The colony-forming Phaeocystis species are causative agents of dense bloom occurrences incoastal waters worldwide. It is difficult to separate them because of the different morphologies associated with their colonial ...The colony-forming Phaeocystis species are causative agents of dense bloom occurrences incoastal waters worldwide. It is difficult to separate them because of the different morphologies associated with their colonial stages. In this study we applied molecular approaches to analyze the genetic variation of Phaeocystis globosa and Phaeocystis pouchetii from several geographic regions, and to assist in tracing the dispersal of bloom-forming Phaeocystis species in coastal waters of China. The sequences of the internal transcribed spacers (ITS1 and ITS2) of rDNA and the 5. 8S ribosomal RNA gene of Phaeocystis strains were determined. Sequence comparison shows that P.globosa was the most divergent to P. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences existed between strains of P. globosa. The sequence comparison of the Phaeocystis rDNA ITS clearly shows that the species isolated from the southeast coast of China is identified as P. globosa rather than P. cf. pouchetii or other species. Furthermore, the significance of rDNA variation in distinct global populations of P. globosa suggested it might have had sufficient time to accumulate detectable mutations at the rDNA locus, supporting the hypothesis of ancient dispersal of P .globosa to many areas, meaning that P. globosa blooms in the coastal waters of China are endemic rather than a newly introduced species or a foreign source. Finally, based on the high divergent region of rDNA ITS, a pair of species-specific primers for P. globosa were designed, they could be useful to detect the presence of this species in mixed plankton assemblages or flagellate stages that are recognized with diffculties by means of conventional microscopy.展开更多
Data collection, factor composition, nappe analysis and integrative simulation of natural geographical factors in Erlong Lake watershed have been carried out based on GIS. The risk areas where non-point source polluti...Data collection, factor composition, nappe analysis and integrative simulation of natural geographical factors in Erlong Lake watershed have been carried out based on GIS. The risk areas where non-point source pollution may occur were compartmentalized and assessed, and the total soil erosion and the runoffs of N and P with rainfall in this valley were worked out by experiment and GIS mapping. The study indicated that the main type of soil erosion was moderate (erosion modulus is 1000-2500t/(km(2).a)) at present, and the intense erosion areas are located in dry land with variable slope east of the lake and the middle-south parts of steep slope mountainous region (erosion modulus is more than 5000t/(km(2).a)). Though the area is small, it should be paid attention to. The trend of non-point source pollution (NSP) of nitrogen and phosphorus loss was corresponded with the soil erosion. Spatial distribution and the reasons of the distribution difference have been presented and it was emphasized that the human activities among the influence factors was the most important. It surely offers a scientific basis to control and prevent non-point source pollution in the watershed.展开更多
Under the condition of different precipitation intensities, different gradients, different land-use types and different vegetation coverage, the soil erosion and transference of element (or pollutant) are studied by s...Under the condition of different precipitation intensities, different gradients, different land-use types and different vegetation coverage, the soil erosion and transference of element (or pollutant) are studied by simulating and analyzing the surface run-off of experimental plots in the catchment area of Songhua Lake, with an area of about 43 370.8km2. And the influencing factors that produce the spatial difference are analyzed and assessed. It is put forward that the irrational land utilization is the reason of soil erosion and pollutant run-off. The gradient of farmland, the growing season of vegetation and the vegetation coverage are chiefly restricting factors that lead to the soil erosion and pollutant run-off. This study can provide the fundamental data for comprehensive planning and harnessing of the non-point source pollution in the valley.展开更多
From the point of view of the interplay between order and chaos, the most regular single-particle motion of neutrons has been found in the superheavy system with and based on the Skyrme–Hartree–Fock model and in t...From the point of view of the interplay between order and chaos, the most regular single-particle motion of neutrons has been found in the superheavy system with and based on the Skyrme–Hartree–Fock model and in the system with and based on the relativistic mean-field model. It has been shown that the statistical analysis of spectra can give valuable information about the stability of suprheavy systems. In addition it may yield deep insight into the single-particle motion in the mean field formed by the superheavy system.展开更多
A shallow water tomography scheme based on the modal wave number inversion technique is considered in this paper. The scheme is based on the assumption that modal wave number for trapped modes can be measured in a sui...A shallow water tomography scheme based on the modal wave number inversion technique is considered in this paper. The scheme is based on the assumption that modal wave number for trapped modes can be measured in a suitable way. The tomographic inversion is accomplished in two steps: firstly, the bottom parameters are inverted by using the bottom reflection phase shift with the known sound speed profile; secondly, the variation of sound speed profile at different time is inverted provided the bottom parameters are known. A numerical simulation shows that the proposed scheme works well, and the sensitivity analysis of sound speed profile inversion is performed for shallow water environmental parameters: sound speed, density and attenuation coefficient of the bottom.展开更多
