BACKGROUND Addison’s disease(AD)is a rare but potentially fatal disease in Western countries,which can easily be misdiagnosed at an early stage.Severe adrenal tuberculosis(TB)may lead to depression in patients.CASE S...BACKGROUND Addison’s disease(AD)is a rare but potentially fatal disease in Western countries,which can easily be misdiagnosed at an early stage.Severe adrenal tuberculosis(TB)may lead to depression in patients.CASE SUMMARY We report a case of primary adrenal insufficiency secondary to adrenal TB with TB in the lungs and skin in a 48-year-old woman.The patient was misdiagnosed with depression because of her depressed mood.She had hyperpigmentation of the skin,nails,mouth,and lips.The final diagnosis was adrenal TB that resulted in the insufficient secretion of adrenocortical hormone.Adrenocortical hormone test,skin biopsy,T cell spot test of TB,and adrenal computed tomography scan were used to confirm the diagnosis.The patient’s condition improved after hormone replacement therapy and TB treatment.CONCLUSION Given the current status of TB in high-burden countries,outpatient doctors should be aware of and pay attention to TB and understand the early symptoms of AD.展开更多
Variation in weather conditions during grain filling has substantial effects on maize kernel weight(KW). The objective of this work was to characterize variation in KW with sowing date-associated weather conditions an...Variation in weather conditions during grain filling has substantial effects on maize kernel weight(KW). The objective of this work was to characterize variation in KW with sowing date-associated weather conditions and examine the relationship between KW, grain filling parameters, and weather factors. Maize was sown on eight sowing dates(SD) at 15–20-day intervals from mid-March to mid-July during 2012 and 2013 in the North China Plain. With sowing date delay, KW increased initially and later declined, and the greatest KW was obtained at SD6 in both years. The increased KW at SD6 was attributed mainly to kernel growth rate(Gmean), and effective grain-filling period(P). Variations in temperature and radiation were the primary factors that influenced KW and grain-filling parameters. When the effective cumulative temperature(AT) and radiation(Ra)during grain filling were 950 °C and 1005.4 MJ m-2, respectively, P and KW were greatest. High temperatures(daily maximum temperature [Tmax] > 30.2 °C) during grain filling under early sowing conditions, or low temperatures(daily minimum temperature [Tmin] < 20.7 °C) under late sowing conditions combined with high diurnal temperature range(Tmax-min> 7.1 °C) decreased kernel growth rate and ultimately final KW. When sowing was performed from May 25 through June 27, higher KW and yield of maize were obtained. We conclude that variations in environmental conditions(temperature and radiation) during grain filling markedly affect growth rate and duration of grain filling and eventually affect kernel weight and yield of maize.展开更多
During seed germination,the cotton chaperone protein HSP24.7 regulates the release,from the mitochondrial electron transport chain,of reactive oxygen species(ROS),a stimulative signal regulating germination.The functi...During seed germination,the cotton chaperone protein HSP24.7 regulates the release,from the mitochondrial electron transport chain,of reactive oxygen species(ROS),a stimulative signal regulating germination.The function of HSP24.7 during vegetative stages remains largely unknown.Here we propose that suppression of Gh HSP24.7 in cotton seedlings increases tolerance to heat and drought stress.Elevation of Gh HSP24.7 was found to be positively associated with endogenous levels of ROS.We identified a new client protein of Gh HSP24.7,cotton lysine deacetylase(Gh HDA14),which is involved in mitochondrial protein modification.Elevated levels of Gh HSP24.7 suppressed deacetylase activity in mitochondria,leading to increased acetylation of mitochondrial proteins enriched in the subunit of Ftype ATPase,V-type ATPase,and cytochrome C reductase,ultimately reducing leaf ATP content.Consequently,in combination with altered ROS content,Gh HSP24.7 transgenic lines were unable to coordinate stomatal closure under stress.The regulation circuit composed of Gh HSP24.7 and Gh HDA14 represents a post-translation level mechanism in plant abiotic stress responses that integrates the regulation of ROS and ATP.展开更多
The genus Brassica contains a rich diversity of species and morphological types,including leaf,root,and oil crops,all of which show substantial phenotypic variation.Both Chinese cabbage and cabbage are typical leaf-ty...The genus Brassica contains a rich diversity of species and morphological types,including leaf,root,and oil crops,all of which show substantial phenotypic variation.Both Chinese cabbage and cabbage are typical leaf-type crops with normal roots.We created translocation lines based on interspecific crosses between Chinese cabbage and cabbage and identified qdh225,which exhibited a swollen-root phenotype.The swollen root of qdh225 contained a large number of granular substances,and the formation of its irregular morphological tissue was caused by a thickening of the phloem.Transcriptomic and metabolomic data suggested that differential expression of genes encoding nine types of enzymes involved in starch and sucrose metabolism caused changes in starch synthesis and degradation in the swollen root.These genes jointly regulated sucrose and starch levels,leading to significant enrichment of starch and soluble proteins in the swollen root and a reduction in the content of soluble sugars such as d-glucose and trehalose 6-phosphate.A significant increase in auxin(IAA)and abscisic acid(ABA)contents and a decrease in gibberellin(GA)content in the swollen root likely promoted the differential expression of genes associated with hormone signal transduction,thereby regulating the development of the swollen root.Taken together,our data suggest that accumulation of IAA and ABA and reduction in GA promote swollen root formation by regulating hormone-mediated signaling,leading to a thickening of phloem,root enlargement,and substantial accumulation of starch and soluble proteins.The latter provide materials,energy,and nutrient sources for the development of swollen roots.展开更多
As a unique hydro-geological phenomenon in permafrost regions,the seepage of supra-permafrost groundwater will carry a large amount of heat and cause differential settlement in the embankment.This paper presents the r...As a unique hydro-geological phenomenon in permafrost regions,the seepage of supra-permafrost groundwater will carry a large amount of heat and cause differential settlement in the embankment.This paper presents the results of a field study monitoring the supra-permafrost groundwater levels on both sides of an embankment in permafrost regions.It describes a two-dimensional coupled hydro-thermal model and uses it to analyze the influence of seepage on its temperature field considering climate warming.The results show that seepage exacerbates permafrost thawing and thickens the active layer.The thermal influence on the sunny side of the embankment toe is more significant than that on the shady side,which will cause differential settlement in the embankment.After 50 years of operation,the embankment soil temperature with seepage during freezing is 0.2C warmer than that without seepage,and the thermal influence diminished with the increase in depth.Additionally,seepage influences the thermal regime in vertical and horizontal directions of the embankment.During freezing seasons,the thaw depth increases,and the horizontal thaw range decreases.During thawing seasons,the thaw range grows both vertically and horizontally.展开更多
