BACKGROUND Epidemiological surveys on heart failure(HF)in Chinese community are relatively lacking.This study aimed to estimate the prevalence and incidence of HF among community residents in southern China.METHODS Ba...BACKGROUND Epidemiological surveys on heart failure(HF)in Chinese community are relatively lacking.This study aimed to estimate the prevalence and incidence of HF among community residents in southern China.METHODS Baseline data of this prospective study was collected from 2015 to 2017 among 12,013 permanent residents aged≥35years in Guangzhou,China.The same survey process was carried out for individuals aged≥65 years after a three-year follow-up.RESULTS The overall prevalence of HF in community residents aged≥35 years was 1.06%.Male had significantly higher risk of HF prevalence[odds ratio(OR)=1.50,P=0.027].The gender-adjusted risk of HF was 1.48 times higher per 10 years aging.HF prevalence was statistically associated with atrial fibrillation,valvular heart disease,hypertension and chronic obstructive pulmonary disease after adjusting for age and gender(OR=8.30,5.17,1.11,2.28,respectively;all P<0.05).HF incidence in individuals aged≥65 years were 847 per 100,000 person-years.Baseline atrial fibrillation,valvular heart disease,and diabetes mellitus were risk factors for HF incidence for individuals aged≥65 years adjusting for age and gender(OR=5.05,3.99,2.11,respectively;all P<0.05).Besides,residents with new-onset atrial fibrillation and myocardial infarction were at significantly higher risk of progression to HF(OR=14.41,8.54,respectively;all P<0.05).CONCLUSIONS Both pre-existing and new-onset cardiovascular diseases were associated with HF incidence in southern China.Management of related cardiovascular diseases may be helpful to reduce the incidence of HF.展开更多
AIM:To investigate the antiproliferative activity of cinobufacini on human hepatocellular carcinoma HepG2 cells and the possible mechanism of its action.METHODS:HepG2 cells were treated with different concentrations o...AIM:To investigate the antiproliferative activity of cinobufacini on human hepatocellular carcinoma HepG2 cells and the possible mechanism of its action.METHODS:HepG2 cells were treated with different concentrations of cinobufacini.Cell viability was measured by methylthiazolyl tetrazolium(MTT) assay.Cell cycledistribution was analyzed by flow cytometry(FCM).Cytoskeletal and nuclear alterations were observed by fluorescein isothiocyanate-phalloidin and DAPI staining under a laser scanning confocal microscope.Changes in morphology and ultrastructure of cells were detected by atomic force microscopy(AFM) at the nanoscale level.RESULTS:MTT assay indicated that cinobufacini significantly inhibited the viability of HepG2 cells in a dosedependent manner.With the concentration of cinobufacini increasing from 0 to 0.10 mg/m L,the cell viability decreased from 74.9% ± 2.7% to 49.41% ± 2.2% and 39.24% ± 2.1%(P < 0.05).FCM analysis demonstrated cell cycle arrest at S phase induced by cinobufacini.The immunofluorescence studies of cytoskeletal and nuclear morphology showed that after cinobufacini treatment,the regular reorganization of actin filaments in HepG2 cells become chaotic,while the nuclei were not damaged seriously.Additionally,high-resolution AFM imaging revealed that cell morphology and ultrastructure changed a lot after treatment with cinobufacini.It appeared as significant shrinkage and deep pores in the cell membrane,with larger particles and a rougher cell surface.CONCLUSION:Cinobufacini inhibits the viability of HepG2 cells via cytoskeletal destruction and cell membrane toxicity.展开更多
Objective To determine the risk factors for thromboembolism in lower risk patients with non-valvular atrial fibrillation(AF)and low CHA2DS2-VASc scores,which remain undefined.Methods We retrospectively analyzed the ba...Objective To determine the risk factors for thromboembolism in lower risk patients with non-valvular atrial fibrillation(AF)and low CHA2DS2-VASc scores,which remain undefined.Methods We retrospectively analyzed the baseline clinical characteristics,routine laboratory parameters,and echocardiographic measurements of 705 patients(71.1%male;mean age:52.10±9.64 years)with low CHA2DS2-VASc score(0 or 1;1 point for female sex)out of 1346 consecutive patients with non-valvular AF who underwent transesophageal echocardiography(TEE)at Guangdong Cardiovascular Institute between January 2013 and December 2015.Results Patients with left atrial thrombus(LAT)or spontaneous echo contrast(SEC)on TEE(24/705,4%)showed a higher incidence rate of vascular disease(54.2%vs.32.9%,P=0.045)and non-paroxysmal AF(79.2%vs.29.4%,P<0.001),larger left atrial diameter(43.08±4.59 vs.36.02±5.53 mm,P<0.001),and lower left ventricular ejection fraction(58.23±8.82%vs.64.15±7.14%,P<0.001)than those without.Multivariate logistic regression analysis identified left atrial diameter[odds ratio(OR)=1.171,95%confidence interval(CI):1.084–1.265,P<0.001]and non-paroxysmal AF(OR=3.766,95%CI:1.282–11.061,P=0.016)as independent risk factors for LAT/SEC.In ROC curve analysis,a left atrial dimeter cutoff of 37.5 mm yielded 95.0%sensitivity and 62.7%specificity(AUC:0.847,P<0.0001,95%CI:0.793–0.914).Conclusion In patients with non-valvular AF with low CHA2DS2-VASc score,the presence of LAT or SEC was associated with left atrial enlargement,which had moderate predictive value,and non-paroxysmal AF.展开更多
