北京市长辛店地区绝经后女性骨量状态与骨代谢相关指标的关系

目的探讨北京市长辛店地区绝经后女性骨量状态与骨代谢相关指标的关系。方法采用横断面研究方法,在长辛店地区纳入符合研究方案的绝经后女性,签署知情同意书后对其进行骨密度及骨代谢相关指标检测。结果共计743例绝经后女性纳入本研究,根据骨密度诊断确定骨量正常95例,骨量减少343例,骨质疏松305例,骨质疏松患病率为41.05%。骨质疏松人群年龄更大,体重指数更低,绝经年龄更低,绝经年限更长(P<0.01)。血清Ⅰ型胶原羧基端肽交联(βcross-linked C-telopeptide of type 1 collagen,β-CTX)、骨钙素(osteocalcin,OST)、碱性磷酸酶(alkaline phosphatase,ALP)、血清Ⅰ型原胶原氨基端肽(procollagen type 1 N-terminal propeptide,P1NP)、血镁(magnesium,Mg)含量均随着骨量减少而增加,组间差异有统计学意义(P<0.01)。此外,β-CTX(OR=9.662)、ALP(OR=1.008)和OST(OR=1.089)含量变化与骨丢失程度成正比(P<0.05)。结论北京市长辛店地区绝经后女性骨质疏松发生率较高,临床中联合检测骨代谢相关指标及骨密度,可能对全面监测骨的健康状态具有一定积极意义。

Artesunate inhibits growth and induces apoptosis in human osteosarcoma HOS cell line in vitro and in vivo

This paper aims to investigate the effects of artesunate (ART) on growth and apoptosis in human osteosarcoma HOS cell line in vitro and in vivo and to explore the possible underlying mechanisms.Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.The induction of apoptosis was detected by light and transmission electron microscopy and flow cytometry.Western blot analysis was used to investigate the related mechanisms.Nude mice were further employed to investigate the antitumour activity of ART in vivo.MTT assay results demonstrated that ART selectively inhibits the growth of HOS cells in a dose-and time-dependent manner.Based on the findings of light and transmission electron microscopy,Hoechst 33258 staining,and fluorescein isothiocyanate (FITC)-annexin V staining,the cytotoxicity of ART in HOS cells occurs through apoptosis.With ART treatment,cytosolic cytochrome c was increased,Bax expression was gradually upregulated,Bcl-2 expression was downregulated,and caspase-9 and caspase-3 were activated.Thus,the intrinsic apoptotic pathway may be involved in ART-induced apoptosis.Cell cycle analysis by flow cytometry indicated that ART may induce cell cycle arrest at G2 /M phase.In nude mice bearing HOS xenograft tumours,ART inhibited tumour growth and regulated the expressions of cleaved caspase-3 and survivin,in agreement with in vitro observations.ART has a selective antitumour activity against human osteosarcoma HOS cells,which may be related to its effects on induction of apoptosis via the intrinsic pathway.The results suggest that ART is a promising candidate for the treatment of osteosarcoma.