Lightweight,ultra-flexible,and robust crosslinked transition metal carbide(Ti3C2 MXene)coated polyimide(PI)(C-MXene@PI)porous composites are manufactured via a scalable dip-coating followed by chemical crosslinking ap...Lightweight,ultra-flexible,and robust crosslinked transition metal carbide(Ti3C2 MXene)coated polyimide(PI)(C-MXene@PI)porous composites are manufactured via a scalable dip-coating followed by chemical crosslinking approach.In addition to the hydrophobicity,anti-oxidation and extreme-temperature stability,efficient utilization of the intrinsic conductivity of MXene,the interfacial polarization between MXene and PI,and the micrometer-sized pores of the composite foams are achieved.Consequently,the composites show a satisfactory X-band electromagnetic interference(EMI)shielding effectiveness of 22.5 to 62.5 dB at a density of 28.7 to 48.7 mg cm−3,leading to an excellent surface-specific SE of 21,317 dB cm^(2)g^(−1).Moreover,the composite foams exhibit excellent electrothermal performance as flexible heaters in terms of a prominent,rapid reproducible,and stable electrothermal effect at low voltages and superior heat performance and more uniform heat distribution compared with the commercial heaters composed of alloy plates.Furthermore,the composite foams are well attached on a human body to check their electromechanical sensing performance,demonstrating the sensitive and reliable detection of human motions as wearable sensors.The excellent EMI shielding performance and multifunctionalities,along with the facile and easy-to-scalable manufacturing techniques,imply promising perspectives of the porous C-MXene@PI composites in next-generation flexible electronics,aerospace,and smart devices.展开更多
After teeth are replanted,there are two possible healing responses：periodontal ligament healing or ankylosis with subsequent replacement resorption.The purpose of this study was to compare the fatigue resistance of v...After teeth are replanted,there are two possible healing responses：periodontal ligament healing or ankylosis with subsequent replacement resorption.The purpose of this study was to compare the fatigue resistance of vertically fractured teeth after bonding the fragments under conditions simulating both healing modes.Thirty-two human premolars were vertically fractured and the fragments were bonded together with Super-Bond C＆B.They were then randomly distributed into four groups（BP,CP,CA,BA）.The BP and CP groups were used to investigate the periodontal ligament healing mode whilst the BA and CA groups simulated ankylosis.All teeth had root canal treatment performed.Metal crowns were constructed for the CP and CA groups.The BP and BA groups only had composite resin restorations in the access cavities.All specimens were subjected to a 260 N load at 4 Hz until failure of the bond or until 2 x 106 cycles had been reached if no fracture occurred.Cracks were detected by stereomicroscope imaging and also assessed via dye penetration tests.Finally,interfaces of the resin luting agent were examined by scanning electron microscope.The results confirmed that the fatigue resistance was higher in the groups with simulated periodontal ligament healing.Periodontal reattachment showed important biomechanical role in bonded and replanted vertically fractured teeth.展开更多
Objective To explore the potential reporter gene assay for the detection of sodium channel-specific toxins in shellfish as an alternative for screening harmful algal bloom (HAB) toxins, considering the fact that the e...Objective To explore the potential reporter gene assay for the detection of sodium channel-specific toxins in shellfish as an alternative for screening harmful algal bloom (HAB) toxins, considering the fact that the existing methods including HPLC and bioassay are inappropriate for identifying HAB toxins which poses a serious problem on human health and shellfish industry. Methods A reporter plasmid pEGFP-c-fos containing c-fos promoter and EGFP was constructed and transfected into T24 cells using LipofectAMINE 2000. Positive transfcctants were screened by G418 to produce a pEGFP-c-fos-T24 cell line. After addition of increasing neurotoxic shellfish poison (NSP) or GTX2,3, primary components of paralytic shellfish poison (PSP), changes in expression of EGFP in the cell line were observed under a laser scanning confocal microscope and quantified with Image-pro Plus software. Results Dose-dependent changes in the intensity of green fluorescence were observed for NSP in a range from 0 to 10 ng/mL and for GTX2,3 from 0 to 16 ng/mL. Conclusion pEGFP-c-fos-T24 can be applied in detecting HAB toxins, and cell-based assay can be used as an alternative for screening sodium channel-specific HAB toxins.展开更多
基金support of the Qilu Young Scholar Program of Shandong University(No.31370082163127)the authors acknowledge funding from the support from the Chinese Scholarship Council(to NW,project#201709370040).
