OBJECTIVE Liver cancer is one of the most common causes of cancer related deaths worldwide,specially,in China.Hinesol,extracted from Atractylodeslance a(Thunb.) DC.has been proved that has anti-cancer effect in leukem...OBJECTIVE Liver cancer is one of the most common causes of cancer related deaths worldwide,specially,in China.Hinesol,extracted from Atractylodeslance a(Thunb.) DC.has been proved that has anti-cancer effect in leukemia in vitro and in vivo.However,it has been not well under.stood in liver cancer cells.METHODS Cell proliferation,apoptosis,cell cycle and invasion were performed to investigate the anti-liver cancer effect of hinesol in SMMC-7721 and LM3 by MTT assay,flow cytometry and scratch assay.Western blot was used to research the potential mechanism.RESULTS We revealed that hinesol suppresses cell proliferation and invasion,prompts population of G1 phase,induces apop.tosis in dose-dependent manner in SMMC-7721 and LM3 cells.Western blot data showed that hinesol could inhibits the expression of cyclin-D1,Bcl-2 and Bax,and inhibited phosphorylation of MEK and ERK,down-regulated the expressions of NF-κB p65 and phosphor-p65 in nucleus.The results indicated that hinesol reduces cell proliferation via arresting cell cycle at G1 phase and induces apoptosis.Further.more,western blot showed that hinesol inhibited phosphorylation of MEK and ERK,down-regulated the expressions of NF-κB p65 and phosphor-p65 in nucleus.CONCLUSION Our results demonstrate that hinesolreduces cell proliferation via arresting cell cycle at G1 phase and induces apoptosis,it has potent anti-cancer effect against liver cancer cells via down-regulation of MEK/ERK and NF-κB pathway,and indicate that hinesol is a potential liver cancer drug for further research.展开更多
A sensitive and accurate method based on ion chromatography was established for determination of five lithium salts in lithium-ion batteries electrolytes. Chromatographic analyses were carried out on an anion exchange...A sensitive and accurate method based on ion chromatography was established for determination of five lithium salts in lithium-ion batteries electrolytes. Chromatographic analyses were carried out on an anion exchange column at flow rate of 1 m L/min. Under the optimal conditions, five target anions（BF4^-,PF6^-, TFSI^-, BOB^-and FSI^-） exhibited satisfactory linearity with a correlation coefficient of 0.9996. The relative standard derivations of the target anions were less than less than 0.94%（n = 7）. The limits of detections were in the range of 0.068–0.29 mg/L with average spiked recoveries ranging from 96.8% to 105.1%.展开更多
文摘OBJECTIVE Liver cancer is one of the most common causes of cancer related deaths worldwide,specially,in China.Hinesol,extracted from Atractylodeslance a(Thunb.) DC.has been proved that has anti-cancer effect in leukemia in vitro and in vivo.However,it has been not well under.stood in liver cancer cells.METHODS Cell proliferation,apoptosis,cell cycle and invasion were performed to investigate the anti-liver cancer effect of hinesol in SMMC-7721 and LM3 by MTT assay,flow cytometry and scratch assay.Western blot was used to research the potential mechanism.RESULTS We revealed that hinesol suppresses cell proliferation and invasion,prompts population of G1 phase,induces apop.tosis in dose-dependent manner in SMMC-7721 and LM3 cells.Western blot data showed that hinesol could inhibits the expression of cyclin-D1,Bcl-2 and Bax,and inhibited phosphorylation of MEK and ERK,down-regulated the expressions of NF-κB p65 and phosphor-p65 in nucleus.The results indicated that hinesol reduces cell proliferation via arresting cell cycle at G1 phase and induces apoptosis.Further.more,western blot showed that hinesol inhibited phosphorylation of MEK and ERK,down-regulated the expressions of NF-κB p65 and phosphor-p65 in nucleus.CONCLUSION Our results demonstrate that hinesolreduces cell proliferation via arresting cell cycle at G1 phase and induces apoptosis,it has potent anti-cancer effect against liver cancer cells via down-regulation of MEK/ERK and NF-κB pathway,and indicate that hinesol is a potential liver cancer drug for further research.
基金supported by Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province (Nos. 2014006, 2014007)National Important Project on Science Instrument (No. 2012YQ09022903)Zhejiang Provincial Natural Science Foundation of China (Nos. LZ16B050001, LY15B050001, LY12B05003, LQ13B050001, Y13B020001)
文摘A sensitive and accurate method based on ion chromatography was established for determination of five lithium salts in lithium-ion batteries electrolytes. Chromatographic analyses were carried out on an anion exchange column at flow rate of 1 m L/min. Under the optimal conditions, five target anions（BF4^-,PF6^-, TFSI^-, BOB^-and FSI^-） exhibited satisfactory linearity with a correlation coefficient of 0.9996. The relative standard derivations of the target anions were less than less than 0.94%（n = 7）. The limits of detections were in the range of 0.068–0.29 mg/L with average spiked recoveries ranging from 96.8% to 105.1%.
