为筛选适合澳洲坚果(Macadamia ternifolia F. Muell.)幼龄果园间套种的菠萝[Ananas comosus(Linn.)Merr.]品种,利用随机区组设计,对台农16号、台农17号、金菠萝、台农4号4个优良菠萝品种进行试种试验,调查植株农艺性状、果实性状及品质...为筛选适合澳洲坚果(Macadamia ternifolia F. Muell.)幼龄果园间套种的菠萝[Ananas comosus(Linn.)Merr.]品种,利用随机区组设计,对台农16号、台农17号、金菠萝、台农4号4个优良菠萝品种进行试种试验,调查植株农艺性状、果实性状及品质,并进行相关性分析。结果表明,在澳洲坚果幼龄果园下套种,台农16号菠萝植株高度、叶片宽度、平均单果重、可溶性固形物含量、可溶性糖含量均最高,果肉纤维少,质地嫩滑,风味清甜。综合评价,台农16号是作为澳洲坚果间套种的最适菠萝品种。展开更多
In this paper, several factors that affect the efficiency of in vitro adventitious bud regeneration and Agrobacterium tumefaciens-mediated transformation of F. vesca were studied. The results showed that F. vesca see...In this paper, several factors that affect the efficiency of in vitro adventitious bud regeneration and Agrobacterium tumefaciens-mediated transformation of F. vesca were studied. The results showed that F. vesca seeds germination rate was the highest while seeds were cultured in water, and the germination rate was the lowest while seeds were cultured on MS medium supplemented with hormone; the germination rates that seeds cultured on two and three layers filter paper were higher than that seeds cultured on four and five layers filter paper. In vitro adventitious regeneration efficiency was affected by different explants types. The significant difference was existed between petioles and leaves. When using the same type explants, in vitro adventitious buds regeneration rate and the average number of buds per explant between Ruegen (RE) and Yellow Wonder (YW) had no significant difference. RE to Agrobacterium tumefaciens was more sensitive than YW. Using seedling leaves of RE and YW as materials, an efficient Agrobacterium-mediated transformation system was developed. In this system, the concentration of bacteria was OD600=0.5, the explants were immersed in bacteria broth for 9 min, the co-cultured time was 2 days, and had no pre-cultured time. The percentage of explants with resistant buds of RE and YW was compared. The putative transformed plants were confirmed by PCR.展开更多
文摘为筛选适合澳洲坚果(Macadamia ternifolia F. Muell.)幼龄果园间套种的菠萝[Ananas comosus(Linn.)Merr.]品种,利用随机区组设计,对台农16号、台农17号、金菠萝、台农4号4个优良菠萝品种进行试种试验,调查植株农艺性状、果实性状及品质,并进行相关性分析。结果表明,在澳洲坚果幼龄果园下套种,台农16号菠萝植株高度、叶片宽度、平均单果重、可溶性固形物含量、可溶性糖含量均最高,果肉纤维少,质地嫩滑,风味清甜。综合评价,台农16号是作为澳洲坚果间套种的最适菠萝品种。
基金Supported by the Scientific Research fund of the Guangxi Zhuang Autonomous Region(GXNYRKS201601)
文摘In this paper, several factors that affect the efficiency of in vitro adventitious bud regeneration and Agrobacterium tumefaciens-mediated transformation of F. vesca were studied. The results showed that F. vesca seeds germination rate was the highest while seeds were cultured in water, and the germination rate was the lowest while seeds were cultured on MS medium supplemented with hormone; the germination rates that seeds cultured on two and three layers filter paper were higher than that seeds cultured on four and five layers filter paper. In vitro adventitious regeneration efficiency was affected by different explants types. The significant difference was existed between petioles and leaves. When using the same type explants, in vitro adventitious buds regeneration rate and the average number of buds per explant between Ruegen (RE) and Yellow Wonder (YW) had no significant difference. RE to Agrobacterium tumefaciens was more sensitive than YW. Using seedling leaves of RE and YW as materials, an efficient Agrobacterium-mediated transformation system was developed. In this system, the concentration of bacteria was OD600=0.5, the explants were immersed in bacteria broth for 9 min, the co-cultured time was 2 days, and had no pre-cultured time. The percentage of explants with resistant buds of RE and YW was compared. The putative transformed plants were confirmed by PCR.