目的探讨2型糖尿病(T2DM)患者线粒体呼吸链复合酶Ⅰ、Ⅱ、Ⅲ活性与疲劳之间的潜在关系。方法选取2022年1月1日—2022年12月31日于泗洪县第一人民医院就诊的130例T2DM患者作为研究对象,根据疲劳量表评分分为糖尿病无疲乏组和糖尿病疲乏...目的探讨2型糖尿病(T2DM)患者线粒体呼吸链复合酶Ⅰ、Ⅱ、Ⅲ活性与疲劳之间的潜在关系。方法选取2022年1月1日—2022年12月31日于泗洪县第一人民医院就诊的130例T2DM患者作为研究对象,根据疲劳量表评分分为糖尿病无疲乏组和糖尿病疲乏组。采集患者的血样,进行常规的空腹血糖(FBG)、糖化血红蛋白(HbA1c)检测,分别使用辅酶Q10(CoQ10)酶联免疫试剂盒和线粒体呼吸链复合物活性定量检测试剂盒检测CoQ10和呼吸链复合酶Ⅰ、Ⅱ、Ⅲ的活性。采用独立样本t检验、双相关Pearson检验、ROC曲线、二元logistic回归分析评估各项临床指标与糖尿病疲劳风险的相关性。结果2组患者FBG、HbA1c、24 h平均血糖(24 h MBG)及24 h血糖标准差(24 h SDBG)和简单自测抑郁量表(PHQ-9)、广泛性焦虑量表(GAD-7)评分差异无统计学意义(P>0.05)。疲乏组患者CoQ10和线粒体呼吸链复合酶Ⅰ、Ⅱ、Ⅲ水平均低于无疲乏组患者(P<0.05)。T2DM患者CoQ10水平和线粒体呼吸链复合酶Ⅰ、Ⅱ、Ⅲ活性与疲劳量表评分呈负相关(r=-0.451~-0.906,P<0.05)。性别(女性)、年龄(60~69岁/70~79岁)、病程(10~年)均为影响T2DM患者产生疲劳感的独立危险因素。结论线粒体呼吸链复合酶Ⅰ、Ⅱ、Ⅲ活性降低与T2DM患者疲劳感产生密切相关,而且对T2DM患者疲劳感的产生具有预测价值。展开更多
[Objective] The paper was to explore the effect of agro-antibiotic 211, the active substance produced by streptomyces JD211, on rice resistance to sheath blight. [Method] Through pot experiment, the induction effect o...[Objective] The paper was to explore the effect of agro-antibiotic 211, the active substance produced by streptomyces JD211, on rice resistance to sheath blight. [Method] Through pot experiment, the induction effect of agro-antibiotic 211 on rice resistance against sheath blight was analyzed, and the effect on phenolic metabolites and pathogenesis-related proteins were studied.[Result] The total phenol, phenylalanine ammonialyase, lignin, chitinase and β-1, 3-glucanase of rice leaves treated by agro-antibiotic 211 were slightly higher than that in control. At 96 h post treatment, the activity of β-1, 3-glucanase was 36.84% and 10.48% higher than that of blank control and CK+ Rhizoctonia solani, respectively; at120 h post treatment, the activity of phenylalanine ammonia-lyase were 42.13% and 62.28% higher than that of blank control and CK+ R. solani,respectively. The effect of agro-antibiotic 211 + R. solani was equal or slightly higher than that of Jinggangmycin + R. solani. [Conclusion] Agroantibiotic 211 induced rice resistance against sheath blight, and was closely related to the accumulation of phenolic substances and the increase of pathogenesis-related proteins.展开更多
文摘目的探讨2型糖尿病(T2DM)患者线粒体呼吸链复合酶Ⅰ、Ⅱ、Ⅲ活性与疲劳之间的潜在关系。方法选取2022年1月1日—2022年12月31日于泗洪县第一人民医院就诊的130例T2DM患者作为研究对象,根据疲劳量表评分分为糖尿病无疲乏组和糖尿病疲乏组。采集患者的血样,进行常规的空腹血糖(FBG)、糖化血红蛋白(HbA1c)检测,分别使用辅酶Q10(CoQ10)酶联免疫试剂盒和线粒体呼吸链复合物活性定量检测试剂盒检测CoQ10和呼吸链复合酶Ⅰ、Ⅱ、Ⅲ的活性。采用独立样本t检验、双相关Pearson检验、ROC曲线、二元logistic回归分析评估各项临床指标与糖尿病疲劳风险的相关性。结果2组患者FBG、HbA1c、24 h平均血糖(24 h MBG)及24 h血糖标准差(24 h SDBG)和简单自测抑郁量表(PHQ-9)、广泛性焦虑量表(GAD-7)评分差异无统计学意义(P>0.05)。疲乏组患者CoQ10和线粒体呼吸链复合酶Ⅰ、Ⅱ、Ⅲ水平均低于无疲乏组患者(P<0.05)。T2DM患者CoQ10水平和线粒体呼吸链复合酶Ⅰ、Ⅱ、Ⅲ活性与疲劳量表评分呈负相关(r=-0.451~-0.906,P<0.05)。性别(女性)、年龄(60~69岁/70~79岁)、病程(10~年)均为影响T2DM患者产生疲劳感的独立危险因素。结论线粒体呼吸链复合酶Ⅰ、Ⅱ、Ⅲ活性降低与T2DM患者疲劳感产生密切相关,而且对T2DM患者疲劳感的产生具有预测价值。
基金Supported by National Natural Science Foundation of China(31460469)
文摘[Objective] The paper was to explore the effect of agro-antibiotic 211, the active substance produced by streptomyces JD211, on rice resistance to sheath blight. [Method] Through pot experiment, the induction effect of agro-antibiotic 211 on rice resistance against sheath blight was analyzed, and the effect on phenolic metabolites and pathogenesis-related proteins were studied.[Result] The total phenol, phenylalanine ammonialyase, lignin, chitinase and β-1, 3-glucanase of rice leaves treated by agro-antibiotic 211 were slightly higher than that in control. At 96 h post treatment, the activity of β-1, 3-glucanase was 36.84% and 10.48% higher than that of blank control and CK+ Rhizoctonia solani, respectively; at120 h post treatment, the activity of phenylalanine ammonia-lyase were 42.13% and 62.28% higher than that of blank control and CK+ R. solani,respectively. The effect of agro-antibiotic 211 + R. solani was equal or slightly higher than that of Jinggangmycin + R. solani. [Conclusion] Agroantibiotic 211 induced rice resistance against sheath blight, and was closely related to the accumulation of phenolic substances and the increase of pathogenesis-related proteins.