文摘Lightweight,ultra-flexible,and robust crosslinked transition metal carbide(Ti3C2 MXene)coated polyimide(PI)(C-MXene@PI)porous composites are manufactured via a scalable dip-coating followed by chemical crosslinking approach.In addition to the hydrophobicity,anti-oxidation and extreme-temperature stability,efficient utilization of the intrinsic conductivity of MXene,the interfacial polarization between MXene and PI,and the micrometer-sized pores of the composite foams are achieved.Consequently,the composites show a satisfactory X-band electromagnetic interference(EMI)shielding effectiveness of 22.5 to 62.5 dB at a density of 28.7 to 48.7 mg cm−3,leading to an excellent surface-specific SE of 21,317 dB cm^(2)g^(−1).Moreover,the composite foams exhibit excellent electrothermal performance as flexible heaters in terms of a prominent,rapid reproducible,and stable electrothermal effect at low voltages and superior heat performance and more uniform heat distribution compared with the commercial heaters composed of alloy plates.Furthermore,the composite foams are well attached on a human body to check their electromechanical sensing performance,demonstrating the sensitive and reliable detection of human motions as wearable sensors.The excellent EMI shielding performance and multifunctionalities,along with the facile and easy-to-scalable manufacturing techniques,imply promising perspectives of the porous C-MXene@PI composites in next-generation flexible electronics,aerospace,and smart devices.
基金supported by the Jiangsu Province's Outstanding Medical Academic Leader Program(No.LJ201110)the National Natural Scientific Foundation of China(81070839)Key Project supported by Medical Science and Technology Development Foundation,Nanjing Department of Health(ZKX10030)
文摘After teeth are replanted,there are two possible healing responses：periodontal ligament healing or ankylosis with subsequent replacement resorption.The purpose of this study was to compare the fatigue resistance of vertically fractured teeth after bonding the fragments under conditions simulating both healing modes.Thirty-two human premolars were vertically fractured and the fragments were bonded together with Super-Bond C＆B.They were then randomly distributed into four groups（BP,CP,CA,BA）.The BP and CP groups were used to investigate the periodontal ligament healing mode whilst the BA and CA groups simulated ankylosis.All teeth had root canal treatment performed.Metal crowns were constructed for the CP and CA groups.The BP and BA groups only had composite resin restorations in the access cavities.All specimens were subjected to a 260 N load at 4 Hz until failure of the bond or until 2 x 106 cycles had been reached if no fracture occurred.Cracks were detected by stereomicroscope imaging and also assessed via dye penetration tests.Finally,interfaces of the resin luting agent were examined by scanning electron microscope.The results confirmed that the fatigue resistance was higher in the groups with simulated periodontal ligament healing.Periodontal reattachment showed important biomechanical role in bonded and replanted vertically fractured teeth.
基金supported by the National Natural Science Foundation of China (U0733006, 40976065)973 Plan of China (2010CB428702)
文摘Objective To explore the potential reporter gene assay for the detection of sodium channel-specific toxins in shellfish as an alternative for screening harmful algal bloom (HAB) toxins, considering the fact that the existing methods including HPLC and bioassay are inappropriate for identifying HAB toxins which poses a serious problem on human health and shellfish industry. Methods A reporter plasmid pEGFP-c-fos containing c-fos promoter and EGFP was constructed and transfected into T24 cells using LipofectAMINE 2000. Positive transfcctants were screened by G418 to produce a pEGFP-c-fos-T24 cell line. After addition of increasing neurotoxic shellfish poison (NSP) or GTX2,3, primary components of paralytic shellfish poison (PSP), changes in expression of EGFP in the cell line were observed under a laser scanning confocal microscope and quantified with Image-pro Plus software. Results Dose-dependent changes in the intensity of green fluorescence were observed for NSP in a range from 0 to 10 ng/mL and for GTX2,3 from 0 to 16 ng/mL. Conclusion pEGFP-c-fos-T24 can be applied in detecting HAB toxins, and cell-based assay can be used as an alternative for screening sodium channel-specific HAB toxins.
基金Project supported by the National Natural Science Foundation of China （No. 31100513）, the Key International Cooperative Project of CN-USA from the Ministry of Science and Technology of China （No. 2010DFB 33960）, the Key Project of Ningbo Science and Technology Bureau of Zhejiang Province （No. 2012C10003）, the Key Project of the Ministry of Science and Technology of China （No. 2012BAC17B02）, and the Fundamental Research Funds for the Central Universities, China
