Embryonic liver fordin is involved in glucose glycolysis of hepatic stellate cell by regulating PI3K/Akt signaling

AIM To investigate the role of embryonic liver fordin(ELF) in liver fibrosis by regulating hepatic stellate cells(HSCs) glucose glycolysis.METHODS The expression of ELF and the glucose glycolysisrelated proteins were evaluated in activated HSCs. si RNA was used to silence ELF expression in activated HSCs in vitro and the subsequent changes in PI3K/Akt signaling and glucose glycolysis-related proteins were observed.RESULTS The expression of ELF increased remarkably in HSCs of the fibrosis mouse model and HSCs that were cultured for 3 wk in vitro. Glucose glycolysis-related proteins showed an obvious increase in the activated HSCs, such as phosphofructokinase, platelet and glucose transporter 1. ELF-si RNA, which perfectly silenced the expression of ELF in activated HSCs, led to the induction of glucose glycolysis-related proteins and extracellular matrix(ECM) components. Moreover, p Akt, which is an important downstream factor in PI3K/Akt signaling, showed a significant change in response to the ELF silencing. The expression of glucose glycolysisrelated proteins and ECM components decreased remarkably when the PI3K/Akt signaling was blocked by Ly294002 in the activated HSCs. CONCLUSION ELF is involved in HSC glucose glycolysis by regulating PI3K/Akt signaling.

AEG1通过激活NF-κB和AP-1诱导肝癌HepG2细胞COX-2表达(英文)

Objective:The aim of this study was to investigate whether astrocyte elevated gene 1 (AEG1) regulates COX-2 expression in human hepatoma HepG2 cells and related pathways involved in this process. Methods:Human hepatoma HepG2 cells were transfected with pcDNA3.1(-)-AEG1 plasmid or psilencer2.0-AEG1-shRNA1 plasmid to up/down-regulate AEG1 expression, pcDNA3.1(-) and psilencer 2.0 empty vector plasmids were transfected respectively as control. Real-time RT-PCR was carried out to measure the expression levels of AEG1 and COX-2 mRNA. The expression levels of AEG1 and COX-2 protein were detected by Western blot. NF-κB signaling was blocked by PDTC, and AP-1 signaling was blocked by curcumin. Results:AEG1 mRNA and protein levels were increased after pcDNA3.1(-)-AEG1 transfection, and decreased after psilencer2.0-AEG1-shRNAs transfection. COX-2 mRNA and protein levels were increased in AEG1-overexpressing cells and decreased in AEG1-knockdown cells. Phosphorylations of p65 and c-jun were up-regulated in AEG1-overexpressing cells. Both PDTC and curcumin reduced COX-2 expression in HepG2 cells with AEG1 overexpression. Conclusion:AEG1-overexpressing and-knockdown HepG2 cells are established successfully. AEG1 could induce COX-2 expression though activating NF-κB and AP-1 in human hepatoma HepG2 cells.

In situ TEM visualization of Ag catalysis in Li-O_(2)nanobatteries

Lithium-oxygen(Li-O_(2))batteries have been considered as an ideal solution to solving the global energy crisis.Silver(Ag)and Agbased catalyst have been extensively studied due to their high catalytic activities in Li-O_(2)batteries.However,it remains a challenge to track the catalytic mechanism during the charge/discharge process.Here,a nanoscale processing method was used to assemble a Li-O_(2)nanobattery in an aberration-corrected environmental transmission electron microscope(ETEM),where a single Ag nanowire(NW)was used as catalyst for O_(2)electrode.A visualization of the lithium ion insertion process during the electrochemical reactions was achieved in this nanobattery.Numerous Ag nanoparticles(NPs)were observed on the surface of the Ag NW,which were covered by the discharge product Li2O_(2).By simultaneously studying the evolution of the interface and the phase transformation,it can be concluded that these Ag NPs wrapped around Ag NW acted as catalyst during the subsequent charge/discharge reaction.Based on these studies,Ag NPs decorated on porous carbon were synthesized,it can simultaneously improve the cycling stability(100 cycles)and the maximum specific capacity(17,371 mAh·g^(−1)at a current density of 100 mA·g^(−1))in a coin cell Li-O_(2)battery.This study suggests that nanoscale Ag may be a promising catalyst for Li-O_(2)battery.

