Automatic collecting of phenotypic information from plants has become a trend in breeding and smart agriculture.Targeting mature soybean plants at the harvesting stage,which are dense and overlapping,we have proposed ...Automatic collecting of phenotypic information from plants has become a trend in breeding and smart agriculture.Targeting mature soybean plants at the harvesting stage,which are dense and overlapping,we have proposed the SPP-extractor(soybean plant phenotype extractor)algorithm to acquire phenotypic traits.First,to address the mutual occultation of pods,we augmented the standard YOLOv5s model for target detection with an additional attention mechanism.The resulting model could accurately identify pods and stems and could count the entire pod set of a plant in a single scan.Second,considering that mature branches are usually bent and covered with pods,we designed a branch recognition and measurement module combining image processing,target detection,semantic segmentation,and heuristic search.Experimental results on real plants showed that SPP-extractor achieved respective R^(2) scores of 0.93–0.99 for four phenotypic traits,based on regression on manual measurements.展开更多
Pd-based nanocatalyst is a potential oxygen reduction oxidation(ORR)catalyst because of its high activity in alkaline medium and low cost.In this work,bimetallic Pd Au nanocatalysts are prepared by one-pot hydrotherma...Pd-based nanocatalyst is a potential oxygen reduction oxidation(ORR)catalyst because of its high activity in alkaline medium and low cost.In this work,bimetallic Pd Au nanocatalysts are prepared by one-pot hydrothermal method using triblock pluronic copolymers,poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide)(PEO19-PPO69-PEO19)(P123)as reducer and stabilizer,and heat-treatment method is applied to regulate catalyst structure and improve catalyst activity.The results show that the heat treatment can agglomerate the catalyst to a certain extent,but effectively improve the crystallinity and alloying degree of the catalyst.The ORR performance of the Pd Au nanocatalysts obtained under different heat treatment conditions is systematically investigated.Compared with commercial Pd black and Pd Au catalyst before heat treatment,the ORR performance of Au Pd nanocatalyst obtained after heat treatment for one hour at 500℃ has been enhanced.The Pd Au nanocatalysts after heat treatment also display enhanced anti-methanol toxicity ability in acidic medium.展开更多
Larimichthys crocea is a marine fish species cultured in China.Short-term starvation is often applied to improve the quality of cultured L.crocea,and the expression of ghrelin in tissues of stomach,muscle,brain,intest...Larimichthys crocea is a marine fish species cultured in China.Short-term starvation is often applied to improve the quality of cultured L.crocea,and the expression of ghrelin in tissues of stomach,muscle,brain,intestines,liver,and kidney,involved in starvation response,under starvation conditions were studied to understand the effect of starvation on the expression of ghrelin in L.crocea juveniles.The ghrelin expression was tissue-specific,and expression was significantly higher in the stomach compared to other tissues(P<0.01).Additionally,ghrelin expression in different tissues changed along with prolongation of fasting.In the stomach,ghrelin expression levels increased gradually at the beginning of the fast,and then declined after eight days of fasting.Gene expression in the brain and intestines increased at the beginning of the fast,and then decreased with longer fasting time.Interestingly,ghrelin expression declined at the beginning of the fast,then increased with longer fasting in the kidneys and muscles.These results suggest that ghrelin is involved in starvation response in L.crocea juveniles.This study provids insights into ghrelin function and an important reference for the development of reasonable feeding strategies for L.crocea juveniles.展开更多
In order to further analyze the influence of clearance on the kinematic performance of spatial linkage weft insertion mechanism,it is necessary to study the dynamic characteristics of contact impact force model with t...In order to further analyze the influence of clearance on the kinematic performance of spatial linkage weft insertion mechanism,it is necessary to study the dynamic characteristics of contact impact force model with the variable stiffness and damping coefficient.Firstly,the parameters in the output process of the system are solved by describing of the flexible joint clearance.Then,based on Lankarani-Nikravesh contact force model,the contact impact stiffness and damping coefficient is modified from fixed values to time-varying coefficients.The dynamic model of spatial linkage weft insertion mechanism with modified clearance is established by Lagrange method,and the dynamic characteristics of the system are calculated.The results show that the joint clearance can directly affect the output performance of the mechanism.With the increase of the clearance value,the curve fluctuations of acceleration,driving torque and collision force are obvious,and it will be further intensified with the increase of spindle speed,which greatly affects the stability of mechanism and fabric quality.Finally,the virtual prototype is established by the SolidWorks software and simulated by the ADAMS software.The simulation results are compared with the numerical results,which verifies the accuracy of the modeling method in this paper.展开更多