Given a query patch from a novel class,one-shot object detection aims to detect all instances of this class in a target image through the semantic similarity comparison.However,due to the extremely limited guidance in...Given a query patch from a novel class,one-shot object detection aims to detect all instances of this class in a target image through the semantic similarity comparison.However,due to the extremely limited guidance in the novel class as well as the unseen appearance difference between the query and target instances,it is difficult to appropriately exploit their semantic similarity and generalize well.To mitigate this problem,we present a universal Cross-Attention Transformer(CAT)module for accurate and efficient semantic similarity comparison in one-shot object detection.The proposed CAT utilizes the transformer mechanism to comprehensively capture bi-directional correspondence between any paired pixels from the query and the target image,which empowers us to sufficiently exploit their semantic characteristics for accurate similarity comparison.In addition,the proposed CAT enables feature dimensionality compression for inference speedup without performance loss.Extensive experiments on three object detection datasets MS-COCO,PASCAL VOC and FSOD under the one-shot setting demonstrate the effectiveness and efficiency of our model,e.g.,it surpasses CoAE,a major baseline in this task,by 1.0%in average precision(AP)on MS-COCO and runs nearly 2.5 times faster.展开更多
Laboratory experiments are performed to investigate the hydrodynamics around a submerged breakwater due to regular incident waves. With an in-house code, a constrained interpolation profile (CIP)-based model is employ...Laboratory experiments are performed to investigate the hydrodynamics around a submerged breakwater due to regular incident waves. With an in-house code, a constrained interpolation profile (CIP)-based model is employed to simulate this process. The model is built on a Cartesian grid system with the Navier-Stokes equations using a CIP method for the flow solver and an immersed boundary method (IBM) is used for the treatment of the solid body boundary. A more accurate interface capturing scheme, the tangent of hyperbola for interface capturing/slope weighting (THINC/SW) scheme, is used to track the free surface. The numerical results are compared with experimental data. Reasonably good agreement is achieved in terms of the wave profiles at six measuring stations, the flow velocities at three different space locations and the pressures of eight points on the surface of the submerged breakwater. Moreover, the water mass transfer over the breakwater is discussed using a two-phase VOF model and the wave spectrum is also presented for analysis. It is indicated that the present model can accurately predict the hydrodynamic characteristics of the wave over a submerged bar. Furthermore, the experimental data in the present work can provide reliable basic data, including the wave transformations, the velocities and the dynamic pressures, for the validation of other CFD models.展开更多
基金supported by the National Natural Science Foundation of China(No.81870254)the Guangdong Provincial Clinical Research Center for Cardiovascular Disease Foundation(No.2020B1111170011)the Science and Technology Programs of Guangdong Province(No.2019B020230004)。
文摘BACKGROUND Epidemiological surveys on heart failure(HF)in Chinese community are relatively lacking.This study aimed to estimate the prevalence and incidence of HF among community residents in southern China.METHODS Baseline data of this prospective study was collected from 2015 to 2017 among 12,013 permanent residents aged≥35years in Guangzhou,China.The same survey process was carried out for individuals aged≥65 years after a three-year follow-up.RESULTS The overall prevalence of HF in community residents aged≥35 years was 1.06%.Male had significantly higher risk of HF prevalence[odds ratio(OR)=1.50,P=0.027].The gender-adjusted risk of HF was 1.48 times higher per 10 years aging.HF prevalence was statistically associated with atrial fibrillation,valvular heart disease,hypertension and chronic obstructive pulmonary disease after adjusting for age and gender(OR=8.30,5.17,1.11,2.28,respectively;all P<0.05).HF incidence in individuals aged≥65 years were 847 per 100,000 person-years.Baseline atrial fibrillation,valvular heart disease,and diabetes mellitus were risk factors for HF incidence for individuals aged≥65 years adjusting for age and gender(OR=5.05,3.99,2.11,respectively;all P<0.05).Besides,residents with new-onset atrial fibrillation and myocardial infarction were at significantly higher risk of progression to HF(OR=14.41,8.54,respectively;all P<0.05).CONCLUSIONS Both pre-existing and new-onset cardiovascular diseases were associated with HF incidence in southern China.Management of related cardiovascular diseases may be helpful to reduce the incidence of HF.