三维建模船体外板加工样箱经验介绍

该文针对船体生产设计,运用3DE平台CATIA系统三维建模,运用CATIA二次开发技术,简化重复性的建模工作和操作流程,采用智能化、参数化、可视化操作方式,快速生成三维模型的外板加工样箱模型及图纸。提高设计人员工作效率,缩短设计周期。为船体设计管理、中间产品加工等关键造船环节提供有价值的依据及参考。

Coaxial Multi-Wavelength Generation in YVO4 Crystal with Stimulated Raman Scattering Excited by a Picosecond-Pulsed 1064 Laser

The multiwavelength characteristics of stimulated Raman scattering(SRS)in YVO4 crystal excited by a picosecond laser at 1064nm are investigated theoretically and experimentally.Laser output with seven wavelengths is achieved coaxially and synchronously at 894,972,1175,1312,1486,1713 and 2022 nm in a YVO4 crystal.The maximum total Raman output energy is as high as 2.77mJ under the pump energy of 7.75mJ.A maximum total Raman conversion efficiency of 47.8%is obtained when the pump energy is 6.54 mj.This is the highest order of Stokes components and the highest output energy generated by YVO4 reported up to date.This work expands the Raman spectrum of YVO4 crystal to the near-IR regime,with seven wavelengths covered at the same time,paving the way for new wavelength generation in the near-IR regime and its multiwavelength application.

Effects of laparoscopic hepatectomy and open hepatectomy on liver function

Objective:To assess the effects of laparoscopic hepatectomy(LH)and open hepatectomy(OH)on liver function of patients.Methods:From July 2016 to December 2017 in our hospital,a total 47 cases of patients match the inclusion and exclusion standards,were divided into laparoscopic hepatectomy(LH group,51)and open hepatectomy(OH group,59)randomly.Data of pre-and post-operative liver function of two groups were compared.Results:On the first,second and third day after surgery,the postoperative medians value of ALT in LH group was significantly reduced than in OH group(113.98U/L vs 133.30U/L,Z=-2.086,P=0.037;97.55U/L vs 189.50U/L,Z=-2.341,P=0.019;62.66U/L vs 109.20U/L,Z=-2.171,P=0.030).Meanwhile,the value of AST in LH group was significantly lower than in OH group(75.70U/L vs 141.00U/L,Z=-2.245,P=0.025;61.25U/L vs 147.20U/L,Z=-3.193,P=0.001;30.85U/L vs 51.20U/L,Z=-2.331,P=0.020).However,the differences of aminotransferase were not statistically significant in two groups on the seventh day after surgery(P>0.05).The degree of elevations of TBIL,DBIL and PT were remarkably lower in LH group than OH group(P<0.05),as well as,all of index recovered in LH group faster than in OH group.Conclusions:Compared with OH,the effects of LH on liver function in patients are lesser,and LH is beneficial for recovery of patients after hepatectomy.

Aryl hydrocarbon receptor:Linking environment to aging process in elderly patients with asthma

Aging is a significant risk factor for various diseases,including asthma,and it often leads to poorer clinical outcomes,particularly in elderly individuals.It is recognized that age-related diseases are due to a time-dependent accumulation of cellular damage,resulting in a progressive decline in cellular and physiological functions and an increased susceptibility to chronic diseases.The effects of aging affect not only the elderly but also those of younger ages,posing significant challenges to global healthcare.Thus,understanding the molecular mechanisms associated with aging in different diseases is essential.One intriguing factor is the aryl hydrocarbon receptor(AhR),which serves as a cytoplasmic receptor and ligand-activated transcription factor and has been linked to the aging process.Here,we review the literature on several major hallmarks of aging,including mitochondrial dysfunction,cellular senescence,autophagy,mitophagy,epigenetic alterations,and microbiome disturbances.Moreover,we provide an overview of the impact of AhR on these hallmarks by mediating responses to environmental exposures,particularly in relation to the immune system.Furthermore,we explore how aging hallmarks affect clinical characteristics,inflammatory features,exacerbations,and the treatment of asthma.It is suggested that AhR signaling may potentially play a role in regulating asthma phenotypes in elderly populations as part of the aging process.

谁是我国的第三类劳动者?基于从属性特征的类型学及其工作条件组间差异分析

平台经济的出现引发了世界各国对平台劳动者法律身份的广泛争议。本研究借鉴了主要国际劳动统计机构对第三类劳动者的统计方法,对7680名外卖骑手的从属性水平进行了实证测量,并从“人格从属性”与“经济依赖性”两个维度对骑手的从属性特征进行了类型化,对不同类型下骑手的收入、工作强度、工作时间、社会保障覆盖率等客观工作条件以及工作压力和工作满意度等主观感受进行了组间比较。研究发现,经济依赖性对骑手工作条件的影响更加显著,而人格从属性则对骑手的主观感受具有显著影响。

Differentiation of Human Bone Marrow Stromal Cells into Neural-Like Cells Induced by Sodium Ferulate in vitro

Human marrow stromal cells （hMSCs） are multipotential stem cells, capable of differentiating into bone, cartilage, fat and muscle. Several recent reports demonstrated that hMSCs have been also differentiated into neural cells. However, only a few reported inducers are applicable for clinical use. This work is to explore the effects of sodium ferulate （SF） on differentiation of hMSCs into neural cells in vitro. We found that hMSCs could be induced to the cells with typical neural morphology when cultured with SF. The cells express neural proteins, such as nestin, neuron-specific enolase （NSE） and glial fibrillary acidic protein （GFAP）. About 30% of the hMSC-derived cells expressed nestin when cultured with SF for 3 h, but no expression was detected after 24 h. The percentages of positive cells for NSE or GFAP were about 67% and 39% separately at 6 h, and reached the plateau phage after treatment with SF for 3 days. The data suggest that SF can induce hMSCs to differentiate into neural-like cells in vitro. Cellular ＆ Molecular Immunology. 2005;2（3）：225-229.