Chinese cabbage(Brassica rapa ssp. pekinensis) has a long cultivation history and is one of the vegetable crops with the largest cultivation area in China. However, salt stress severely damages photosynthesis and horm...Chinese cabbage(Brassica rapa ssp. pekinensis) has a long cultivation history and is one of the vegetable crops with the largest cultivation area in China. However, salt stress severely damages photosynthesis and hormone metabolism, nutritional balances, and results in ion toxicity in plants. To better understand the mechanisms of salt-induced growth inhibition in Chinese cabbage, RNA-seq and physiological index determination were conducted to explore the impacts of salt stress on carbon cycle metabolism and photosynthesis in Chinese cabbage. Here, we found that the number of thylakoids and grana lamellae and the content of starch granules and chlorophyll in the leaves of Chinese cabbage under salt stress showed a time-dependent response, first increasing and then decreasing. Chinese cabbage increased the transcript levels of genes related to the photosynthetic apparatus and carbon metabolism under salt stress, probably in an attempt to alleviate damage to the photosynthetic system and enhance CO_(2) fixation and energy metabolism. The transcription of genes related to starch and sucrose synthesis and degradation were also enhanced;this might have been an attempt to maintain intracellular osmotic pressure by increasing soluble sugar concentrations. Soluble sugars could also be used as potential reactive oxygen species(ROS) scavengers, in concert with peroxidase(POD)enzymes, to eliminate ROS that accumulate during metabolic processes. Our study characterizes the synergistic response network of carbon metabolism and photosynthesis under salt stress.展开更多
Design and synthesis of superior cost-effective non-fullerene acceptors(NFAs)are still big challenges for facilitating the commercialization of organic solar cells(OSCs),yet to be realized.Herein,two medium bandgap fu...Design and synthesis of superior cost-effective non-fullerene acceptors(NFAs)are still big challenges for facilitating the commercialization of organic solar cells(OSCs),yet to be realized.Herein,two medium bandgap fully non-fused ring electron acceptors(NFREAs,medium bandgap,i,e.,1,3-1,8 eV),namely PTR-2Cl and PTR-4Cl are synthesized with only four steps by using intramolecular noncovalent interaction central core,structured alkyl side chain orientation linking units and flanking with different electron-withdrawing end group.Among them,PTR-4C1 exhibits increased average electrostatic potential(ESP)difference with polymer donor,enhanced crystallinity and compactπ-πstacking compared with the control molecule PTR-2CI.As a result,the PTR-4Cl-based OSC achieved an impressive power conversion efficiency(PCE)of 14.72%,with a much higher open-circuit voltage(V_(OC))of 0.953 V and significantly improved fill factor(FF)of 0.758,demonstrating one of the best acceptor material in the top-performing fully NFREA-based OSCs with both high PCE and V_(OC).Notably,PTR-4Cl-based cells maintain a good T_80lifetime of its initial PCE after over 936 h under a continuous thermal annealing treatment and over1300 h T_(80)lifetime without encapsulation.This work provides a cost-effective design strategy for NFREAs on obtaining high V_(OC),efficient exciton dissociation,and ordered molecular packing and thus high-efficiency and stable OSCs.展开更多
A four-year field experiment was conducted to investigate the effect of subsoiling depth on root morphology, nitrogen(N), phosphorus(P), and potassium(K) uptake, and grain yield of spring maize. The results indicated ...A four-year field experiment was conducted to investigate the effect of subsoiling depth on root morphology, nitrogen(N), phosphorus(P), and potassium(K) uptake, and grain yield of spring maize. The results indicated that subsoil tillage promoted root development,increased nutrient accumulation, and increased yield. Compared with conventional soil management(CK), root length, root surface area, and root dry weight at 0–80 cm soil depth under subsoil tillage to 30 cm(T1) and subsoil tillage to 50 cm(T2) were significantly increased, especially the proportions of roots in deeper soil. Root length, surface area, and dry weight differed significantly among three treatments in the order of T2 > T1 > CK at the12-leaf and early filling stages. The range of variation of root diameter in different soil layers in T2 treatment was the smallest, suggesting that roots were more likely to grow downwards with deeper subsoil tillage in soil. The accumulation of N, P, and K in subsoil tillage treatment was significantly increased, but the proportions of kernel and straw were different. In a comparison of T1 with T2, the grain accumulated more N and P, while K accumulation in kernel and straw varied in different years. Grain yield and biomass were increased by 12.8% and 14.6% on average in subsoil tillage treatments compared to conventional soil treatment. Although no significant differences between different subsoil tillage depths were observed for nutrient accumulation and grain yield, lodging resistance of plants was significantly improved in subsoil tillage to 50 cm, a characteristic that favors a high and stable yield under extreme environments.展开更多
Crop yield potential can be increased through the use of appropriate agronomic practices. Integrated agronomic practice (IAP) is an effective way to increase maize (Zea mays L.) grain yield and nitrogen use efficiency...Crop yield potential can be increased through the use of appropriate agronomic practices. Integrated agronomic practice (IAP) is an effective way to increase maize (Zea mays L.) grain yield and nitrogen use efficiency (NUE);however, the physiological processes associated with gains in yield potential obtained from IAP, particularly the different under various soil fertility conditions, remain poorly understood. An IAP strategy including optimal planting density, split fertilizer application, and subsoiling tillage was evaluated over two growing seasons to determine whether the effects of IAP on maize yield and NUE differ under different levels of soil fertility. Compared to farmers' practices (FP), IAP increased maize grain yield in 2013 and 2014 by 25% and 28%, respectively, in low soil fertility (LSF) fields and by 36% and 37%, respectively, in high soil fertility (HSF) fields. The large yield gap was attributed mainly to greater dry matter (DM) and N accumulation with IAP than with FP owing to increased leaf area index (LAI) and DM accumulation rate, which were promoted by greater soil mineral N content (Nmin) and root length. Post-silking DM and N accumulation were also greater with IAP than with FP under HSF conditions, accounting for 60% and 43%, respectively, of total biomass and N accumulation;however, no significant differences were found for post-silking DM and N accumulation between IAP and FP under LSF conditions. Thus, the increase in grain yield with IAP was greater under HSF than under LSF. Because of greater grain yield and N uptake, IAP significantly increased N partial factor productivity, agronomic N efficiency, N recovery efficiency, and physiological efficiency of applied N compared to FP, particularly in the HSF fields. These results indicate that considerable further increases in yield and NUE can be obtained by increasing effective soil N content and maize root length to promote post-silking N and DM accumulation in maize planted at high plant density, especially in fields with low soil fertility.展开更多
