Pharmacological monitoring and literature analysis of dagliflozin in patients with hypovolemia

In the present study,we aimed to explore the timing of dosing with dapagliflozin(DAPA)in patients with hypovolemia and the role of the clinical pharmacist in pharmacological monitoring.The clinical pharmacist was involved in the dosing regimen of two patients with hypovolemia using DAPA and advising patients with insufficient blood volume to withhold the use of the drug.They reviewed the relevant literature to provide a theoretical basis for clinicians and the role of clinical pharmacists in pharmacy services.When considering patients with hypovolemia,the clinical pharmacist can promptly identify that DAPA can reduce blood volume and provide rational advice and rationale for the patient‟s medication that is adopted by the clinician,resulting in an individualized dosing regimen for the patient.Clinical pharmacists are advised to pay attention to the dosing adjustments of DAPA when patients are in hypovolemia and to be more alert to the adverse effects that can result from its use.

Determination of free ceftriaxone concentration and its application in predicting lung tissue concentration

The purpose of this study was to establish a method for determining the free concentration of ceftriaxone based on hollow fiber centrifugal ultrafiltration(HFCF-UF)technology in combination with high-performance liquid chromatography(HPLC)for free pharmacokinetic studies and the prediction of ceftriaxone concentrations in lung tissue.This method only required centrifugation for a short time,and the filtrate could be injected directly for HPLC analysis without further treatment.The specificity,linearity,precision and stability of this method were validated for quantification of free ceftriaxone.Under the optimized conditions,the absolute recoveries were more than 92.5%.The intraday and interday precision RSDs were less than 3.6%.Additionally,nonspecific adsorption(NSB)between the analyte and the ultrafiltration membrane was considered.This method was successfully applied to the analysis of the free ceftriaxone concentration in rat plasma and lung tissue.The free ceftriaxone concentration of lung tissue could be predicted by using the linear formula Cfl=Cfp(0.342 x–0.0129)(x:time).This method also provides a reliable alternative for accurate monitoring of the free ceftriaxone concentration in therapeutic drug monitoring(TDM).