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Genetic Analysis and Rescue of a Triple-reassortant H3N2 Influenza A Virus Isolated From Swine in Eastern China 被引量:5
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作者 Xian QI Yong-jun JIAO +5 位作者 Hao PAN Lun-biao CUI wei-xing fan Bao-xu HUANG Zhi-yang SHI Hua WANG 《Virologica Sinica》 SCIE CAS CSCD 2009年第1期52-58,共7页
One influenza H3N2 virus, A/swine/Shandong/3/2005 (Sw/SD/3/2005), was isolated from pigs with respiratory disease on a farm in eastern China. Genetic analysis revealed that Sw/SD/3/2005 was a triple-reassortant virus ... One influenza H3N2 virus, A/swine/Shandong/3/2005 (Sw/SD/3/2005), was isolated from pigs with respiratory disease on a farm in eastern China. Genetic analysis revealed that Sw/SD/3/2005 was a triple-reassortant virus with a PB2 gene from human-like H1N1, NS from classical swine H1N1, and the remaining genes from human-like H3N2 virus. These findings further support the concept that swine can serve as reservoir or mixing vessels of influenza virus strains and maintain genetic and antigenic stability of viruses. Furthermore, we have successfully established a reverse genetics system based on eight plasmids and rescued Sw/SD/3/2005 through cell transfection. HI tests and RT-PCR confirmed that the rescued virus maintained the biological properties of the wild type Sw/SD/3/2005. The successful establishment of the reverse genetics system of Sw/SD/3/2005 will enable us to conduct extensive studies of the molecular evolution of H3N2 influenza viruses in swine. 展开更多
关键词 Influenza A virus H3N2 reassortant Genetic analysis Reverse genetics system SWINE
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Molecular Characterization and Drug Susceptibility of Mycobacterium Bovis Isolated from Cattle in Xinjiang,China
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作者 Xi-Chao Ou fang Xu +4 位作者 Yang Zhou Li-Li Tian Qiao-Ying Zeng wei-xing fan Yan-Lin Zhao 《Chinese Medical Journal》 SCIE CAS CSCD 2018年第24期3017-3019,共3页
To the Editor:Bovine tuberculosis (TB)is a zoonotic disease caused by Mycobacterium bovis.It not only seriously influences the raising of livestock but also threatens the health of human beings and other economically ... To the Editor:Bovine tuberculosis (TB)is a zoonotic disease caused by Mycobacterium bovis.It not only seriously influences the raising of livestock but also threatens the health of human beings and other economically important animals.Therefore, the prevention and control of M.boris has great significance for public and animal health.Spoligotyping is the most widely used method for genotyping of M.boris and investigates transmission across different geographical regions.However,its discriminatory power varies widely among countries.Variable number of tandem repeats (VNTR)analysis has emerged as an alternative method for genotyping of bacterial species isolates. 展开更多
关键词 Molecular Characterization Drug Susceptibility MYCOBACTERIUM BOVIS ISOLATED
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