食管癌生物标志物预测及临床病理特征分析(英文)

Objective:The aim of this study was to explore the expression of manganese superoxide dismutase(MnSOD) in esophageal squamous cell carcinoma and its relationship with clinicopathological characteristics and its biological behavior.Methods:Immunohistochemical method(SP method),reverse transcription-polymerase chain reaction(RT-PCR) and Western blot were combined to detect the MnSOD protein and mRNA expression in 45 cases of esophageal squamous cell carcinoma and the normal tissue that was 5 cm apart from the edge of esophageal cancer lesion and without documented microscopic invasive cancer.Meanwhile,analysis was performed on the relationship between the pathological features of esophageal cancer and its biological behavior.Results:In esophageal squamous cell carcinoma and normal esophageal tissue,MnSOD protein expression was identified in 31.1%(14/45) and 86.7%(31/45)(P = 0.003),respectively,with the relative expression levels of MnSOD mRNA were 0.310 ± 0.036 and 0.482 ± 0.053(P = 0.000).The longer the lesions and the deeper the invasion,the differentiation would become poorer and the expression level of MnSOD would get lower,indicating that the level of MnSOD protein and mRNA expression were closely related to esophageal squamous cell carcinoma in the length of lesion,depth of invasion,and degree of differentiation(P < 0.05).Nevertheless,it showed no association with the presence of the lymph node metastasis,lesion site and the macroscopic classification(P > 0.05).Conclusion:The MnSOD protein and mRNA expression were both decreased in esophageal squamous cell carcinoma tissue.This may be related to the carcinogenesis and development of esophageal cancer.Detection of the expression of MnSOD would be of clinical significance in understanding the prognosis and guiding therapeutic strategy of esophageal cancer.

BSO联合放射线对食管癌细胞株TE-1的增敏效应(英文)

Objective: The aim of the study was to investigate the sensitizing effect of buthionine sulfoximine (BSO) and radiation on esophageal cancer cell line TE-1. Methods: Methyl thiazolyl tetrazolium (MTT) assay was used to observe the inhibition of BSO and radiation on cell proliferation, and to investigate the sensitizing effect of BSO on esophageal cancer cell line TE-1. Flow cytometry (FCM) was used to observe the effect of BSO and radiation on cell apoptosis and cycle. Reverse transcription polymerase chain reaction (RT-PCR) and Western blot were used to observe the effect of BSO on manganese superoxide dismutase (MnSOD) mRNA and protein expression. Results: BSO could inhibit the proliferation of TE-1 esophageal cancer cells, and had significant dose- and time-dependent radiosensitizing effects on TE-1 esophageal cancer cells. After the combined effects of BSO and radiation on TE-1 cells, the rate of apoptosis and G2/M phase proportion increased significantly, and MnSOD mRNA and protein expression decreased. Conclusion: BSO may reduce MnSOD mRNA and protein expression by affecting TE-1 cell cycle, thus inhibiting and inducing the apoptosis of esophageal cancer cells and enhancing the killing effect of the radiation on esophageal cancer cells.