Robust cytosolic protein delivery requires both efficient protein binding with delivery vehicles and effective protein release after cell internalization.Although a variety of stimuli-responsive carriers have been des...Robust cytosolic protein delivery requires both efficient protein binding with delivery vehicles and effective protein release after cell internalization.Although a variety of stimuli-responsive carriers have been designed,simultaneously integrating these two functions in one versatile carrier is challenging.Herein,we developed a polyamidoamine(PAMAM)-based polymer with an intracellular pHactuated hydrophobic-to-hydrophilic transition for this purpose.展开更多
基金supported by National Key R&D Program of China(no.2017YFA0205600)Guangdong Natural Science Funds for Distinguished Young Scholar(no.2017A030306018)+4 种基金National Natural Science Foundation of China(nos.51922043 and 31771091)Guangdong Provincial Programs(nos.2017ZT07S054 and 2017GC010304)Outstanding Scholar Program of Guangzhou Regenerative Medicine and Health Guangdong Laboratory(no.2018GZR110102001)the Science and Technology Program of Guangzhou(nos.201902020018 and 201804020060)Fundamental Research Funds for Central Universities.
文摘Robust cytosolic protein delivery requires both efficient protein binding with delivery vehicles and effective protein release after cell internalization.Although a variety of stimuli-responsive carriers have been designed,simultaneously integrating these two functions in one versatile carrier is challenging.Herein,we developed a polyamidoamine(PAMAM)-based polymer with an intracellular pHactuated hydrophobic-to-hydrophilic transition for this purpose.