Straw return is an effective way to improve crop grain yield and potassium(K)use efficiency by increasing soil K content.However,the effects of straw return on soil K supplying capacity,replacement of K fertilizer,and...Straw return is an effective way to improve crop grain yield and potassium(K)use efficiency by increasing soil K content.However,the effects of straw return on soil K supplying capacity,replacement of K fertilizer,and K-use efficiency under maize(Zea mays L.)–rice(Oryza sativa L.)cropping systems are little studied.A two-year field experiment was conducted to determine the physiological determinants of K-use efficiency under straw return with four K fertilization rates.Sr33(straw returned plus 33%of K fertilizer applied)and Sr67(straw returned plus 67%of K fertilizer applied)increased annual crop yields by 1.5%and 3.2%and increased agronomic K-use efficiency by respectively 2.9 and 1.3-fold on average in the two years,compared with the conventional practice S0K100(no straw returned plus normal amounts of K fertilizer applied).The Sr33 and Sr67 treatments resulted in significantly greater equilibrium K concentration ratios(CR0 K)and specifically exchangeable K(KX)values according to quantity/intensity(Q/I)relationship analyses,indicating improvement of the potential soil K supply capacity.However,the Sr67 better maintained the soil exchangeable K level and K balance.The results suggested that K released from maize and rice straw can replace about half of chemical K fertilizer,depending on the available K content in maize–rice cropping system production.展开更多
Lithium metal anode is a promising electrode with high theoretical specific capacity and low electrode potential.However,its unstable interface and low Coulombic efficiency,resulting from the dendritic growth of lithi...Lithium metal anode is a promising electrode with high theoretical specific capacity and low electrode potential.However,its unstable interface and low Coulombic efficiency,resulting from the dendritic growth of lithium,limits its commercial application.PIM-1(PIM:polymer of intrinsic microporosity),which is a polymer with abundant micropores,exhibits high rigidity and flexibility with contorted spirocenters in the backbone,and is an ideal candidate for artificial solid electrolyte interphases(SEI).In this work,a PIM-1 membrane was synthesized and fabricated as a protective membrane on the surface of an electrode to facilitate the uniform flux of Li ions and act as a stable interface for the lithium plating/stripping process.Nodule-like lithium with rounded edges was observed under the PIM-1 membrane.The Li@PIM-1 electrode delivered a high average Coulombic efficiency(99.7%),excellent cyclability(80%capacity retention rate after 600 cycles at 1 C),and superior rate capability(125.3 m Ah g-1 at 10 C).Electrochemical impedance spectrum(EIS)showed that the PIM-1 membrane could lower the diffusion rate of Li+significantly and change the rate-determining step from charge transfer to Li+diffusion.Thus,the PIM-1 membrane is proven to act as an artificial SEI to facilitate uniform and stable deposition of lithium,in favor of obtaining a compact and dense Li-plating pattern.This work extends the application of PIMs in the field of lithium batteries and provides ideas for the construction of artificial SEI.展开更多
Matrine is a promising botanical antifungal;however, the mechanism underlying the antifungal activity is yet limited. We studied the antifungal activity of matrine and the underlying mechanism in Botryosphaeria dothid...Matrine is a promising botanical antifungal;however, the mechanism underlying the antifungal activity is yet limited. We studied the antifungal activity of matrine and the underlying mechanism in Botryosphaeria dothidea as a model strain. Matrine strongly inhibited mycelial growth of B. dothidea in a dose-dependent manner. Matrine-treated B. dothidea showed morphological and ultrastructural alterations, including shriveled hyphae, plasmolysis, and leakage of cytoplasm related to cell membrane deterioration. In addition, matrine caused significantly high conductivity and absorbance (260 nm) in extracellular matrices and low lipid contents in B. dothidea, indicating increased membrane permeability. Lipid peroxidation showed that matrine resulted in increased malondialdehyde content while enhancing the generation of reactive oxygen species and the activities of superoxide dismutase, catalase, and peroxidase. These results showed that matrine inhibited the mycelial growth of B. dothidea by enhancing cell membrane permeability via membrane lipid peroxidation.展开更多
Objective: This study aims to investigate the clinicopathologic significance of lymphatic vessel invasion (LVI) labeled by D2-40 monoclonal antibody in esophageal squamous cell carcinoma (ESCC). Methods: Immunohistoch...Objective: This study aims to investigate the clinicopathologic significance of lymphatic vessel invasion (LVI) labeled by D2-40 monoclonal antibody in esophageal squamous cell carcinoma (ESCC). Methods: Immunohistochemical assay was used to detect the expression of D2-40 and LVI in 107 ESCC patients. Then, the correlation between the clinicopathologic feature and the overall survival time of the patients was analyzed. Results: The lymph node metastasis rates were 70% and 21% in the LVI-positive and LVI-negative groups, respectively. The nodal metastasis rate was higher in the LVI-positive group than in the LVI-negative group. Multivariate regression analysis showed that LVI was related to nodal metastasis (P<0.001). The median survival time of the patients was 26 and 43 months in the LVI-positive and LVI-negative groups, respectively. Although univariate regression analysis showed significant difference between the two groups (P=0.014), multivariate regression analysis revealed that LVI was not an independent prognostic factor for overall survival in the ESCC patients (P=0.062). Lymphatic node metastasis (P=0.031), clinical stage (P=0.019), and residual tumor (P=0.026) were the independent prognostic factors. Conclusion: LVI labeled by D2-40 monoclonal antibody is a risk factor predictive of lymph node metastasis in ESCC patients.展开更多
Genes homologous to members of the MRP gene family in Caenorhabditis elegans are important in drug resistance.To further explore the molecular mechanism of drug resistance in pine wood nematode(Bursaphelenchus xylophi...Genes homologous to members of the MRP gene family in Caenorhabditis elegans are important in drug resistance.To further explore the molecular mechanism of drug resistance in pine wood nematode(Bursaphelenchus xylophilus),we used bioinformatics approaches to analyze genomic data for B.xylophilus and identified Bx-MRP genes.We predicted the structure and function of the genes and encoded proteins.Using bioinformatics programs to predict and analyze various properties of the predicted proteins,including hydrophobicity,transmembrane regions,phosphorylation sites,and topologically isomeric structures,of these Bx-MRP genes,we determined that they function in transmembrane transport.From the results of RT-qPCR,the Bx-MRP family members confer significant differential resistance to different drug treatments.After treatment with different concentrations of emamectin benzoate,avermectin and matrine,the expression of each gene increased with increasing drug concentrations,indicating that the family members play a positive role in the regulation of multidrug resistance.展开更多