基金This study was supported by the National Natural Science Foundation of China under contract No. 39970063the Natural Science Foundation of Guangdong Province, China under contract No. 001213the Red-tide Key Project of the Natural Science Foundatio
文摘The colony-forming Phaeocystis species are causative agents of dense bloom occurrences incoastal waters worldwide. It is difficult to separate them because of the different morphologies associated with their colonial stages. In this study we applied molecular approaches to analyze the genetic variation of Phaeocystis globosa and Phaeocystis pouchetii from several geographic regions, and to assist in tracing the dispersal of bloom-forming Phaeocystis species in coastal waters of China. The sequences of the internal transcribed spacers (ITS1 and ITS2) of rDNA and the 5. 8S ribosomal RNA gene of Phaeocystis strains were determined. Sequence comparison shows that P.globosa was the most divergent to P. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences existed between strains of P. globosa. The sequence comparison of the Phaeocystis rDNA ITS clearly shows that the species isolated from the southeast coast of China is identified as P. globosa rather than P. cf. pouchetii or other species. Furthermore, the significance of rDNA variation in distinct global populations of P. globosa suggested it might have had sufficient time to accumulate detectable mutations at the rDNA locus, supporting the hypothesis of ancient dispersal of P .globosa to many areas, meaning that P. globosa blooms in the coastal waters of China are endemic rather than a newly introduced species or a foreign source. Finally, based on the high divergent region of rDNA ITS, a pair of species-specific primers for P. globosa were designed, they could be useful to detect the presence of this species in mixed plankton assemblages or flagellate stages that are recognized with diffculties by means of conventional microscopy.
文摘Data collection, factor composition, nappe analysis and integrative simulation of natural geographical factors in Erlong Lake watershed have been carried out based on GIS. The risk areas where non-point source pollution may occur were compartmentalized and assessed, and the total soil erosion and the runoffs of N and P with rainfall in this valley were worked out by experiment and GIS mapping. The study indicated that the main type of soil erosion was moderate (erosion modulus is 1000-2500t/(km(2).a)) at present, and the intense erosion areas are located in dry land with variable slope east of the lake and the middle-south parts of steep slope mountainous region (erosion modulus is more than 5000t/(km(2).a)). Though the area is small, it should be paid attention to. The trend of non-point source pollution (NSP) of nitrogen and phosphorus loss was corresponded with the soil erosion. Spatial distribution and the reasons of the distribution difference have been presented and it was emphasized that the human activities among the influence factors was the most important. It surely offers a scientific basis to control and prevent non-point source pollution in the watershed.
文摘Under the condition of different precipitation intensities, different gradients, different land-use types and different vegetation coverage, the soil erosion and transference of element (or pollutant) are studied by simulating and analyzing the surface run-off of experimental plots in the catchment area of Songhua Lake, with an area of about 43 370.8km2. And the influencing factors that produce the spatial difference are analyzed and assessed. It is put forward that the irrational land utilization is the reason of soil erosion and pollutant run-off. The gradient of farmland, the growing season of vegetation and the vegetation coverage are chiefly restricting factors that lead to the soil erosion and pollutant run-off. This study can provide the fundamental data for comprehensive planning and harnessing of the non-point source pollution in the valley.
文摘From the point of view of the interplay between order and chaos, the most regular single-particle motion of neutrons has been found in the superheavy system with and based on the Skyrme–Hartree–Fock model and in the system with and based on the relativistic mean-field model. It has been shown that the statistical analysis of spectra can give valuable information about the stability of suprheavy systems. In addition it may yield deep insight into the single-particle motion in the mean field formed by the superheavy system.
文摘A shallow water tomography scheme based on the modal wave number inversion technique is considered in this paper. The scheme is based on the assumption that modal wave number for trapped modes can be measured in a suitable way. The tomographic inversion is accomplished in two steps: firstly, the bottom parameters are inverted by using the bottom reflection phase shift with the known sound speed profile; secondly, the variation of sound speed profile at different time is inverted provided the bottom parameters are known. A numerical simulation shows that the proposed scheme works well, and the sensitivity analysis of sound speed profile inversion is performed for shallow water environmental parameters: sound speed, density and attenuation coefficient of the bottom.