Anti-cyclic citrullinated peptide antibody predicts the development of rheumatoid arthritis in patients with undifferentiated arthritis

Background:Clinical outcomes of undifferentiated arthritis(UA)are diverse,and only 40%of patients with UA develop rheumatoid arthritis(RA)after 3 years.Discovering predictive markers at disease onset for further intervention is critical.Therefore,our objective was to analyze the clinical outcomes of UA and ascertain the predictors for RA development.Methods:We performed a prospective,multi-center study from January 2013 to October 2016 among Chinese patients diagnosed with UA in 22 tertiary-care hospitals.Clinical and serological parameters were obtained at recruitment.Follow-up was undertaken in all patients every 12 weeks for 2 years.Predictive factors of disease progression were identified using multivariate Cox proportional hazards regression.Results:A total of 234 patients were recruited in this study,and 17(7.3%)patients failed to follow up during the study.Among the 217 patients who completed the study,83(38.2%)patients went into remission.UA patients who developed RA had a higher rheumatoid factor(RF)-positivity(42.9%vs.16.8%,χ^2=8.228,P=0.008),anti-cyclic citrullinated peptide(CCP)antibodypositivity(66.7%vs.10.7%,χ^2=43.897,P<0.001),and double-positivity rate of RF and anti-CCP antibody(38.1%vs.4.1%,χ^2=32.131,P<0.001)than those who did not.Anti-CCP antibody but not RF was an independent predictor for RA development(hazard ratio 18.017,95%confidence interval:5.803–55.938;P<0.001).Conclusion:As an independent predictor of RA,anti-CCP antibody should be tested at disease onset in all patients with UA.

Exploring metro vibrancy and its relationship with built environment:a cross-city comparison using multi-source urban data

Recent urban transformations have led to critical reflections on the blighted urban infrastruc-tures and called for re-stimulating vital urban places.Especially,the metro has been recognized as the backbone infrastructure for urban mobility and the associated economy agglomeration.To date,limited research has been devoted to investigating the relationship between metro vitality and built environment in mega-cities empirically.This paper presents a multisource urban data-driven approach to quantify the metro vibrancy and its association with the underlying built environment.Massive smart card data is processed to extract metro ridership,which denotes the vibrancy around the metro station in physical space.Social media check-ins are crawled to measure the vitality of metros in virtual spaces.Both physical and virtual vibrancy are integrated into a holistic metro vibrancy metric using an entropy-based weighting method.Certain built environment characteristics,including land use,transportation and buildings are modeled as independent variables.The significant influences of built environ-mental factors on the metro vibrancy are unraveled using the ordinary least square regression and the spatial lag model.With experiments conducted in Shenzhen,Singapore and London,this study comes up with a conclusion that spatial distributions of metro vibrancy metrics in three cities are spatially autocorrelated.The regression analysis suggests that in all the three cities,more affluent urban areas tend to have higher metro virbrancy,while the road density,land use and buildings tend to impact metro vibrancy in only one or two cities.These results demonstrate the relationship between the metro vibrancy and built environment is affected by complex urban contexts.These findings help us to understand metro vibrancy thus make proper policy to re-stimulate the important metro infrastructure in the future.

DAPR-tree: a distributed spatial data indexing scheme with data access patterns to support Digital Earth initiatives

This paper proposes a novel data indexing scheme,the distributed access pattern R-tree(DAPR-tree),for spatial data retrieval in a distributed computing environment.As compared to traditional distributed indexing schemes,the DAPR-tree introduces the data access patterns during the indexing utilization stage so that a more balanced indexing structure can be provided for spatial applications(e.g.Digital Earth data warehouse).In this new indexing scheme,(a)an indexing penalty matrix is proposed by considering the balance of data number,topology and access load between different indexing nodes;(b)an‘access possibility’element is integrated to a classic‘Master-Client’structure for a distributed indexing environment;and(c)indexing algorithm for the DAPR-tree is provided for index implementations.By using a duplication of official GEOSS Clearinghouse system as a case study,the DAPR-tree was evaluated in a number of scenarios.The results show that our indexing schemes generally outperform(around 9%)traditional distributed indices with the utilization of data access patterns.Finally,we discuss the applicability of the DARP-tree and document DARP-tree shortcomings to encourage researchers pursuing related topics in Big Data indexing for Digital Earth and other geospatial initiatives.