The dependency of the steady-state yaw rate model on vehicle weight and its distribution is studied in this paper. A speed-dependent adjustment of the yaw rate model is proposed to reduce the yaw rate estimation error...The dependency of the steady-state yaw rate model on vehicle weight and its distribution is studied in this paper. A speed-dependent adjustment of the yaw rate model is proposed to reduce the yaw rate estimation error. This new methodology allows the calibration engineer to minimize the yaw rate estimation error caused by the different weight conditions without going through the calibration process multiple times. It is expected that this modified yaw rate model will improve the performance of Electronic Stability Control (ESC) systems such as response time and robustness.展开更多
Tocopherol is an important lipid-soluble antioxidant beneficial for both human health and plant growth. Here, we fine mapped a major QTLqVE1 affecting γ-tocopherol content in maize kernel, positionally cloned and con...Tocopherol is an important lipid-soluble antioxidant beneficial for both human health and plant growth. Here, we fine mapped a major QTLqVE1 affecting γ-tocopherol content in maize kernel, positionally cloned and confirmed the underlying gene ZmPORB1(por1), as a protochlorophyllide oxidoreductase. A 13.7 kb insertion reduced the tocopherol and chlorophyll content, and the photosynthetic activity by repressing ZmPORB1 expression in embryos of NIL-K22, but did not affect the levels of the tocopherol precursors HGA(homogentisic acid)and PMP(phytyl monophosphate). Furthermore, ZmPORB1 is inducible by low oxygen and light, thereby involved in the hypoxia response in developing embryos. Concurrent with natural hypoxia in embryos, the redox state has been changed with NO increasing and H_(2)O_(2) decreasing, which lowered γ-tocopherol content via scavenging reactive nitrogen species. In conclusion, we proposed that the lower lightharvesting chlorophyll content weakened embryo photosynthesis, leading to fewer oxygen supplies and consequently diverse hypoxic responses including an elevated γ-tocopherol consumption. Our findings shed light on the mechanism for fine-tuning endogenous oxygen concentration in the maize embryo through a novel feedback pathway involving the light and low oxygen regulation of ZmPORB1 expression and chlorophyll content.展开更多
Efficient and accurate health state estimation is crucial for lithium-ion battery(LIB)performance monitoring and economic evaluation.Effectively estimating the health state of LIBs online is the key but is also the mo...Efficient and accurate health state estimation is crucial for lithium-ion battery(LIB)performance monitoring and economic evaluation.Effectively estimating the health state of LIBs online is the key but is also the most difficult task for energy storage systems.With high adaptability and applicability advantages,battery health state estimation based on data-driven techniques has attracted extensive attention from researchers around the world.Artificial neural network(ANN)-based methods are often used for state estimations of LIBs.As one of the ANN methods,the Elman neural network(ENN)model has been improved to estimate the battery state more efficiently and accurately.In this paper,an improved ENN estimation method based on electrochemical impedance spectroscopy(EIS)and cuckoo search(CS)is established as the EIS-CS-ENN model to estimate the health state of LIBs.Also,the paper conducts a critical review of various ANN models against the EIS-CS-ENN model.This demonstrates that the EIS-CS-ENN model outperforms other models.The review also proves that,under the same conditions,selecting appropriate health indicators(HIs)according to the mathematical modeling ability and state requirements are the keys in estimating the health state efficiently.In the calculation process,several evaluation indicators are adopted to analyze and compare the modeling accuracy with other existing methods.Through the analysis of the evaluation results and the selection of HIs,conclusions and suggestions are put forward.Also,the robustness of the EIS-CS-ENN model for the health state estimation of LIBs is verified.展开更多
