCR amplification of genomlc DNA using a pair of degenerate primers designed from the conserved sequences of the N- and C-terminal regions of published LMW glutenln genes. Deduced amino acid sequence analysis showed that LMW-5 belongs to the LMW-i type genes and that the other four belong to LMW-m type genes. Sequence comparisons revealed that point mutations occasionally occurred in signal peptide and N-terminus domains and often existed in domain III and domain V. Small insertions and deletions are represented in the repetitive domain. There is a stop codon after amino acid position 110 In the repetitive domain of LMW.34, indicating that It is a pseudogene. The other four genes have complete open reading frames and the putative mature regions of these genes were subcloned Into pET-30a expression vector and successfully expressed in Escherlchla coll. Protein sodium dodecyl sulfate-polyacrylamlde gel electro- phoresls analysis showed that all proteins expressed in E. coil by the four genes could be related to B-group LMW glutenln subunits of wheat. 展开更多
关键词 low molecular weight glutenin subunit M-type low molecular weight glutenin gene I-type low molecular weightglutenin gene.
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