Dissolved organic matter(DOM)in refinery wastewater is an extremely complex mixture of various organic compounds.Using mass spectrometry,it is impossible to characterize all of the DOM molecules with only one ionizati...Dissolved organic matter(DOM)in refinery wastewater is an extremely complex mixture of various organic compounds.Using mass spectrometry,it is impossible to characterize all of the DOM molecules with only one ionization source.In this study,negative-ion,electrospray ionization(ESI),positive-ion ESI,and positive-ion atmospheric pressure photoionization(APPI)were coupled with Fourier transform ion cyclotron resonance mass spectrometry(FT-ICR MS)to analyze the molecular composition of DOM in a refinery wastewater stream during the treatment process.There were obvious differences in the heteroatom composition,number of DOM constituents,and chemical properties in refinery wastewater under the three ionization modes.Acidic CHO and CHOS compounds detected by(+)ESI,basic CHN and CHON compounds detected by(þ)ESI,and hydrocarbons detected by(+)APPI were analyzed to determine the molecular transformations that occurred during treatment.In an anaerobic biological treatment process,acidic CHO and CHOS compounds with a high oxygen content were preferentially removed,and acidic CHO and CHOS compounds with a low oxygen content were produced.In an aerobic biological process,acidic CHO and CHOS compounds with a low oxygen content were preferentially removed,and acidic CHO and CHOS compounds with a high oxygen content were produced.The whole biological treatment process has a poor removal efficiency for CHN and CHON compounds,and hydrocarbons.An activated carbon(AC)adsorption process removed different heteroatom compounds mainly with a low oxygen content for acidic and basic compounds.The transformation mechanism of CHO and CHOS compounds in the biological treatment process was analyzed by the Kendrick mass defect(KMD)theory and a mass difference network analysis.In the anaerobic process,large amounts of oxygenated CHO and CHOS compounds were degraded by decarboxylation,deoxydation,demethoxylation,and dehydration reactions,and converted to lower oxygen content compounds.In the aerobic processes,these low oxygen CHO and CHOS compounds mainly underwent carboxylation and oxidation reactions.This study determined the transformation characteristics and mechanisms of different types of organic compounds in refinery wastewater during the treatment process.The results provide guidance for the design and optimization of technologies for refinery wastewater treatment.展开更多
BACKGROUND An increased amount of Fusobacterium nucleatum(F.nucleatum)is frequently detected in the gastric cancer-associated microbiota of the Taiwan Residents population.F.nucleatum is known to exert cytotoxic effec...BACKGROUND An increased amount of Fusobacterium nucleatum(F.nucleatum)is frequently detected in the gastric cancer-associated microbiota of the Taiwan Residents population.F.nucleatum is known to exert cytotoxic effects and play a role in the progression of colorectal cancer,though the impact of F.nucleatum colonization on gastric cancer cells and patient prognosis has not yet been examined.AIM To identify F.nucleatum-dependent molecular pathways in gastric cancer cells and to determine the impact of F.nucleatum on survival in gastric cancer.METHODS Coculture of F.nucleatum with a gastric cancer cell line was performed,and changes in gene expression were investigated.Genes with significant changes in expression were identified by RNA sequencing.Pathway analysis was carried out to determine deregulated cellular functions.A cohort of gastric cancer patients undergoing gastrectomy was recruited,and nested polymerase chain reaction was performed to detect the presence of F.nucleatum in resected cancer tissues.Statistical analysis was performed to determine whether F.nucleatum colonization affects patient survival.RESULTS RNA sequencing and subsequent pathway analysis revealed a drastic interferon response induced by a high colonization load.This response peaked within 24 h and subsided after 72 h of incubation.In contrast,deregulation of actin and its regulators was observed during prolonged incubation under a low colonization load,likely altering the mobility of gastric cancer cells.According to the clinical specimen analysis,approximately one-third of the gastric cancer patients were positive for F.nucleatum,and statistical analysis indicated that the risk for colonization increases in late-stage cancer patients.Survival analysis demonstrated that F.nucleatum colonization was associated with poorer outcomes among patients also positive for Helicobacter pylori(H.pylori).CONCLUSION F.nucleatum colonization leads to deregulation of actin dynamics and likely changes cancer cell mobility.Cohort analysis demonstrated that F.nucleatum colonization leads to poorer prognosis in H.pylori-positive patients with late-stage gastric cancer.Hence,combined colonization of F.nucleatum and H.pylori is a predictive biomarker for poorer survival in late-stage gastric cancer patients treated with gastrectomy.展开更多