文摘AIM:To investigate the antiproliferative activity of cinobufacini on human hepatocellular carcinoma HepG2 cells and the possible mechanism of its action.METHODS:HepG2 cells were treated with different concentrations of cinobufacini.Cell viability was measured by methylthiazolyl tetrazolium(MTT) assay.Cell cycledistribution was analyzed by flow cytometry(FCM).Cytoskeletal and nuclear alterations were observed by fluorescein isothiocyanate-phalloidin and DAPI staining under a laser scanning confocal microscope.Changes in morphology and ultrastructure of cells were detected by atomic force microscopy(AFM) at the nanoscale level.RESULTS:MTT assay indicated that cinobufacini significantly inhibited the viability of HepG2 cells in a dosedependent manner.With the concentration of cinobufacini increasing from 0 to 0.10 mg/m L,the cell viability decreased from 74.9% ± 2.7% to 49.41% ± 2.2% and 39.24% ± 2.1%(P < 0.05).FCM analysis demonstrated cell cycle arrest at S phase induced by cinobufacini.The immunofluorescence studies of cytoskeletal and nuclear morphology showed that after cinobufacini treatment,the regular reorganization of actin filaments in HepG2 cells become chaotic,while the nuclei were not damaged seriously.Additionally,high-resolution AFM imaging revealed that cell morphology and ultrastructure changed a lot after treatment with cinobufacini.It appeared as significant shrinkage and deep pores in the cell membrane,with larger particles and a rougher cell surface.CONCLUSION:Cinobufacini inhibits the viability of HepG2 cells via cytoskeletal destruction and cell membrane toxicity.
基金This work was supported by National Key R&D Program of China(No.2018YFC1312501 and No.2018YFC1312502)Key R&D Program of Guangdong Province,China(No.2019B020230004).
文摘Objective To determine the risk factors for thromboembolism in lower risk patients with non-valvular atrial fibrillation(AF)and low CHA2DS2-VASc scores,which remain undefined.Methods We retrospectively analyzed the baseline clinical characteristics,routine laboratory parameters,and echocardiographic measurements of 705 patients(71.1%male;mean age:52.10±9.64 years)with low CHA2DS2-VASc score(0 or 1;1 point for female sex)out of 1346 consecutive patients with non-valvular AF who underwent transesophageal echocardiography(TEE)at Guangdong Cardiovascular Institute between January 2013 and December 2015.Results Patients with left atrial thrombus(LAT)or spontaneous echo contrast(SEC)on TEE(24/705,4%)showed a higher incidence rate of vascular disease(54.2%vs.32.9%,P=0.045)and non-paroxysmal AF(79.2%vs.29.4%,P<0.001),larger left atrial diameter(43.08±4.59 vs.36.02±5.53 mm,P<0.001),and lower left ventricular ejection fraction(58.23±8.82%vs.64.15±7.14%,P<0.001)than those without.Multivariate logistic regression analysis identified left atrial diameter[odds ratio(OR)=1.171,95%confidence interval(CI):1.084–1.265,P<0.001]and non-paroxysmal AF(OR=3.766,95%CI:1.282–11.061,P=0.016)as independent risk factors for LAT/SEC.In ROC curve analysis,a left atrial dimeter cutoff of 37.5 mm yielded 95.0%sensitivity and 62.7%specificity(AUC:0.847,P<0.0001,95%CI:0.793–0.914).Conclusion In patients with non-valvular AF with low CHA2DS2-VASc score,the presence of LAT or SEC was associated with left atrial enlargement,which had moderate predictive value,and non-paroxysmal AF.
