目的评价基于标准化病人的情景模拟教学模式在转型医院实习护生PICC维护培训中的效果。方法将在2020年4月—2022年2月在广西某三级甲等医院乳腺外科实习70名护理实习生分成对照组及试验组。试验组采用基于标准化病人外周静脉置入中心静...目的评价基于标准化病人的情景模拟教学模式在转型医院实习护生PICC维护培训中的效果。方法将在2020年4月—2022年2月在广西某三级甲等医院乳腺外科实习70名护理实习生分成对照组及试验组。试验组采用基于标准化病人外周静脉置入中心静脉导管(peripherally inserted central catheter,PICC)情景模拟教学模式,对照组采用传统的教学模式。观察两组护生的PICC维护理论、操作考试的成绩及护生对教学模式的满意度。结果试验组实习生的理论知识考试、操作考核成绩均高于对照组,差异有统计学意义(P<0.05)。护生的教学模式满意度高于对照组,两组差异有统计学意义(P<0.05)。结论基于标准化病人的PICC维护情景模拟教学模式可有助于提高护生PICC维护水平及临床思维能力,提升转型医院教师的教学能力。展开更多
Objectives By observing γ radioactive 103Pd stent affecting the proliferation and apoptosis of vascular smooth muscle cells （VSMCs） to explore the mechanism of radioactive stent preventing in-stent restenosis. Meth...Objectives By observing γ radioactive 103Pd stent affecting the proliferation and apoptosis of vascular smooth muscle cells （VSMCs） to explore the mechanism of radioactive stent preventing in-stent restenosis. Methods Fifty male New Zealand rabbits were randomized into stent group and 103Pd stent group. Control group was set up. The materials were harvested on 3, 7, 14, 28, 56 days after operation and the following investigations were carried out, including pathomorphology, immunohistochemistry, apoptosis （TUNEL） and in situs hybridization studies. Results ①The severity of the stenosis in 103Pd stent group was less than that of stent group. It was the most obvious on 56th day （P 〈 0.01）. ②The expression of PCNA of 103Pd stent group was lower than that of stent group on 3 to 28 days. It was the most obvious on 7th day, 16.35%±0.79% vs 24.36%±0.55% （P〈 0.01）. ②TUNEL method showed that the 103Pd stent group had much more apoptosis of VSMCs than that of stent group. The highest rate of apoptosis appeared on day 7, 14.72%±0.53% vs 12.42%±1.13% （P〈0.01）. ④ By calculating the ratio of PCNA/apoptosis （P：A）, a much lower ratio was seen in 103Pd-stent group than that of stent group at 3 to 28 days. There was significant statistic difference between two groups （P〈0.05）. ⑤For bcl-2/bax ratio, the result in 103Pd-stent group was lower than that of stent group at 3 to 28 days. It had significant statistic difference （P〈0.05）. Conclusions γ radioactive stent can inhibit the proliferation and accelerate apoptosis of injured media VSMCs. Also it can decrease the ratio of proliferation to apoptosis and relieve the severity of restenosis.展开更多
文摘目的评价基于标准化病人的情景模拟教学模式在转型医院实习护生PICC维护培训中的效果。方法将在2020年4月—2022年2月在广西某三级甲等医院乳腺外科实习70名护理实习生分成对照组及试验组。试验组采用基于标准化病人外周静脉置入中心静脉导管(peripherally inserted central catheter,PICC)情景模拟教学模式,对照组采用传统的教学模式。观察两组护生的PICC维护理论、操作考试的成绩及护生对教学模式的满意度。结果试验组实习生的理论知识考试、操作考核成绩均高于对照组,差异有统计学意义(P<0.05)。护生的教学模式满意度高于对照组,两组差异有统计学意义(P<0.05)。结论基于标准化病人的PICC维护情景模拟教学模式可有助于提高护生PICC维护水平及临床思维能力,提升转型医院教师的教学能力。
文摘Objectives By observing γ radioactive 103Pd stent affecting the proliferation and apoptosis of vascular smooth muscle cells （VSMCs） to explore the mechanism of radioactive stent preventing in-stent restenosis. Methods Fifty male New Zealand rabbits were randomized into stent group and 103Pd stent group. Control group was set up. The materials were harvested on 3, 7, 14, 28, 56 days after operation and the following investigations were carried out, including pathomorphology, immunohistochemistry, apoptosis （TUNEL） and in situs hybridization studies. Results ①The severity of the stenosis in 103Pd stent group was less than that of stent group. It was the most obvious on 56th day （P 〈 0.01）. ②The expression of PCNA of 103Pd stent group was lower than that of stent group on 3 to 28 days. It was the most obvious on 7th day, 16.35%±0.79% vs 24.36%±0.55% （P〈 0.01）. ②TUNEL method showed that the 103Pd stent group had much more apoptosis of VSMCs than that of stent group. The highest rate of apoptosis appeared on day 7, 14.72%±0.53% vs 12.42%±1.13% （P〈0.01）. ④ By calculating the ratio of PCNA/apoptosis （P：A）, a much lower ratio was seen in 103Pd-stent group than that of stent group at 3 to 28 days. There was significant statistic difference between two groups （P〈0.05）. ⑤For bcl-2/bax ratio, the result in 103Pd-stent group was lower than that of stent group at 3 to 28 days. It had significant statistic difference （P〈0.05）. Conclusions γ radioactive stent can inhibit the proliferation and accelerate apoptosis of injured media VSMCs. Also it can decrease the ratio of proliferation to apoptosis and relieve the severity of restenosis.
基金National Natural Science Foundation of China(51872118,51632003)Shandong Province Key Research and Development Plan(2020JMRH0401,2019RKB01018)Shandong Provincial Natural Science Foundation(ZR2018PEM008,ZR2019MEM055)。