The video surveillance systems of recent years, usually major focus on the Human-Face of observation and detection. Human-Face is the most characteristic and prominent feature of a human, therefore, detection and trac...The video surveillance systems of recent years, usually major focus on the Human-Face of observation and detection. Human-Face is the most characteristic and prominent feature of a human, therefore, detection and tracking of Human-Face has become an important indicator of the study. This paper discusses video surveillance of public places and majors in?automated face detection and face tracking. The main detection method is the use of Haar-Like Feature-based and through the Cascade classifier of the Adaboost face detection. In the tracking mechanism is based on particle filter and we modified SURF (Speeded Up Robust Features) particle filter tracking, and thus enhance the detection and tracking accuracy.展开更多
With the development of the bike-sharing system(BSS)and the introduction of green and low carbon development,the environmental impacts of BSS had received increasing attention in recent years.However,the emissions fro...With the development of the bike-sharing system(BSS)and the introduction of green and low carbon development,the environmental impacts of BSS had received increasing attention in recent years.However,the emissions from the rebalancing of BSS,where fossil-fueled vehicles are commonly used,are usually neglected,which goes against the idea of green travel in a sharing economy.Previous studies on the bike-sharing rebalancing problem(BRP),which is considered NP-hard,have mainly focused on algorithm innovation instead of improving the solution model,thereby hindering the application of many existing models in large-scale BRP.This study then proposes a method for optimizing the CO_(2)emissions from BRP and takes the BSS of Beijing as a demonstration.We initially analyze the spatial and temporal characteristics of BSS,especially the flow between districts,and find that each district can be independently rebalanced.Afterward,we develop a rebalancing optimization model based on a partitioning strategy to avoid deciding the number of bikes being loaded or unloaded at each parking node.We then employ the tabu search algorithm to solve the model.Results show that(i)due to over launch and lack of planning in rebalancing,the BSS in Beijing shows great potential for optimization,such as by reducing the number of vehicle routes,CO_(2)emissions,and unmet demands;(ii)the CO_(2)emissions of BSS in Beijing can be reduced by 57.5%by forming balanced parking nodes at the end of the day and decreasing the repetition of vehicle routes and the loads of vehicles;and(iii)the launch amounts of bikes in specific districts,such as Shijingshan and Mentougou,should be increased.展开更多
基金This work was supported by the National Key Research and Development Program of China(2018YFA0605800 and 2020YFA0607600)the National Natural Science Foundation of China(42003059)Science Foundation of China University of Petroleum,Beijing(No.2462021XKBH005).
文摘Dissolved organic matter(DOM)in refinery wastewater is an extremely complex mixture of various organic compounds.Using mass spectrometry,it is impossible to characterize all of the DOM molecules with only one ionization source.In this study,negative-ion,electrospray ionization(ESI),positive-ion ESI,and positive-ion atmospheric pressure photoionization(APPI)were coupled with Fourier transform ion cyclotron resonance mass spectrometry(FT-ICR MS)to analyze the molecular composition of DOM in a refinery wastewater stream during the treatment process.There were obvious differences in the heteroatom composition,number of DOM constituents,and chemical properties in refinery wastewater under the three ionization modes.Acidic CHO and CHOS compounds detected by(+)ESI,basic CHN and CHON compounds detected by(þ)ESI,and hydrocarbons detected by(+)APPI were analyzed to determine the molecular transformations that occurred during treatment.In an anaerobic biological treatment process,acidic CHO and CHOS compounds with a high oxygen content were preferentially removed,and acidic CHO and CHOS compounds with a low oxygen content were produced.In an aerobic biological process,acidic CHO and CHOS compounds with a low oxygen content were preferentially removed,and acidic CHO and CHOS compounds with a high oxygen content were produced.The whole biological treatment process has a poor removal efficiency for CHN and CHON compounds,and hydrocarbons.An activated carbon(AC)adsorption process removed different heteroatom compounds mainly with a low oxygen content for acidic and basic compounds.The transformation mechanism of CHO and CHOS compounds in the biological treatment process was analyzed by the Kendrick mass defect(KMD)theory and a mass difference network analysis.In the anaerobic process,large amounts of oxygenated CHO and CHOS compounds were degraded by decarboxylation,deoxydation,demethoxylation,and dehydration reactions,and converted to lower oxygen content compounds.In the aerobic processes,these low oxygen CHO and CHOS compounds mainly underwent carboxylation and oxidation reactions.This study determined the transformation characteristics and mechanisms of different types of organic compounds in refinery wastewater during the treatment process.The results provide guidance for the design and optimization of technologies for refinery wastewater treatment.