Adeno-associated virus(AAV)has emerged as a pivotal delivery tool in clinical gene therapy owing to its minimal pathogenicity and ability to establish long-term gene expression in different tissues.Recombinant AAV(rAA...Adeno-associated virus(AAV)has emerged as a pivotal delivery tool in clinical gene therapy owing to its minimal pathogenicity and ability to establish long-term gene expression in different tissues.Recombinant AAV(rAAV)has been engineered for enhanced specificity and developed as a tool for treating various diseases.However,as rAAV is being more widely used as a therapy,the increased demand has created challenges for the existing manufacturing methods.Seven rAAV-based gene therapy products have received regulatory approval,but there continue to be concerns about safely using high-dose viral therapies in humans,including immune responses and adverse effects such as genotoxicity,hepatotoxicity,thrombotic microangiopathy,and neurotoxicity.In this review,we explore AAV biology with an emphasis on current vector engineering strategies and manufacturing technologies.We discuss how rAAVs are being employed in ongoing clinical trials for ocular,neurological,metabolic,hematological,neuromuscular,and cardiovascular diseases as well as cancers.We outline immune responses triggered by rAAV,address associated side effects,and discuss strategies to mitigate these reactions.We hope that discussing recent advancements and current challenges in the field will be a helpful guide for researchers and clinicians navigating the ever-evolving landscape of rAAV-based gene therapy.展开更多
Sika deer are known to prefer oak leaves,which are rich in tannins and toxic to most mammals;however,the genetic mechanisms underlying their unique ability to adapt to living in the jungle are still unclear.In identif...Sika deer are known to prefer oak leaves,which are rich in tannins and toxic to most mammals;however,the genetic mechanisms underlying their unique ability to adapt to living in the jungle are still unclear.In identifying the mechanism responsible for the tolerance of a highly toxic diet,we have made a major advancement by explaining the genome of sika deer.We generated the first high-quality,chromosome-level genome assembly of sika deer and measured the correlation between tannin intake and RNA expression in 15 tissues through 180 experiments.Comparative genome analyses showed that the UGT and CYP gene families are functionally involved in the adaptation of sika deer to high-tannin food,especially the expansion of the UGT family 2 subfamily B of UGT genes.The first chromosome-level assembly and genetic characterization of the tolerance to a highly toxic diet suggest that the sika deer genome may serve as an essential resource for understanding evolutionary events and tannin adaptation.Our study provides a paradigm of comparative expressive genomics that can be applied to the study of unique biological features in non-model animals.展开更多
The field of finance heavily relies on cybersecurity to safeguard its systems and clients from harmful software.The identification of malevolent code within financial software is vital for protecting both the financia...The field of finance heavily relies on cybersecurity to safeguard its systems and clients from harmful software.The identification of malevolent code within financial software is vital for protecting both the financial system and individual clients.Nevertheless,present detection models encounter limitations in their ability to identify malevolent code and its variations,all while encompassing a multitude of parameters.To overcome these obsta-cles,we introduce a lean model for classifying families of malevolent code,formulated on Ghost-DenseNet-SE.This model integrates the Ghost module,DenseNet,and the squeeze-and-excitation(SE)channel domain attention mechanism.It substitutes the standard convolutional layer in DenseNet with the Ghost module,thereby diminishing the model’s size and augmenting recognition speed.Additionally,the channel domain attention mechanism assigns distinctive weights to feature channels,facilitating the extraction of pivotal characteristics of malevolent code and bolstering detection precision.Experimental outcomes on the Malimg dataset indicate that the model attained an accuracy of 99.14%in discerning families of malevolent code,surpassing AlexNet(97.8%)and The visual geometry group network(VGGNet)(96.16%).The proposed model exhibits reduced parameters,leading to decreased model complexity alongside enhanced classification accuracy,rendering it a valuable asset for categorizing malevolent code.展开更多
基金supported by the National Natural Science Foundation of China(62276032,32072016)the Agricultural Science and Technology Innovation Program(ASTIP)of Chinese Academy of Agricultural Sciences。
文摘Automatic collecting of phenotypic information from plants has become a trend in breeding and smart agriculture.Targeting mature soybean plants at the harvesting stage,which are dense and overlapping,we have proposed the SPP-extractor(soybean plant phenotype extractor)algorithm to acquire phenotypic traits.First,to address the mutual occultation of pods,we augmented the standard YOLOv5s model for target detection with an additional attention mechanism.The resulting model could accurately identify pods and stems and could count the entire pod set of a plant in a single scan.Second,considering that mature branches are usually bent and covered with pods,we designed a branch recognition and measurement module combining image processing,target detection,semantic segmentation,and heuristic search.Experimental results on real plants showed that SPP-extractor achieved respective R^(2) scores of 0.93–0.99 for four phenotypic traits,based on regression on manual measurements.
