In the current study,caffeic acid was an important metabolite in the highly copper-tolerant plant Elsholtzia splendens.Preparation and purification of caffeic acid were performed on the dried biomass of the plants by ...In the current study,caffeic acid was an important metabolite in the highly copper-tolerant plant Elsholtzia splendens.Preparation and purification of caffeic acid were performed on the dried biomass of the plants by means of sonication/ethanol extraction,followed by purification using a macroporous resin (D101 type) column and silica gel chromatography.The faint-yellow caffeic acid product was yielded with a purity of 98.46%,and it was chemically identified from spectra of Fourier transform infrared spectroscopy (FTIR),proton nuclear magnetic resonance (1 H NMR)/carbon nuclear magnetic resonance (13 C NMR),and electrospray ionization mass spectrometry (ESI-MS).Caffeic acid is a possible product from the post-harvest processing of Elsholtzia splendens biomass.展开更多
Bisphenol A is a common environmental factor and endocrine disruptor that exerts a negative impact on male reproductive ability.By exploring bisphenol A-induced testicular cell death using the Institute of Cancer Rese...Bisphenol A is a common environmental factor and endocrine disruptor that exerts a negative impact on male reproductive ability.By exploring bisphenol A-induced testicular cell death using the Institute of Cancer Research(ICR)mouse model,we found that a ferroptosis phenomenon may exist.Mice were divided into six groups and administered different doses of bisphenol A via intragastric gavage once daily for 45 consecutive days.Serum was then collected to determine the levels of superoxide dismutase and malondialdehyde.Epididymal sperm was also collected for semen analysis,and testicular tissue was collected for ferritin content determination,electron microscope observation of mitochondrial morphology,immunohistochemistry,real-time quantitative polymerase chain reaction,and western blot analysis.Exposure to bisphenol A was found to decrease sperm quality and cause oxidative damage,iron accumulation,and mitochondrial damage in the testes of mice.In addition,bisphenol A was confirmed to affect the expression of the ferroptosis-related genes,glutathione peroxidase 4(GPX4),ferritin heavy chain 1(FTH1),cyclooxygenase 2(COX2),and acyl-CoA synthetase 4(ACSL4)in mouse testicular tissues.Accordingly,we speculate that bisphenol A induces oxidative stress,which leads to the ferroptosis of testicular cells.Overall,the inhibition of ferroptosis may be a potential strategy to reduce male reproductive toxicity caused by bisphenol A.展开更多
基金Project supported by the Zhejiang Provincial Qianjiang Talents for Science and Technology (No.2011R10026)the Education Department of Zhejiang Province (No.Y201016563)+1 种基金the Research Funds from State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering (No.2009490711)the Fundamental Research Funds for the Central Universities,China
文摘In the current study,caffeic acid was an important metabolite in the highly copper-tolerant plant Elsholtzia splendens.Preparation and purification of caffeic acid were performed on the dried biomass of the plants by means of sonication/ethanol extraction,followed by purification using a macroporous resin (D101 type) column and silica gel chromatography.The faint-yellow caffeic acid product was yielded with a purity of 98.46%,and it was chemically identified from spectra of Fourier transform infrared spectroscopy (FTIR),proton nuclear magnetic resonance (1 H NMR)/carbon nuclear magnetic resonance (13 C NMR),and electrospray ionization mass spectrometry (ESI-MS).Caffeic acid is a possible product from the post-harvest processing of Elsholtzia splendens biomass.
基金This work was supported by grant from the National Natural Science Foundation of China(No.8207152171 and No.82205166)。
文摘Bisphenol A is a common environmental factor and endocrine disruptor that exerts a negative impact on male reproductive ability.By exploring bisphenol A-induced testicular cell death using the Institute of Cancer Research(ICR)mouse model,we found that a ferroptosis phenomenon may exist.Mice were divided into six groups and administered different doses of bisphenol A via intragastric gavage once daily for 45 consecutive days.Serum was then collected to determine the levels of superoxide dismutase and malondialdehyde.Epididymal sperm was also collected for semen analysis,and testicular tissue was collected for ferritin content determination,electron microscope observation of mitochondrial morphology,immunohistochemistry,real-time quantitative polymerase chain reaction,and western blot analysis.Exposure to bisphenol A was found to decrease sperm quality and cause oxidative damage,iron accumulation,and mitochondrial damage in the testes of mice.In addition,bisphenol A was confirmed to affect the expression of the ferroptosis-related genes,glutathione peroxidase 4(GPX4),ferritin heavy chain 1(FTH1),cyclooxygenase 2(COX2),and acyl-CoA synthetase 4(ACSL4)in mouse testicular tissues.Accordingly,we speculate that bisphenol A induces oxidative stress,which leads to the ferroptosis of testicular cells.Overall,the inhibition of ferroptosis may be a potential strategy to reduce male reproductive toxicity caused by bisphenol A.