Background: The p21-activated kinase 1 (PAK1)is essential of microtubule assembly during oocyte meiotic maturation porcine oocytes. for mitosis and plays an important role in the regulatio in mice; however, little ...Background: The p21-activated kinase 1 (PAK1)is essential of microtubule assembly during oocyte meiotic maturation porcine oocytes. for mitosis and plays an important role in the regulatio in mice; however, little is known about its role in Result: Total p21-activated kinase 1 (PAK1) and phosphorylated PAK1 at Thr423 (PAK1^Thr423) were consistently expressed in porcine oocytes from the germinal vesicle (GV) to the second metaphase (MII) stages, but phosphorylation of histone H3 at Serr10 (H3^ser10) was only expressed after the GV stage. Immunofiuorescence analysis revealed that PAK1Thr423 and H3^ser10 colocalized on chromosomes after the GV stage. Blocking of endogenous PAK1^Thr423 by injecting a specific antibody decreased the phosphorylation level of H3^ser10; however, it had no impact on chromatin condensation, meiotic progression, cleavage rate of blastomeres or the rate of blastocyst formation. Conclusion: Phosphorylation of PAK1^Thr423 is a spontaneous activation process and the activated PAK1^Thr423 can promote the phosphorylation of H3^ser10; however, this pathway is not required for meiotic maturation of porcine oocytes or early embryonic development.展开更多
Buried pipelines are widely used for transporting oil in remote cold regions. However, the warm oil can induce considerable thermal influence on the surrounding frozen soils and result in severe maintenance problems. ...Buried pipelines are widely used for transporting oil in remote cold regions. However, the warm oil can induce considerable thermal influence on the surrounding frozen soils and result in severe maintenance problems. This paper presents a case study of the thermal influence of ponding and buried warm-oil pipelines on permafrost along the China-Russia Crude Oil Pipeline(CRCOP) in Northeast China. Since its operation in 2011, the operation of the warm-oil pipelines has led to rapid warming and thawing of the surrounding permafrost and development of sizable ponding along the pipeline route,which, in return, exacerbates the permafrost degradation. A field study was conducted along a 400-km long segment of the CRCOP in permafrost regions of Northeast China to collect the location and size information of ponding. A two-dimensional heat transfer model coupled with phase change was established to analyze the thermal influence of ponding and the operation of warm-oil pipelines on the surrounding permafrost. In-situ measured ground temperatures from a monitoring site were obtained to validate the numerical model. The simulation results show that ponding accelerates the development of the thaw bulb around the pipeline. The maximum thaw depth below the pipeline increases from 4 m for the case without ponding to 9 m for the case with ponding after 50 years of operation, and ponding directly above the pipe induces the maximum thaw depth. Engineering measures should be adopted to control the size or even eliminate surface water-rich ponding for the long-term performance of buried warm-oil pipelines.展开更多
By large-scale dynamic tests carried out on a traditional sand-gravel embankment at the Beilu River section along the Qinghai-Tibet Railroad, we collected the acceleration waveforms close to the railway tracks when tr...By large-scale dynamic tests carried out on a traditional sand-gravel embankment at the Beilu River section along the Qinghai-Tibet Railroad, we collected the acceleration waveforms close to the railway tracks when trains passed. The dynamic train loading was converted into an equivalent creep stress, using an equivalent static force method. Also, the creep equation of frozen soil was introduced according to the results of frozen soil rheological triaxial tests. A coupled creep model based on a time-hardening power function rule and the Druker-Prager yield and failure criterion was estab- lished to analyze the creep effects of a plain fill embankment under repeated train loads. The temperature field of the embankment in the permafrost area was set at the current geothermal conditions. As a result, the permanent deformation of the embankment under train loading was obtained, and the permanent deformation under the train loads to the total embankment deformation was also analyzed.展开更多
Satellite glial cells surround neurons within dorsal root ganglia. Previous studies have focused on single-cell suspensions of cultured neurons derived from rat dorsal root ganglia. At present, the primary culture met...Satellite glial cells surround neurons within dorsal root ganglia. Previous studies have focused on single-cell suspensions of cultured neurons derived from rat dorsal root ganglia. At present, the primary culture method for satellite glial cells derived from rat dorsal root ganglia requires no digestion skill. Hence, the aim of the present study was to establish a novel primary culture method for satellite glial cells derived from dorsal root ganglia. Neonatal rat spine was collected and an incision made to expose the transverse protrusion and remove dorsal root ganglia. Dorsal root ganglia were freed from nerve fibers, connective tissue, and capsule membranes, then rinsed and transferred to 6-well plates, and cultured in a humidified 5% CO_2 incubator at 37°C. After 3 days in culture, some cells had migrated from dorsal root ganglia. After subculture, cells were identified by immunofluorescence labeling for three satellite glial cell-specific markers: glutamine synthetase, glial fibrillary acidic protein, and S100β. Cultured cells expressed glutamine synthetase, glial fibrillary acidic protein, and S100β, suggesting they are satellite glial cells with a purity of > 95%. Thus, we have successfully established a novel primary culture method for obtaining high-purity satellite glial cells from rat dorsal root ganglia without digestion.展开更多
文摘BACKGROUND Addison’s disease(AD)is a rare but potentially fatal disease in Western countries,which can easily be misdiagnosed at an early stage.Severe adrenal tuberculosis(TB)may lead to depression in patients.CASE SUMMARY We report a case of primary adrenal insufficiency secondary to adrenal TB with TB in the lungs and skin in a 48-year-old woman.The patient was misdiagnosed with depression because of her depressed mood.She had hyperpigmentation of the skin,nails,mouth,and lips.The final diagnosis was adrenal TB that resulted in the insufficient secretion of adrenocortical hormone.Adrenocortical hormone test,skin biopsy,T cell spot test of TB,and adrenal computed tomography scan were used to confirm the diagnosis.The patient’s condition improved after hormone replacement therapy and TB treatment.CONCLUSION Given the current status of TB in high-burden countries,outpatient doctors should be aware of and pay attention to TB and understand the early symptoms of AD.