基金Financial supports from the National Natural Science Foundation of China (21503120, 21403126)Hubei Provincial Natural Science Foundation of China (2018CFB659)Innovation Foundation from China Three Gorges University (2019SSPY150)
文摘Pd-based nanocatalyst is a potential oxygen reduction oxidation(ORR)catalyst because of its high activity in alkaline medium and low cost.In this work,bimetallic Pd Au nanocatalysts are prepared by one-pot hydrothermal method using triblock pluronic copolymers,poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide)(PEO19-PPO69-PEO19)(P123)as reducer and stabilizer,and heat-treatment method is applied to regulate catalyst structure and improve catalyst activity.The results show that the heat treatment can agglomerate the catalyst to a certain extent,but effectively improve the crystallinity and alloying degree of the catalyst.The ORR performance of the Pd Au nanocatalysts obtained under different heat treatment conditions is systematically investigated.Compared with commercial Pd black and Pd Au catalyst before heat treatment,the ORR performance of Au Pd nanocatalyst obtained after heat treatment for one hour at 500℃ has been enhanced.The Pd Au nanocatalysts after heat treatment also display enhanced anti-methanol toxicity ability in acidic medium.
基金The Key Research and Development Project of Zhejiang Province under contract Nos 2016C02055-7,2017C02013。
文摘Larimichthys crocea is a marine fish species cultured in China.Short-term starvation is often applied to improve the quality of cultured L.crocea,and the expression of ghrelin in tissues of stomach,muscle,brain,intestines,liver,and kidney,involved in starvation response,under starvation conditions were studied to understand the effect of starvation on the expression of ghrelin in L.crocea juveniles.The ghrelin expression was tissue-specific,and expression was significantly higher in the stomach compared to other tissues(P<0.01).Additionally,ghrelin expression in different tissues changed along with prolongation of fasting.In the stomach,ghrelin expression levels increased gradually at the beginning of the fast,and then declined after eight days of fasting.Gene expression in the brain and intestines increased at the beginning of the fast,and then decreased with longer fasting time.Interestingly,ghrelin expression declined at the beginning of the fast,then increased with longer fasting in the kidneys and muscles.These results suggest that ghrelin is involved in starvation response in L.crocea juveniles.This study provids insights into ghrelin function and an important reference for the development of reasonable feeding strategies for L.crocea juveniles.
基金National Natural Science Foundation of China(No.11402186)Innovative Research Team in University of Tianjin,China(No.TD13-5037)Natural Science Foundation of Tianjin,China(Nos.14JCQNJC05600 and 18JCQNJC05300)。
文摘In order to further analyze the influence of clearance on the kinematic performance of spatial linkage weft insertion mechanism,it is necessary to study the dynamic characteristics of contact impact force model with the variable stiffness and damping coefficient.Firstly,the parameters in the output process of the system are solved by describing of the flexible joint clearance.Then,based on Lankarani-Nikravesh contact force model,the contact impact stiffness and damping coefficient is modified from fixed values to time-varying coefficients.The dynamic model of spatial linkage weft insertion mechanism with modified clearance is established by Lagrange method,and the dynamic characteristics of the system are calculated.The results show that the joint clearance can directly affect the output performance of the mechanism.With the increase of the clearance value,the curve fluctuations of acceleration,driving torque and collision force are obvious,and it will be further intensified with the increase of spindle speed,which greatly affects the stability of mechanism and fabric quality.Finally,the virtual prototype is established by the SolidWorks software and simulated by the ADAMS software.The simulation results are compared with the numerical results,which verifies the accuracy of the modeling method in this paper.