基金by Chiayi Chang Gung Memorial Hospital grants,including No.CORPG6G0021,No.CORPG6G0022 and No.CORPG6G0023 to Wu CSNo.CORPG6G0061,No.CORPG6G0062 and No.CORPG6G0063 to Tung SYNo.CORPG6G0071,No.CORPG6G0072 and No.CORPG6G0073 to Hsieh YY.
文摘BACKGROUND An increased amount of Fusobacterium nucleatum(F.nucleatum)is frequently detected in the gastric cancer-associated microbiota of the Taiwan Residents population.F.nucleatum is known to exert cytotoxic effects and play a role in the progression of colorectal cancer,though the impact of F.nucleatum colonization on gastric cancer cells and patient prognosis has not yet been examined.AIM To identify F.nucleatum-dependent molecular pathways in gastric cancer cells and to determine the impact of F.nucleatum on survival in gastric cancer.METHODS Coculture of F.nucleatum with a gastric cancer cell line was performed,and changes in gene expression were investigated.Genes with significant changes in expression were identified by RNA sequencing.Pathway analysis was carried out to determine deregulated cellular functions.A cohort of gastric cancer patients undergoing gastrectomy was recruited,and nested polymerase chain reaction was performed to detect the presence of F.nucleatum in resected cancer tissues.Statistical analysis was performed to determine whether F.nucleatum colonization affects patient survival.RESULTS RNA sequencing and subsequent pathway analysis revealed a drastic interferon response induced by a high colonization load.This response peaked within 24 h and subsided after 72 h of incubation.In contrast,deregulation of actin and its regulators was observed during prolonged incubation under a low colonization load,likely altering the mobility of gastric cancer cells.According to the clinical specimen analysis,approximately one-third of the gastric cancer patients were positive for F.nucleatum,and statistical analysis indicated that the risk for colonization increases in late-stage cancer patients.Survival analysis demonstrated that F.nucleatum colonization was associated with poorer outcomes among patients also positive for Helicobacter pylori(H.pylori).CONCLUSION F.nucleatum colonization leads to deregulation of actin dynamics and likely changes cancer cell mobility.Cohort analysis demonstrated that F.nucleatum colonization leads to poorer prognosis in H.pylori-positive patients with late-stage gastric cancer.Hence,combined colonization of F.nucleatum and H.pylori is a predictive biomarker for poorer survival in late-stage gastric cancer patients treated with gastrectomy.
文摘The video surveillance systems of recent years, usually major focus on the Human-Face of observation and detection. Human-Face is the most characteristic and prominent feature of a human, therefore, detection and tracking of Human-Face has become an important indicator of the study. This paper discusses video surveillance of public places and majors in?automated face detection and face tracking. The main detection method is the use of Haar-Like Feature-based and through the Cascade classifier of the Adaboost face detection. In the tracking mechanism is based on particle filter and we modified SURF (Speeded Up Robust Features) particle filter tracking, and thus enhance the detection and tracking accuracy.
基金This work was supported by the National Natural Science Foundation of China(Grant Nos.71871022,71828401 and 71521002)the Joint Development Program of Beijing Municipal Commission of Education,the Fok Ying Tung Education Foundation(Grant No.161076)+1 种基金the National Key R&D Program(Grant No.2016YFA0602603)the National Program for Support of Top-notch Young Professionals.
文摘With the development of the bike-sharing system(BSS)and the introduction of green and low carbon development,the environmental impacts of BSS had received increasing attention in recent years.However,the emissions from the rebalancing of BSS,where fossil-fueled vehicles are commonly used,are usually neglected,which goes against the idea of green travel in a sharing economy.Previous studies on the bike-sharing rebalancing problem(BRP),which is considered NP-hard,have mainly focused on algorithm innovation instead of improving the solution model,thereby hindering the application of many existing models in large-scale BRP.This study then proposes a method for optimizing the CO_(2)emissions from BRP and takes the BSS of Beijing as a demonstration.We initially analyze the spatial and temporal characteristics of BSS,especially the flow between districts,and find that each district can be independently rebalanced.Afterward,we develop a rebalancing optimization model based on a partitioning strategy to avoid deciding the number of bikes being loaded or unloaded at each parking node.We then employ the tabu search algorithm to solve the model.Results show that(i)due to over launch and lack of planning in rebalancing,the BSS in Beijing shows great potential for optimization,such as by reducing the number of vehicle routes,CO_(2)emissions,and unmet demands;(ii)the CO_(2)emissions of BSS in Beijing can be reduced by 57.5%by forming balanced parking nodes at the end of the day and decreasing the repetition of vehicle routes and the loads of vehicles;and(iii)the launch amounts of bikes in specific districts,such as Shijingshan and Mentougou,should be increased.