基金supported by the Special Fund for Agro-scientific Research in the Public Interest(No.201203096)the National Key Technology R&D Program of China(Nos.2013BAD07B00 and 2013BAD08B00)the China Agriculture Research System(No.CARS-02)
文摘Variation in weather conditions during grain filling has substantial effects on maize kernel weight(KW). The objective of this work was to characterize variation in KW with sowing date-associated weather conditions and examine the relationship between KW, grain filling parameters, and weather factors. Maize was sown on eight sowing dates(SD) at 15–20-day intervals from mid-March to mid-July during 2012 and 2013 in the North China Plain. With sowing date delay, KW increased initially and later declined, and the greatest KW was obtained at SD6 in both years. The increased KW at SD6 was attributed mainly to kernel growth rate(Gmean), and effective grain-filling period(P). Variations in temperature and radiation were the primary factors that influenced KW and grain-filling parameters. When the effective cumulative temperature(AT) and radiation(Ra)during grain filling were 950 °C and 1005.4 MJ m-2, respectively, P and KW were greatest. High temperatures(daily maximum temperature [Tmax] > 30.2 °C) during grain filling under early sowing conditions, or low temperatures(daily minimum temperature [Tmin] < 20.7 °C) under late sowing conditions combined with high diurnal temperature range(Tmax-min> 7.1 °C) decreased kernel growth rate and ultimately final KW. When sowing was performed from May 25 through June 27, higher KW and yield of maize were obtained. We conclude that variations in environmental conditions(temperature and radiation) during grain filling markedly affect growth rate and duration of grain filling and eventually affect kernel weight and yield of maize.
基金financially supported by the National Natural Science Foundation of China(NSFC 31901430,31971985)Leading Innovative and Entrepreneur Team Introduction Program of Zhejiang(2019R01002)+1 种基金Fundamental Research Funds for the Central UniversitiesJiangsu Collaborative Innovation Center for Modern Crop Production(JCIC-MCP)。
文摘During seed germination,the cotton chaperone protein HSP24.7 regulates the release,from the mitochondrial electron transport chain,of reactive oxygen species(ROS),a stimulative signal regulating germination.The function of HSP24.7 during vegetative stages remains largely unknown.Here we propose that suppression of Gh HSP24.7 in cotton seedlings increases tolerance to heat and drought stress.Elevation of Gh HSP24.7 was found to be positively associated with endogenous levels of ROS.We identified a new client protein of Gh HSP24.7,cotton lysine deacetylase(Gh HDA14),which is involved in mitochondrial protein modification.Elevated levels of Gh HSP24.7 suppressed deacetylase activity in mitochondria,leading to increased acetylation of mitochondrial proteins enriched in the subunit of Ftype ATPase,V-type ATPase,and cytochrome C reductase,ultimately reducing leaf ATP content.Consequently,in combination with altered ROS content,Gh HSP24.7 transgenic lines were unable to coordinate stomatal closure under stress.The regulation circuit composed of Gh HSP24.7 and Gh HDA14 represents a post-translation level mechanism in plant abiotic stress responses that integrates the regulation of ROS and ATP.
基金This work was supported by the National Natural Science Foundation of China(Grant Nos.31930098 and 32172560)the Hebei Provincial Natural Science Fund for Distinguished Young Scholars(Grant No.C2020204063)the Innovative Research Group Project of Hebei Natural Science Foundation(Grant No.C2020204111).
文摘The genus Brassica contains a rich diversity of species and morphological types,including leaf,root,and oil crops,all of which show substantial phenotypic variation.Both Chinese cabbage and cabbage are typical leaf-type crops with normal roots.We created translocation lines based on interspecific crosses between Chinese cabbage and cabbage and identified qdh225,which exhibited a swollen-root phenotype.The swollen root of qdh225 contained a large number of granular substances,and the formation of its irregular morphological tissue was caused by a thickening of the phloem.Transcriptomic and metabolomic data suggested that differential expression of genes encoding nine types of enzymes involved in starch and sucrose metabolism caused changes in starch synthesis and degradation in the swollen root.These genes jointly regulated sucrose and starch levels,leading to significant enrichment of starch and soluble proteins in the swollen root and a reduction in the content of soluble sugars such as d-glucose and trehalose 6-phosphate.A significant increase in auxin(IAA)and abscisic acid(ABA)contents and a decrease in gibberellin(GA)content in the swollen root likely promoted the differential expression of genes associated with hormone signal transduction,thereby regulating the development of the swollen root.Taken together,our data suggest that accumulation of IAA and ABA and reduction in GA promote swollen root formation by regulating hormone-mediated signaling,leading to a thickening of phloem,root enlargement,and substantial accumulation of starch and soluble proteins.The latter provide materials,energy,and nutrient sources for the development of swollen roots.
基金the National Natural Science Foundation of China(Grant No.42001065)Open Project of State Key Laboratory of Frozen Soils Engineering(Grant No.SKLFSE202106)+1 种基金the Natural Science Foundation of Ningxia(2022AAC03052)the University First-Class Discipline Construction Project of Ningxia,China(Grant No.NXYLXK2021A03).
文摘As a unique hydro-geological phenomenon in permafrost regions,the seepage of supra-permafrost groundwater will carry a large amount of heat and cause differential settlement in the embankment.This paper presents the results of a field study monitoring the supra-permafrost groundwater levels on both sides of an embankment in permafrost regions.It describes a two-dimensional coupled hydro-thermal model and uses it to analyze the influence of seepage on its temperature field considering climate warming.The results show that seepage exacerbates permafrost thawing and thickens the active layer.The thermal influence on the sunny side of the embankment toe is more significant than that on the shady side,which will cause differential settlement in the embankment.After 50 years of operation,the embankment soil temperature with seepage during freezing is 0.2C warmer than that without seepage,and the thermal influence diminished with the increase in depth.Additionally,seepage influences the thermal regime in vertical and horizontal directions of the embankment.During freezing seasons,the thaw depth increases,and the horizontal thaw range decreases.During thawing seasons,the thaw range grows both vertically and horizontally.
