Living organisms are exposed to the geomagnetic field （GMF） throughout their lifespan. Elimination of the GMF, resulting in a hypogeomagnetic field （HMF）, leads to central nervous system dysfunction and abnormal d...Living organisms are exposed to the geomagnetic field （GMF） throughout their lifespan. Elimination of the GMF, resulting in a hypogeomagnetic field （HMF）, leads to central nervous system dysfunction and abnormal development in animals. However, the cellular mecha-nisms underlying these effects have not been identified so far. Here, we show that exposure to an HMF （〈200 nT）, produced by a magnetic field shielding chamber, pro- motes the proliferation of neural progenitor/stem cells （NPCslNSCs） from C57BL/6 mice. Following seven-day HMF-exposure, the primary neurospheres （NSs） were significantly larger in size, and twice more NPCslNSCs were harvested from neonatal NSs, when compared to the GMF controls. The self-renewal capacity and multi- potency of the NSs were maintalned, as HMF-exposed NSs were posltlve for NSC markers （Nestin and Sox2）, and could differentiate into neurons and astrocytelglial cells and be passaged continuously. In addition, adult mice exposed to the HMF for one month were observed to have a greater number of proliferative cells In the subventrlcular zone. These flndlngs indicate that continuous HMF-exposure increases the proliferation of NPCslNSCs, In vitro and in vlvo. HMF-dlsturbed NPCs/NSCs production probably affects brain development and function, which provides a novel clue for elucidating the cellular mechanisms of the bio.HMF response.展开更多
Accumulative evidence has shown the adverse effects of a geomagnetic field shielded condition, so called a hypomagnetic field （HMF）, on the metabolic processes and oxidative stress in animals and cells. However, the...Accumulative evidence has shown the adverse effects of a geomagnetic field shielded condition, so called a hypomagnetic field （HMF）, on the metabolic processes and oxidative stress in animals and cells. However, the underlying mechanism remains unclear. In this study, we evaluate the role of HMF on the regulation of cellular reactive oxygen species （ROS） in human neuroblastoma SH-SY5Y cells. We found that HMF exposure led to ROS decrease, and that restoring the decrease by additional H2O2 rescued the HMF-enhanced cell proliferation. The measurements on ROS related indexes, including total anti-oxidant capacity, H2O2 and superoxide anion levels, and superoxide dismutase （SOD） activity and expres- sion, indicated that the HMF reduced H2O2 production and inhibited the activity of CuZn-SOD. Moreover, the HMF accelerated the denaturation of CuZn-SOD as well as enhanced aggregation of CuZn-SOD protein, in vitro. Our findings indicate that CuZn-SOD is able to response to the HMF stress and suggest it a mediator of the HMF effect.展开更多
文摘Living organisms are exposed to the geomagnetic field （GMF） throughout their lifespan. Elimination of the GMF, resulting in a hypogeomagnetic field （HMF）, leads to central nervous system dysfunction and abnormal development in animals. However, the cellular mecha-nisms underlying these effects have not been identified so far. Here, we show that exposure to an HMF （〈200 nT）, produced by a magnetic field shielding chamber, pro- motes the proliferation of neural progenitor/stem cells （NPCslNSCs） from C57BL/6 mice. Following seven-day HMF-exposure, the primary neurospheres （NSs） were significantly larger in size, and twice more NPCslNSCs were harvested from neonatal NSs, when compared to the GMF controls. The self-renewal capacity and multi- potency of the NSs were maintalned, as HMF-exposed NSs were posltlve for NSC markers （Nestin and Sox2）, and could differentiate into neurons and astrocytelglial cells and be passaged continuously. In addition, adult mice exposed to the HMF for one month were observed to have a greater number of proliferative cells In the subventrlcular zone. These flndlngs indicate that continuous HMF-exposure increases the proliferation of NPCslNSCs, In vitro and in vlvo. HMF-dlsturbed NPCs/NSCs production probably affects brain development and function, which provides a novel clue for elucidating the cellular mechanisms of the bio.HMF response.
文摘Accumulative evidence has shown the adverse effects of a geomagnetic field shielded condition, so called a hypomagnetic field （HMF）, on the metabolic processes and oxidative stress in animals and cells. However, the underlying mechanism remains unclear. In this study, we evaluate the role of HMF on the regulation of cellular reactive oxygen species （ROS） in human neuroblastoma SH-SY5Y cells. We found that HMF exposure led to ROS decrease, and that restoring the decrease by additional H2O2 rescued the HMF-enhanced cell proliferation. The measurements on ROS related indexes, including total anti-oxidant capacity, H2O2 and superoxide anion levels, and superoxide dismutase （SOD） activity and expres- sion, indicated that the HMF reduced H2O2 production and inhibited the activity of CuZn-SOD. Moreover, the HMF accelerated the denaturation of CuZn-SOD as well as enhanced aggregation of CuZn-SOD protein, in vitro. Our findings indicate that CuZn-SOD is able to response to the HMF stress and suggest it a mediator of the HMF effect.
