Colorectal cancer(CRC)is a complex and heterogeneous disease that is the second-leading cause of cancer-related deaths worldwide.Mesenchymal colorectal cancer(m CRC)accounts for about one-third of all CRC.Despite sign...Colorectal cancer(CRC)is a complex and heterogeneous disease that is the second-leading cause of cancer-related deaths worldwide.Mesenchymal colorectal cancer(m CRC)accounts for about one-third of all CRC.Despite significant advances in understanding the molecular mechanisms underlying m CRC,effective treatments for patients with advanced disease remain limited,particularly for the aggressive mesenchymal subtype of CRC(Rawla et al.,2019).展开更多
Reverse genetic screens are invaluable for uncovering gene functions, but are traditionally hampered by some technical limitations. Over the past few years, since the advent of the revolutionary CRISPR/Cas9 technology...Reverse genetic screens are invaluable for uncovering gene functions, but are traditionally hampered by some technical limitations. Over the past few years, since the advent of the revolutionary CRISPR/Cas9 technology, its power in genome editing has been harnessed to overcome the traditional limitations in reverse genetic screens, with successes in various biological contexts. Here, we outline these CRISPR/Cas9-based screens, provide guidance on the design of effective screens and discuss the potential future directions of development of this field.展开更多
文摘Colorectal cancer(CRC)is a complex and heterogeneous disease that is the second-leading cause of cancer-related deaths worldwide.Mesenchymal colorectal cancer(m CRC)accounts for about one-third of all CRC.Despite significant advances in understanding the molecular mechanisms underlying m CRC,effective treatments for patients with advanced disease remain limited,particularly for the aggressive mesenchymal subtype of CRC(Rawla et al.,2019).
基金supported by the grant from the National Natural Science Foundation of China(No. 31670919 to H.W.)the 1000-Youth Elite Program of China to H.W
文摘Reverse genetic screens are invaluable for uncovering gene functions, but are traditionally hampered by some technical limitations. Over the past few years, since the advent of the revolutionary CRISPR/Cas9 technology, its power in genome editing has been harnessed to overcome the traditional limitations in reverse genetic screens, with successes in various biological contexts. Here, we outline these CRISPR/Cas9-based screens, provide guidance on the design of effective screens and discuss the potential future directions of development of this field.