基金National Natural Science Foundation of China(Nos.82171030,81870678)Natural Science Foundation of Hunan Province,China(No.2021JJ30925)+1 种基金Health Commission of Hunan Province,China(No.B202307026146)Natural Science Foundation of Hainan Province,China(No.821QN1005)。
基金financially supported by the Natural Science Foundation of Hebei Province-Innovation Group Research Project(Grant No.C2020204111)the National Natural Science Foundation of China(Grant No.31930098)+3 种基金the Science Fund for Distinguished Young Scholars of Hebei Province(Grant No.C2021204049)the Hebei Province Outstanding Youth Fund(Grant No.BJ2021024)the Hebei Provincial Key Research Projects(21326344D)Hebei International Joint Research Base of Modern Agricultural Biotechnology.
文摘Chinese cabbage(Brassica rapa ssp. pekinensis) has a long cultivation history and is one of the vegetable crops with the largest cultivation area in China. However, salt stress severely damages photosynthesis and hormone metabolism, nutritional balances, and results in ion toxicity in plants. To better understand the mechanisms of salt-induced growth inhibition in Chinese cabbage, RNA-seq and physiological index determination were conducted to explore the impacts of salt stress on carbon cycle metabolism and photosynthesis in Chinese cabbage. Here, we found that the number of thylakoids and grana lamellae and the content of starch granules and chlorophyll in the leaves of Chinese cabbage under salt stress showed a time-dependent response, first increasing and then decreasing. Chinese cabbage increased the transcript levels of genes related to the photosynthetic apparatus and carbon metabolism under salt stress, probably in an attempt to alleviate damage to the photosynthetic system and enhance CO_(2) fixation and energy metabolism. The transcription of genes related to starch and sucrose synthesis and degradation were also enhanced;this might have been an attempt to maintain intracellular osmotic pressure by increasing soluble sugar concentrations. Soluble sugars could also be used as potential reactive oxygen species(ROS) scavengers, in concert with peroxidase(POD)enzymes, to eliminate ROS that accumulate during metabolic processes. Our study characterizes the synergistic response network of carbon metabolism and photosynthesis under salt stress.
基金the financial support by Hong Kong Scholar program(XJ2021-038)Young Talent Fund of Xi’an Association for Science and Technology(959202313080)+6 种基金the Natural Science Foundation Research Project of Shaanxi Province(2022JM-269)the Postgraduate Innovation and Practical Ability Training Program of Xi’an Shiyou University(YCS21212144)the National Natural Science Foundation of China(52103221,52172048,12175298)the Shandong Provincial Natural Science Foundation(ZR2021QB179,ZR2021QB024,ZR2021ZD06)the Guangdong Natural Science Foundation of China(2023A1515012323,2023A1515010943)the National Key Research and Development Program of China(2022YFB4200400)funded by MOSTthe Fundamental Research Funds of Shandong University。
文摘Design and synthesis of superior cost-effective non-fullerene acceptors(NFAs)are still big challenges for facilitating the commercialization of organic solar cells(OSCs),yet to be realized.Herein,two medium bandgap fully non-fused ring electron acceptors(NFREAs,medium bandgap,i,e.,1,3-1,8 eV),namely PTR-2Cl and PTR-4Cl are synthesized with only four steps by using intramolecular noncovalent interaction central core,structured alkyl side chain orientation linking units and flanking with different electron-withdrawing end group.Among them,PTR-4C1 exhibits increased average electrostatic potential(ESP)difference with polymer donor,enhanced crystallinity and compactπ-πstacking compared with the control molecule PTR-2CI.As a result,the PTR-4Cl-based OSC achieved an impressive power conversion efficiency(PCE)of 14.72%,with a much higher open-circuit voltage(V_(OC))of 0.953 V and significantly improved fill factor(FF)of 0.758,demonstrating one of the best acceptor material in the top-performing fully NFREA-based OSCs with both high PCE and V_(OC).Notably,PTR-4Cl-based cells maintain a good T_80lifetime of its initial PCE after over 936 h under a continuous thermal annealing treatment and over1300 h T_(80)lifetime without encapsulation.This work provides a cost-effective design strategy for NFREAs on obtaining high V_(OC),efficient exciton dissociation,and ordered molecular packing and thus high-efficiency and stable OSCs.
基金supported by the National Key Technology R&D Program of China(2012BAD04B02,2013BAD07B02,and2011BAD16B10)the Special Fund for Agro-Scientific Research in the Public Interest(201103003 and 201303126-4)the Key Technology R&D Program of Jilin province,China(20126026)
文摘A four-year field experiment was conducted to investigate the effect of subsoiling depth on root morphology, nitrogen(N), phosphorus(P), and potassium(K) uptake, and grain yield of spring maize. The results indicated that subsoil tillage promoted root development,increased nutrient accumulation, and increased yield. Compared with conventional soil management(CK), root length, root surface area, and root dry weight at 0–80 cm soil depth under subsoil tillage to 30 cm(T1) and subsoil tillage to 50 cm(T2) were significantly increased, especially the proportions of roots in deeper soil. Root length, surface area, and dry weight differed significantly among three treatments in the order of T2 > T1 > CK at the12-leaf and early filling stages. The range of variation of root diameter in different soil layers in T2 treatment was the smallest, suggesting that roots were more likely to grow downwards with deeper subsoil tillage in soil. The accumulation of N, P, and K in subsoil tillage treatment was significantly increased, but the proportions of kernel and straw were different. In a comparison of T1 with T2, the grain accumulated more N and P, while K accumulation in kernel and straw varied in different years. Grain yield and biomass were increased by 12.8% and 14.6% on average in subsoil tillage treatments compared to conventional soil treatment. Although no significant differences between different subsoil tillage depths were observed for nutrient accumulation and grain yield, lodging resistance of plants was significantly improved in subsoil tillage to 50 cm, a characteristic that favors a high and stable yield under extreme environments.
基金supported by the Key National Research and Development Program of China (2016YFD0300207, 2016YFD0300103)the China Agriculture Research System (CRRS-02)
文摘Crop yield potential can be increased through the use of appropriate agronomic practices. Integrated agronomic practice (IAP) is an effective way to increase maize (Zea mays L.) grain yield and nitrogen use efficiency (NUE);however, the physiological processes associated with gains in yield potential obtained from IAP, particularly the different under various soil fertility conditions, remain poorly understood. An IAP strategy including optimal planting density, split fertilizer application, and subsoiling tillage was evaluated over two growing seasons to determine whether the effects of IAP on maize yield and NUE differ under different levels of soil fertility. Compared to farmers' practices (FP), IAP increased maize grain yield in 2013 and 2014 by 25% and 28%, respectively, in low soil fertility (LSF) fields and by 36% and 37%, respectively, in high soil fertility (HSF) fields. The large yield gap was attributed mainly to greater dry matter (DM) and N accumulation with IAP than with FP owing to increased leaf area index (LAI) and DM accumulation rate, which were promoted by greater soil mineral N content (Nmin) and root length. Post-silking DM and N accumulation were also greater with IAP than with FP under HSF conditions, accounting for 60% and 43%, respectively, of total biomass and N accumulation;however, no significant differences were found for post-silking DM and N accumulation between IAP and FP under LSF conditions. Thus, the increase in grain yield with IAP was greater under HSF than under LSF. Because of greater grain yield and N uptake, IAP significantly increased N partial factor productivity, agronomic N efficiency, N recovery efficiency, and physiological efficiency of applied N compared to FP, particularly in the HSF fields. These results indicate that considerable further increases in yield and NUE can be obtained by increasing effective soil N content and maize root length to promote post-silking N and DM accumulation in maize planted at high plant density, especially in fields with low soil fertility.
