Effect of WNT5A on epithelial-mesenchymal transition and its correlation with tumor invasion and metastasis in nasopharyngeal carcinoma

Objective:To investigate the correlation between nasopharyngeal carcinoma cell WNT5A and epithelial-mesenchytnal transition(emt)/metastasis,and investigate its possible mechanisms.Methods:RT-PCR and gene transfection were used to detect the expression of nasopharyngeal carcinoma cell strains WNT5A and EMT related factor 5-8F.Transient transfcction of NPC cell line 5-8F was determined by liposome of plasmid with WNT5A gene.The differential expressions of WNTSA and EMT-related factors in cells before and after transfection were detected by RTPCR.Cell scratch assay and Transwell assay were used to detect the motility abilities of cells before and after 5-8F transfection.Results:The expressions of WNT5A and EMT related factors matrix metalloproleinasc-2 of the WNTSA transferred group in the nasopharyngeal carcinoma cell line 5-8F were higher than the blank control group and the empty vector transferred group,and the transfer ability of the WNTSA transferred group was higher than that in the blank control group and the empty vector transferred group,while the expressions of EMT related factors E-cadherin were lower than that in the blank control group and the empty vector transferred group,and the transfer ability of the WNT5A transferred group was higher than that in the blank control group and the empty vector transferred group.Conclusions:In nasopharyngeal carcinoma cells,WNT5A can regulate the epithelial-mesenchymal transition and affect the ability of tumor invasion and metastasis of nasopharyngeal carcinoma.

Vascular complications following prophylactic balloon occlusion of the internal iliac arteries resolved by successful interventional thrombolysis in a patient with morbidly adherent placenta

The increasing incidence of morbidly adherent placenta （MAP） is placing women at a higher risk of life-threatening massive hemorrhage. The involvement of interventional radiology to manage this complex condition by performing prophylactic lilac artery balloon occlusion has been reported recently. However, the effectiveness and safety of this technique have not been fully determined. Here we report the case of a 25-year-old woman with placenta increta with preemptive bilateral internal lilac artery balloons who had external lilac artery thrombosis detected by computed tomography angiography （CTA） 72 h post cesarean section. A digital subtraction angiogram （DSA） and intra-arterial thrombolysis were instantly performed followed by supplementary conservative treatments, leading to a desirable resolution, of thrombus without sequela. This is the first report of vascular complications with successful interventional thrombolysis in this setting. Our experience suggests that prophylactic lilac artery balloon occlusion should be used cautiously in cases of MAP and consideration given to minimizing vascular complications given the hypercoagulable state of pregnancy.

Antepartum hemorrhage from previous-cesarean-sectioned uterus as a potential sign of uterine artery pseudoaneurysm

目的:子宫动脉瘤多被认为与产程中创伤性操作相关,但近年陆续有报道显示子宫动脉瘤可发生于自然分娩过程中。本文将为自然分娩过程中子宫动脉瘤的发生和紧急处理等提供参考指导。创新点:产前出血可能是子宫动脉瘤发生的潜在危险信号。方法:病例报道和文献汇纳分析。患者女,37岁,疤痕子宫,临产入院,产前出血,经阴顺产后12 h,突发阴道大量出血,常规对症处理(按摩子宫、阴道填塞、缩宫素静滴和欣母佩宫颈注射等)未能有效缓解,40 min内出血达1800 ml。床边B超未提示动脉瘤特征,数字减影血管造影(DSA)显示左侧子宫动脉瘤,即刻予动脉栓塞成功止血。患者产后4 d无并发症出院,随访无后遗症。结论:临床医师需要加强对子宫动脉瘤发生的警惕认识和急救处理水平。子宫动脉瘤和产程中的有创性操作无必然相关性,疤痕子宫的产前出血可能是子宫动脉瘤发生的潜在危险信号,产后随访应提高对晚期出血发生的警惕性。鉴于B超在诊断动脉瘤方面的有限性,高度怀疑动脉瘤时应尽快行血管造影明确诊断,及时栓塞处理。