Introduction Hyperbilirubinemia is common in the new born owing to their special metabolism characteristics.Physiologic bilirubin levels are protective because of their antioxidant effects[1],while pathological jaundi...Introduction Hyperbilirubinemia is common in the new born owing to their special metabolism characteristics.Physiologic bilirubin levels are protective because of their antioxidant effects[1],while pathological jaundice is harmful to the health of the neonate.Previously,most pathological studies on the jaundice of neonates have concentrat-展开更多
Objective: To establish an animal model of like-auditory neuropathy in neonatal rat. Methods The ani-mals were injected with phenylhydrazine hydrochloride or saline at 7-day of age. ABR and DPOAE were performed to ass...Objective: To establish an animal model of like-auditory neuropathy in neonatal rat. Methods The ani-mals were injected with phenylhydrazine hydrochloride or saline at 7-day of age. ABR and DPOAE were performed to assess the auditory function. The cochlea basilar membrane stretched preparation and cochlear frozen sections were prepared for immunohistochemical staining to examine the morphological change of hair cells and spiral ganglion cells (SGNs). Results At 7-day age the ABR waveI, III, V, latencies andI-III,I-V IWIs in the experimental group were significantly prolonged compared with those in the control group. The ABR thresholds were also elevated in the experimental group. We found there is no significant differ-ence in DPOAE in phenylhydrazine hydrochloride exposure group compare to control group. The cochlear hair cells showed no signs of loss in both group, but the total number of neurofilaments positive cells in SGNs were significantly reduced in the phenylhydrazine treated animals. Conclusion Our study suggests that phenylhydrazine hydrochloride can change the auditory function and induce peripheral nerve pathology by targeted mainly the SGNs in neonatal rat.展开更多
基金supported by grants from the National Basic Research Program of China(973 Program)(#2012CB9679002011CBA01000)the National Natural Science Foundation of China(NSFC#81271082)
文摘Introduction Hyperbilirubinemia is common in the new born owing to their special metabolism characteristics.Physiologic bilirubin levels are protective because of their antioxidant effects[1],while pathological jaundice is harmful to the health of the neonate.Previously,most pathological studies on the jaundice of neonates have concentrat-
基金supported by grants from the National Basic Research Program of China(973Program)And the National Natural Science Foundation of China(NSFC)(#81271082)
文摘Objective: To establish an animal model of like-auditory neuropathy in neonatal rat. Methods The ani-mals were injected with phenylhydrazine hydrochloride or saline at 7-day of age. ABR and DPOAE were performed to assess the auditory function. The cochlea basilar membrane stretched preparation and cochlear frozen sections were prepared for immunohistochemical staining to examine the morphological change of hair cells and spiral ganglion cells (SGNs). Results At 7-day age the ABR waveI, III, V, latencies andI-III,I-V IWIs in the experimental group were significantly prolonged compared with those in the control group. The ABR thresholds were also elevated in the experimental group. We found there is no significant differ-ence in DPOAE in phenylhydrazine hydrochloride exposure group compare to control group. The cochlear hair cells showed no signs of loss in both group, but the total number of neurofilaments positive cells in SGNs were significantly reduced in the phenylhydrazine treated animals. Conclusion Our study suggests that phenylhydrazine hydrochloride can change the auditory function and induce peripheral nerve pathology by targeted mainly the SGNs in neonatal rat.