基金supported by the Special Fund for Agroscientific Research in the Public Interest of China(201503122)the National Natural Science Foundation of China(31571622)。
文摘Straw return is an effective way to improve crop grain yield and potassium(K)use efficiency by increasing soil K content.However,the effects of straw return on soil K supplying capacity,replacement of K fertilizer,and K-use efficiency under maize(Zea mays L.)–rice(Oryza sativa L.)cropping systems are little studied.A two-year field experiment was conducted to determine the physiological determinants of K-use efficiency under straw return with four K fertilization rates.Sr33(straw returned plus 33%of K fertilizer applied)and Sr67(straw returned plus 67%of K fertilizer applied)increased annual crop yields by 1.5%and 3.2%and increased agronomic K-use efficiency by respectively 2.9 and 1.3-fold on average in the two years,compared with the conventional practice S0K100(no straw returned plus normal amounts of K fertilizer applied).The Sr33 and Sr67 treatments resulted in significantly greater equilibrium K concentration ratios(CR0 K)and specifically exchangeable K(KX)values according to quantity/intensity(Q/I)relationship analyses,indicating improvement of the potential soil K supply capacity.However,the Sr67 better maintained the soil exchangeable K level and K balance.The results suggested that K released from maize and rice straw can replace about half of chemical K fertilizer,depending on the available K content in maize–rice cropping system production.
基金supported by the Opening Project(SKLACPS-C-21)of the State Key Laboratory of Advanced Chemical Power Source,Guizhou Meiling Power Sources Co.,Ltd.the Program for Innovative and Entrepreneurial team in Zhuhai(ZH01110405160007PWC).
文摘Lithium metal anode is a promising electrode with high theoretical specific capacity and low electrode potential.However,its unstable interface and low Coulombic efficiency,resulting from the dendritic growth of lithium,limits its commercial application.PIM-1(PIM:polymer of intrinsic microporosity),which is a polymer with abundant micropores,exhibits high rigidity and flexibility with contorted spirocenters in the backbone,and is an ideal candidate for artificial solid electrolyte interphases(SEI).In this work,a PIM-1 membrane was synthesized and fabricated as a protective membrane on the surface of an electrode to facilitate the uniform flux of Li ions and act as a stable interface for the lithium plating/stripping process.Nodule-like lithium with rounded edges was observed under the PIM-1 membrane.The Li@PIM-1 electrode delivered a high average Coulombic efficiency(99.7%),excellent cyclability(80%capacity retention rate after 600 cycles at 1 C),and superior rate capability(125.3 m Ah g-1 at 10 C).Electrochemical impedance spectrum(EIS)showed that the PIM-1 membrane could lower the diffusion rate of Li+significantly and change the rate-determining step from charge transfer to Li+diffusion.Thus,the PIM-1 membrane is proven to act as an artificial SEI to facilitate uniform and stable deposition of lithium,in favor of obtaining a compact and dense Li-plating pattern.This work extends the application of PIMs in the field of lithium batteries and provides ideas for the construction of artificial SEI.
基金funded by the Special Fund for Forest-Scientific Research in the Public Interest(No.201304403-4)the Fundamental Research Funds for the Central Universities(2572015AA25)
文摘Matrine is a promising botanical antifungal;however, the mechanism underlying the antifungal activity is yet limited. We studied the antifungal activity of matrine and the underlying mechanism in Botryosphaeria dothidea as a model strain. Matrine strongly inhibited mycelial growth of B. dothidea in a dose-dependent manner. Matrine-treated B. dothidea showed morphological and ultrastructural alterations, including shriveled hyphae, plasmolysis, and leakage of cytoplasm related to cell membrane deterioration. In addition, matrine caused significantly high conductivity and absorbance (260 nm) in extracellular matrices and low lipid contents in B. dothidea, indicating increased membrane permeability. Lipid peroxidation showed that matrine resulted in increased malondialdehyde content while enhancing the generation of reactive oxygen species and the activities of superoxide dismutase, catalase, and peroxidase. These results showed that matrine inhibited the mycelial growth of B. dothidea by enhancing cell membrane permeability via membrane lipid peroxidation.
基金supported by the Science and Technology Development Planning of Shandong Provincethe China Postdoctoral Science Fund (Grant No.2012GGE27088 andNo.2011M500531)
文摘Objective: This study aims to investigate the clinicopathologic significance of lymphatic vessel invasion (LVI) labeled by D2-40 monoclonal antibody in esophageal squamous cell carcinoma (ESCC). Methods: Immunohistochemical assay was used to detect the expression of D2-40 and LVI in 107 ESCC patients. Then, the correlation between the clinicopathologic feature and the overall survival time of the patients was analyzed. Results: The lymph node metastasis rates were 70% and 21% in the LVI-positive and LVI-negative groups, respectively. The nodal metastasis rate was higher in the LVI-positive group than in the LVI-negative group. Multivariate regression analysis showed that LVI was related to nodal metastasis (P<0.001). The median survival time of the patients was 26 and 43 months in the LVI-positive and LVI-negative groups, respectively. Although univariate regression analysis showed significant difference between the two groups (P=0.014), multivariate regression analysis revealed that LVI was not an independent prognostic factor for overall survival in the ESCC patients (P=0.062). Lymphatic node metastasis (P=0.031), clinical stage (P=0.019), and residual tumor (P=0.026) were the independent prognostic factors. Conclusion: LVI labeled by D2-40 monoclonal antibody is a risk factor predictive of lymph node metastasis in ESCC patients.
基金supported by the National Key Research and Development Program of China(2017YFD0600101).