文摘The dependency of the steady-state yaw rate model on vehicle weight and its distribution is studied in this paper. A speed-dependent adjustment of the yaw rate model is proposed to reduce the yaw rate estimation error. This new methodology allows the calibration engineer to minimize the yaw rate estimation error caused by the different weight conditions without going through the calibration process multiple times. It is expected that this modified yaw rate model will improve the performance of Electronic Stability Control (ESC) systems such as response time and robustness.
基金supported by the National Natural Science Foundation of China(32200221,U1901201)the National Key Research and Development Program of China(2022YFD1201502)+1 种基金the Key Area Research and Development Program of Guangdong Province,China(2022B0202060003)Huazhong Agricultural University Scientific&Technological Self-Innovation Foundation。
文摘Tocopherol is an important lipid-soluble antioxidant beneficial for both human health and plant growth. Here, we fine mapped a major QTLqVE1 affecting γ-tocopherol content in maize kernel, positionally cloned and confirmed the underlying gene ZmPORB1(por1), as a protochlorophyllide oxidoreductase. A 13.7 kb insertion reduced the tocopherol and chlorophyll content, and the photosynthetic activity by repressing ZmPORB1 expression in embryos of NIL-K22, but did not affect the levels of the tocopherol precursors HGA(homogentisic acid)and PMP(phytyl monophosphate). Furthermore, ZmPORB1 is inducible by low oxygen and light, thereby involved in the hypoxia response in developing embryos. Concurrent with natural hypoxia in embryos, the redox state has been changed with NO increasing and H_(2)O_(2) decreasing, which lowered γ-tocopherol content via scavenging reactive nitrogen species. In conclusion, we proposed that the lower lightharvesting chlorophyll content weakened embryo photosynthesis, leading to fewer oxygen supplies and consequently diverse hypoxic responses including an elevated γ-tocopherol consumption. Our findings shed light on the mechanism for fine-tuning endogenous oxygen concentration in the maize embryo through a novel feedback pathway involving the light and low oxygen regulation of ZmPORB1 expression and chlorophyll content.
基金supported by the National Natural Science Foundation of China(No.62173281 and No.61801407)the Sichuan Science and Technology Pro-gram(No.2019YFG0427 and No.2023YFG0108)+1 种基金the China Scholarship Council(No.201908515099)the Fund of Robot Technology used for the Special Environment Key Laboratory of Sichuan Province(No.18kftk03).
文摘Efficient and accurate health state estimation is crucial for lithium-ion battery(LIB)performance monitoring and economic evaluation.Effectively estimating the health state of LIBs online is the key but is also the most difficult task for energy storage systems.With high adaptability and applicability advantages,battery health state estimation based on data-driven techniques has attracted extensive attention from researchers around the world.Artificial neural network(ANN)-based methods are often used for state estimations of LIBs.As one of the ANN methods,the Elman neural network(ENN)model has been improved to estimate the battery state more efficiently and accurately.In this paper,an improved ENN estimation method based on electrochemical impedance spectroscopy(EIS)and cuckoo search(CS)is established as the EIS-CS-ENN model to estimate the health state of LIBs.Also,the paper conducts a critical review of various ANN models against the EIS-CS-ENN model.This demonstrates that the EIS-CS-ENN model outperforms other models.The review also proves that,under the same conditions,selecting appropriate health indicators(HIs)according to the mathematical modeling ability and state requirements are the keys in estimating the health state efficiently.In the calculation process,several evaluation indicators are adopted to analyze and compare the modeling accuracy with other existing methods.Through the analysis of the evaluation results and the selection of HIs,conclusions and suggestions are put forward.Also,the robustness of the EIS-CS-ENN model for the health state estimation of LIBs is verified.
