Propofol,a common intravenous anesthetic used in clinical,has been shown to regulate cells proliferation,inflammation,angiogenesis and metastasis as well as exerting anti-cancer effect in several different types of ca...Propofol,a common intravenous anesthetic used in clinical,has been shown to regulate cells proliferation,inflammation,angiogenesis and metastasis as well as exerting anti-cancer effect in several different types of cancer.However,the functions and mechanisms of propofol in lactate secretion and invasion of cancer cells remain unknown.The primary aim of this study was to investigate the role of propofol in cells migration and invasion of colon cancer(CRC).Scratches assay,Transwell assay were used to detect colon cells migration and invasion.Realtime Quantitative Polymerase Chain Reaction(RT-qPCR),Western blotting were utilized to detect the expression of related molecules.Dual-luciferase assay was performed to identify transcriptional activity of target genes,RNA pulldown assay was used to confirm the interactions of RNAs.Results indicated that propofol inhibited cell migration and invasion through suppressing lactic acid production in CRC.Moreover,propofol reduced lactic acid production by down-regulating the expression of hypoxia inducible factor-1α(HIF-1α)and monocarboxylate transporter 4(MCT4).In addition,propofol inhibited the expression of HIF-1αand cells migration/invasion via suppressing the interaction of HOX transcript antisense RNA(HOTAIR)with miR-93.The present study indicated that propofol inhibited colon cancer cell migration and invasion by reducing lactic acid production and the expression of HIF-1αand MCT4 via HOTAIR/miR-93 axis.These data may provide potential metabolic targets for CRC treatment.展开更多
Based on the published animal studies,we systematically evaluated the outcomes of various materials for rotator cuff repair in animal models and the potentials of their clinical translation.74 animal studies were fina...Based on the published animal studies,we systematically evaluated the outcomes of various materials for rotator cuff repair in animal models and the potentials of their clinical translation.74 animal studies were finally included,of which naturally derived biomaterials were applied the most widely(50.0%),rats were the most commonly used animal model(47.0%),and autologous tissue demonstrated the best outcomes in all animal models.The biomechanical properties of naturally derived biomaterials(maximum failure load:WMD 18.68[95%CI 7.71-29.66];P=0.001,and stiffness:WMD 1.30[95%CI 0.01-2.60];P=0.048)was statistically significant in the rabbit model.The rabbit model showed better outcomes even though the injury was severer compared with the rat model.展开更多
The 4th Joint Workshop organized by the Chinese Society for Biomaterials(CSBM)and the US Society for Biomaterials(SFB),the US/China Workshop:Regulation,Standards,and Innovation IV was successfully held in Minneapolis ...The 4th Joint Workshop organized by the Chinese Society for Biomaterials(CSBM)and the US Society for Biomaterials(SFB),the US/China Workshop:Regulation,Standards,and Innovation IV was successfully held in Minneapolis on April 5th,2017 during the SFB 2017 Annual Meeting.Following the first three annual workshops hosted in both the US(the 1st at Boston in 2013 and the 2nd at Denver in 2014)and China(the 3rd at Haikou in 2015),it is the first biennial workshop after a joint business meeting between CSBM and US SFB during the 10th World Biomaterials Congress in 2016 determined that future joint workshop will be hosted at each country every other year starting in 2017.展开更多
基金the National Natural Science Foundation of China(81772761,81672540).
文摘Propofol,a common intravenous anesthetic used in clinical,has been shown to regulate cells proliferation,inflammation,angiogenesis and metastasis as well as exerting anti-cancer effect in several different types of cancer.However,the functions and mechanisms of propofol in lactate secretion and invasion of cancer cells remain unknown.The primary aim of this study was to investigate the role of propofol in cells migration and invasion of colon cancer(CRC).Scratches assay,Transwell assay were used to detect colon cells migration and invasion.Realtime Quantitative Polymerase Chain Reaction(RT-qPCR),Western blotting were utilized to detect the expression of related molecules.Dual-luciferase assay was performed to identify transcriptional activity of target genes,RNA pulldown assay was used to confirm the interactions of RNAs.Results indicated that propofol inhibited cell migration and invasion through suppressing lactic acid production in CRC.Moreover,propofol reduced lactic acid production by down-regulating the expression of hypoxia inducible factor-1α(HIF-1α)and monocarboxylate transporter 4(MCT4).In addition,propofol inhibited the expression of HIF-1αand cells migration/invasion via suppressing the interaction of HOX transcript antisense RNA(HOTAIR)with miR-93.The present study indicated that propofol inhibited colon cancer cell migration and invasion by reducing lactic acid production and the expression of HIF-1αand MCT4 via HOTAIR/miR-93 axis.These data may provide potential metabolic targets for CRC treatment.
基金supported by the Natural Science Foundation of China(Number:81873184)the National Innovation and Entrepreneurship Training Program for Undergraduate(No.202110730235).
文摘Based on the published animal studies,we systematically evaluated the outcomes of various materials for rotator cuff repair in animal models and the potentials of their clinical translation.74 animal studies were finally included,of which naturally derived biomaterials were applied the most widely(50.0%),rats were the most commonly used animal model(47.0%),and autologous tissue demonstrated the best outcomes in all animal models.The biomechanical properties of naturally derived biomaterials(maximum failure load:WMD 18.68[95%CI 7.71-29.66];P=0.001,and stiffness:WMD 1.30[95%CI 0.01-2.60];P=0.048)was statistically significant in the rabbit model.The rabbit model showed better outcomes even though the injury was severer compared with the rat model.
文摘The 4th Joint Workshop organized by the Chinese Society for Biomaterials(CSBM)and the US Society for Biomaterials(SFB),the US/China Workshop:Regulation,Standards,and Innovation IV was successfully held in Minneapolis on April 5th,2017 during the SFB 2017 Annual Meeting.Following the first three annual workshops hosted in both the US(the 1st at Boston in 2013 and the 2nd at Denver in 2014)and China(the 3rd at Haikou in 2015),it is the first biennial workshop after a joint business meeting between CSBM and US SFB during the 10th World Biomaterials Congress in 2016 determined that future joint workshop will be hosted at each country every other year starting in 2017.