文摘Genes homologous to members of the MRP gene family in Caenorhabditis elegans are important in drug resistance.To further explore the molecular mechanism of drug resistance in pine wood nematode(Bursaphelenchus xylophilus),we used bioinformatics approaches to analyze genomic data for B.xylophilus and identified Bx-MRP genes.We predicted the structure and function of the genes and encoded proteins.Using bioinformatics programs to predict and analyze various properties of the predicted proteins,including hydrophobicity,transmembrane regions,phosphorylation sites,and topologically isomeric structures,of these Bx-MRP genes,we determined that they function in transmembrane transport.From the results of RT-qPCR,the Bx-MRP family members confer significant differential resistance to different drug treatments.After treatment with different concentrations of emamectin benzoate,avermectin and matrine,the expression of each gene increased with increasing drug concentrations,indicating that the family members play a positive role in the regulation of multidrug resistance.
基金supported by grants from the National High-Tech R&D Program (No. 2011AA100303)the National Key Technology R&D Program (No. 2011BAD19B01)the National Natural Science Foundation of China(No. 31271253)
文摘Background: The p21-activated kinase 1 (PAK1)is essential of microtubule assembly during oocyte meiotic maturation porcine oocytes. for mitosis and plays an important role in the regulatio in mice; however, little is known about its role in Result: Total p21-activated kinase 1 (PAK1) and phosphorylated PAK1 at Thr423 (PAK1^Thr423) were consistently expressed in porcine oocytes from the germinal vesicle (GV) to the second metaphase (MII) stages, but phosphorylation of histone H3 at Serr10 (H3^ser10) was only expressed after the GV stage. Immunofiuorescence analysis revealed that PAK1Thr423 and H3^ser10 colocalized on chromosomes after the GV stage. Blocking of endogenous PAK1^Thr423 by injecting a specific antibody decreased the phosphorylation level of H3^ser10; however, it had no impact on chromatin condensation, meiotic progression, cleavage rate of blastomeres or the rate of blastocyst formation. Conclusion: Phosphorylation of PAK1^Thr423 is a spontaneous activation process and the activated PAK1^Thr423 can promote the phosphorylation of H3^ser10; however, this pathway is not required for meiotic maturation of porcine oocytes or early embryonic development.
基金supported by the Strategic Priority Research Program of Chinese Academy of Sciences(Grant No.XDA2003020102)the China Postdoctoral Science Foundation(No.2019M653797)+1 种基金the National Natural Science Foundation of China(No.41630636 and No.41772325)the Major Program of the Bureau of International Cooperation,the Chinese Academy of Sciences(131B62KYSB20170012).
文摘Buried pipelines are widely used for transporting oil in remote cold regions. However, the warm oil can induce considerable thermal influence on the surrounding frozen soils and result in severe maintenance problems. This paper presents a case study of the thermal influence of ponding and buried warm-oil pipelines on permafrost along the China-Russia Crude Oil Pipeline(CRCOP) in Northeast China. Since its operation in 2011, the operation of the warm-oil pipelines has led to rapid warming and thawing of the surrounding permafrost and development of sizable ponding along the pipeline route,which, in return, exacerbates the permafrost degradation. A field study was conducted along a 400-km long segment of the CRCOP in permafrost regions of Northeast China to collect the location and size information of ponding. A two-dimensional heat transfer model coupled with phase change was established to analyze the thermal influence of ponding and the operation of warm-oil pipelines on the surrounding permafrost. In-situ measured ground temperatures from a monitoring site were obtained to validate the numerical model. The simulation results show that ponding accelerates the development of the thaw bulb around the pipeline. The maximum thaw depth below the pipeline increases from 4 m for the case without ponding to 9 m for the case with ponding after 50 years of operation, and ponding directly above the pipe induces the maximum thaw depth. Engineering measures should be adopted to control the size or even eliminate surface water-rich ponding for the long-term performance of buried warm-oil pipelines.
基金supported by the National Key Basic Research Program of China (973 Program) (No. 2012CB026106)the Program for the Innovative Research Group of the National Natural Science Foundation of China (No. 41121061)the Project for Excellence, State Key Laboratory of the National Natural Science Foundation of China (No. 41023003)
文摘By large-scale dynamic tests carried out on a traditional sand-gravel embankment at the Beilu River section along the Qinghai-Tibet Railroad, we collected the acceleration waveforms close to the railway tracks when trains passed. The dynamic train loading was converted into an equivalent creep stress, using an equivalent static force method. Also, the creep equation of frozen soil was introduced according to the results of frozen soil rheological triaxial tests. A coupled creep model based on a time-hardening power function rule and the Druker-Prager yield and failure criterion was estab- lished to analyze the creep effects of a plain fill embankment under repeated train loads. The temperature field of the embankment in the permafrost area was set at the current geothermal conditions. As a result, the permanent deformation of the embankment under train loading was obtained, and the permanent deformation under the train loads to the total embankment deformation was also analyzed.
基金supported by the National Natural Science Foundation of China,No.31560295(to LYL)the Priority Union Foundation of Yunnan Department of Science and Technology and Kunming Medical University of China,No.2015FB098(to JHG)+1 种基金the Project of Major Scientific and Technological Achievements Cultivation of Kunming Medical University of China,No.CGPY201802(to LYL)the Health Science and Technology Plan Projects of Yunnan Province of China,No.2014NS202(to JHG)
文摘Satellite glial cells surround neurons within dorsal root ganglia. Previous studies have focused on single-cell suspensions of cultured neurons derived from rat dorsal root ganglia. At present, the primary culture method for satellite glial cells derived from rat dorsal root ganglia requires no digestion skill. Hence, the aim of the present study was to establish a novel primary culture method for satellite glial cells derived from dorsal root ganglia. Neonatal rat spine was collected and an incision made to expose the transverse protrusion and remove dorsal root ganglia. Dorsal root ganglia were freed from nerve fibers, connective tissue, and capsule membranes, then rinsed and transferred to 6-well plates, and cultured in a humidified 5% CO_2 incubator at 37°C. After 3 days in culture, some cells had migrated from dorsal root ganglia. After subculture, cells were identified by immunofluorescence labeling for three satellite glial cell-specific markers: glutamine synthetase, glial fibrillary acidic protein, and S100β. Cultured cells expressed glutamine synthetase, glial fibrillary acidic protein, and S100β, suggesting they are satellite glial cells with a purity of > 95%. Thus, we have successfully established a novel primary culture method for obtaining high-purity satellite glial cells from rat dorsal root ganglia without digestion.