Calreticulin(CRT)is a multifunctional molecule in both intracellular and extracellular environment.We have previ-ously found that a recombinant CRT fragment(rCRT/39-272)could modulate T cell-mediated immunity in mice ...Calreticulin(CRT)is a multifunctional molecule in both intracellular and extracellular environment.We have previ-ously found that a recombinant CRT fragment(rCRT/39-272)could modulate T cell-mediated immunity in mice via activation and expansion of CD1dhiCD5+B cells as well as induction of CRT-specifi c regulatory antibodies.Anti-body secreting cells(ASCs)are terminally differentiated B cells responsible for producing antibodies to participate in positive immune response as well as immune regula-tion.In this study,we demonstrate that rCRT/39-272 dif-ferentiates murine CD1dhiCD5+B cells into ASCs mark-ed by increased expression of plasma cell-associated transcription factors and production of polyreactive antibodies against DNA and CRT in vitro.Intraperitoneal administration of rCRT/39-272 augmented differentiation of CD1dhiCD5+B cells into ASCs in naïve mice or mice with experimental autoimmune encephalomyelitis.Thus,we propose that ASC differentiation and subsequent an-tibody production of CD1dhiCD5+B cells are key steps in CRT-mediated immunoregulation on infl ammatory T cell responses.展开更多
基金This study was supported by grants from PCSIRT(IRT1075)the National Natural Science Foundation of China(Grant Nos.31370908,31070781,and 31100633)+1 种基金the National Basic Research Program(973 Program)(No.2010CB529102)Foundation of Nature Science of Jiangsu Higher Education Institutions of China(11KJB180011).
文摘Calreticulin(CRT)is a multifunctional molecule in both intracellular and extracellular environment.We have previ-ously found that a recombinant CRT fragment(rCRT/39-272)could modulate T cell-mediated immunity in mice via activation and expansion of CD1dhiCD5+B cells as well as induction of CRT-specifi c regulatory antibodies.Anti-body secreting cells(ASCs)are terminally differentiated B cells responsible for producing antibodies to participate in positive immune response as well as immune regula-tion.In this study,we demonstrate that rCRT/39-272 dif-ferentiates murine CD1dhiCD5+B cells into ASCs mark-ed by increased expression of plasma cell-associated transcription factors and production of polyreactive antibodies against DNA and CRT in vitro.Intraperitoneal administration of rCRT/39-272 augmented differentiation of CD1dhiCD5+B cells into ASCs in naïve mice or mice with experimental autoimmune encephalomyelitis.Thus,we propose that ASC differentiation and subsequent an-tibody production of CD1dhiCD5+B cells are key steps in CRT-mediated immunoregulation on infl ammatory T cell responses.