基金supported by the Graduate Education Fund of the American Australian Association.G.G.is supported by grants from the University of Massachusetts Chan Medical School(an internal grant)the NIH(R01NS076991-01,P01HL131471-05,R01AI121135,UG3HL147367-01,R01HL097088,R01HL152723-02,U19AI149646-01,and UH3HL147367-04).
文摘Adeno-associated virus(AAV)has emerged as a pivotal delivery tool in clinical gene therapy owing to its minimal pathogenicity and ability to establish long-term gene expression in different tissues.Recombinant AAV(rAAV)has been engineered for enhanced specificity and developed as a tool for treating various diseases.However,as rAAV is being more widely used as a therapy,the increased demand has created challenges for the existing manufacturing methods.Seven rAAV-based gene therapy products have received regulatory approval,but there continue to be concerns about safely using high-dose viral therapies in humans,including immune responses and adverse effects such as genotoxicity,hepatotoxicity,thrombotic microangiopathy,and neurotoxicity.In this review,we explore AAV biology with an emphasis on current vector engineering strategies and manufacturing technologies.We discuss how rAAVs are being employed in ongoing clinical trials for ocular,neurological,metabolic,hematological,neuromuscular,and cardiovascular diseases as well as cancers.We outline immune responses triggered by rAAV,address associated side effects,and discuss strategies to mitigate these reactions.We hope that discussing recent advancements and current challenges in the field will be a helpful guide for researchers and clinicians navigating the ever-evolving landscape of rAAV-based gene therapy.
基金This work was supported by the National Key R&D Program of China(Grant No.2018YFD0502204)the Agricultural Science and Technology Innovation Program of China(Grant No.CAAS-ASTIP-2019-ISAPS)+1 种基金the Special Animal Genetic Resources Platform of National Scientific and Technical Infrastructure Center(Grant No.NSTIC TZDWZYK2019)the Sika deer Genome Project of China(Grant No.20140309016YY).
文摘Sika deer are known to prefer oak leaves,which are rich in tannins and toxic to most mammals;however,the genetic mechanisms underlying their unique ability to adapt to living in the jungle are still unclear.In identifying the mechanism responsible for the tolerance of a highly toxic diet,we have made a major advancement by explaining the genome of sika deer.We generated the first high-quality,chromosome-level genome assembly of sika deer and measured the correlation between tannin intake and RNA expression in 15 tissues through 180 experiments.Comparative genome analyses showed that the UGT and CYP gene families are functionally involved in the adaptation of sika deer to high-tannin food,especially the expansion of the UGT family 2 subfamily B of UGT genes.The first chromosome-level assembly and genetic characterization of the tolerance to a highly toxic diet suggest that the sika deer genome may serve as an essential resource for understanding evolutionary events and tannin adaptation.Our study provides a paradigm of comparative expressive genomics that can be applied to the study of unique biological features in non-model animals.
基金funded by National Natural Science Foundation of China(under Grant No.61905201)。
文摘The field of finance heavily relies on cybersecurity to safeguard its systems and clients from harmful software.The identification of malevolent code within financial software is vital for protecting both the financial system and individual clients.Nevertheless,present detection models encounter limitations in their ability to identify malevolent code and its variations,all while encompassing a multitude of parameters.To overcome these obsta-cles,we introduce a lean model for classifying families of malevolent code,formulated on Ghost-DenseNet-SE.This model integrates the Ghost module,DenseNet,and the squeeze-and-excitation(SE)channel domain attention mechanism.It substitutes the standard convolutional layer in DenseNet with the Ghost module,thereby diminishing the model’s size and augmenting recognition speed.Additionally,the channel domain attention mechanism assigns distinctive weights to feature channels,facilitating the extraction of pivotal characteristics of malevolent code and bolstering detection precision.Experimental outcomes on the Malimg dataset indicate that the model attained an accuracy of 99.14%in discerning families of malevolent code,surpassing AlexNet(97.8%)and The visual geometry group network(VGGNet)(96.16%).The proposed model exhibits reduced parameters,leading to decreased model complexity alongside enhanced classification accuracy,rendering it a valuable asset for categorizing malevolent code.