基金We appreciated Xuan Jiang for the statistical analysis. This work was supported by National Nature Science Foundation of China （No.81370295）, Science and Technology Program of Guangdong Province, China （No. 2017A02 0215054）, Science and Technology Planning of Guangzhou City, China （No.2014B070705005）. The authors declared no potential conflicts of interest with respect to the research, authorship or publication of this article.
文摘ObjectiveTo 在左 atrial 血栓( LAT )或左 atrial 上评估红房间分发宽度( RDW )的预兆的价值在有纤维性颤动( AF ) .MethodsWe 考察了的非瓣膜的 atrial 的病人的自发的回响对比( LASEC )作为非瓣膜的 AF 被诊断并且经历了 transesophageal echocardiography ( T )在里面的 692 个病人从2014年4月的广东心血管的研究所到2015年12月。基线临床的特征，血常规的实验室测试，心动电流描记器大小是 analyzed.ResultsEighty -- 四个病人在 T 下面与 LAT/LASEC 被检查。吝啬的 RDW 水平与 non-LAT/LASEC 病人相比在 LAT/LASEC 病人是显著地更高的(13.59%
基金This work was supported by National Nature Science Foundation of China （No. 81370295）, Science and Tech- nology Program of Guangdong Province, China （No. 2017A020215054）, Science and Technology Planning of Guangzhou City, China （No. 2014B070705005）.
基金supported by the National Science and Technology Major Project under Grant No.2020AAA0106900the National Natural Science Foundation of China under Grant Nos.U19B2307 and 61876152+1 种基金the Shaanxi Provincial Key Research and Development Program of China under Grant No.2021KWZ-03the Natural Science Basic Research Program of Shaanxi Province of China under Grant No.2021JCW-03.
文摘Given a query patch from a novel class,one-shot object detection aims to detect all instances of this class in a target image through the semantic similarity comparison.However,due to the extremely limited guidance in the novel class as well as the unseen appearance difference between the query and target instances,it is difficult to appropriately exploit their semantic similarity and generalize well.To mitigate this problem,we present a universal Cross-Attention Transformer(CAT)module for accurate and efficient semantic similarity comparison in one-shot object detection.The proposed CAT utilizes the transformer mechanism to comprehensively capture bi-directional correspondence between any paired pixels from the query and the target image,which empowers us to sufficiently exploit their semantic characteristics for accurate similarity comparison.In addition,the proposed CAT enables feature dimensionality compression for inference speedup without performance loss.Extensive experiments on three object detection datasets MS-COCO,PASCAL VOC and FSOD under the one-shot setting demonstrate the effectiveness and efficiency of our model,e.g.,it surpasses CoAE,a major baseline in this task,by 1.0%in average precision(AP)on MS-COCO and runs nearly 2.5 times faster.
基金Project supported by the National Natural Science Foundation of China (Grant No. 51679212)the Natural Science Foundation of Zhejiang Province (Grant No. LR16E090002)the Fundamental Research Funds for the Central Universities (Grant No. 2018QNA4041).
文摘Laboratory experiments are performed to investigate the hydrodynamics around a submerged breakwater due to regular incident waves. With an in-house code, a constrained interpolation profile (CIP)-based model is employed to simulate this process. The model is built on a Cartesian grid system with the Navier-Stokes equations using a CIP method for the flow solver and an immersed boundary method (IBM) is used for the treatment of the solid body boundary. A more accurate interface capturing scheme, the tangent of hyperbola for interface capturing/slope weighting (THINC/SW) scheme, is used to track the free surface. The numerical results are compared with experimental data. Reasonably good agreement is achieved in terms of the wave profiles at six measuring stations, the flow velocities at three different space locations and the pressures of eight points on the surface of the submerged breakwater. Moreover, the water mass transfer over the breakwater is discussed using a two-phase VOF model and the wave spectrum is also presented for analysis. It is indicated that the present model can accurately predict the hydrodynamic characteristics of the wave over a submerged bar. Furthermore, the experimental data in the present work can provide reliable basic data, including the wave transformations, the velocities and the dynamic pressures, for the validation of other CFD models.
