Development and evaluation of a quadruple real-time fluorescence-based quantitative reverse transcription polymerase chain reaction assay for detecting Langya, Mojiang, Nipah, and Cedar viruses

The emerging viruses within the genus Henipavirus in the family Paramyxoviridae pose a great threat to public biosafety.To develop a quadruple real-time fluorescence-based quantitative reverse transcription polymerase chain reaction(qRT-PCR)assay is pivotal for the early warning of the potential of zoonotic infectious diseases.Specific primers and probes were designed for the relatively conserved regions based on whole genome sequences of Langya virus(LayV),Mojiang virus(MojV),Nipah virus(NiV),and Cedar virus(CedV),followed by the establishment of a quadruple real-time fluorescence-based qRT-PCR detection method.No cross-reactivity was observed with other viral nucleic acids.The optimal linear detection range for LayV,MojV,NiV,and CedV was 10^(1)-10^(8)copies/μL,and the lower limit of detection was 10 copies/μL.Three different DNA concentrations of LayV,MojV,NiV,and CedV(10^(4),10^(5),and 10^(6)copies/μL)were tested 14 times,achieving good repeatability.The standard deviation of the cycle threshold values for each concentration was<0.5 and the coefficient of variation was<3%.Furthermore,the amplification efficiency of quadruple real-time fluorescence-based qRT-PCR was>90%,and the correlation coefficient was>0.99.The established quadru-ple real-time fluorescence-based qRT-PCR assay for the detection of LayV,MojV,NiV,and CedV exhibits good sensitivity,specificity,and repeatability.Therefore,it can be used to detect Henipavirus and other related clinical specimens.

Preparation and Thermal Properties of Shape-stabilized Paraffin/NPGDMA/BN Composite for Phase Change Energy Storage

of main observation and conclusion Paraffin,as an excellent phase change material(PCM),is limited by the leakage problem and low thermal conductivity.In this research,a novel paraffin/neopentyl glycol dimethacrylate/boron nitride(paraffin/NPGDMA/BN)composite PCM was successfully prepared via a physical mixing process.NPGDMA in paraffin/NPGDMA/BN composite PCM was cured by UV light under the action of photo initiator,which can improve the shape stability to avoid the leakage of paraffin in the process of phase change.By comparing the leakage rate,latent heat,and thermal conductivity,the optimal mass ratio of paraffin and NPGDMA was selected to be 7:3.It was showed that the presence of 5 wt%BN led to increasing in thermal conductivity by up to 59%.The microstructure of the composite PCMs was characterized by SEM,FTIR,and XRD.The composite PCMs can be processed into a variety of shapes by using molds.The paraffin/NPGDMA/BN composite PCM exhibited a significantly improved shape stability and large phase change enthalpy of 123.0 J/g.The leakage rate of paraffin/NPGDMA/BN was much improved after being soaked with petroleum ether.

Cold-chain transportation in the frozen food industry may have caused a recurrence of COVID-19 cases in destination:Successful isolation of SARS-CoV-2 virus from the imported frozen cod package surface

Coronavirus disease 2019(COVID-19)pandemic has spread in 220 countries/regions towreak havoc to human beings around theworld.At present,the second wave of COVID-19 has begun in many European countries.The complete control of COVID-19 is very urgent.Although China quickly brought the virus under control,there have been eight sporadic outbreaks in China since then.Both in Xinfadi of Beijing and Dalian outbreak of COVID-19,environmental swab samples related to imported cold chain food were tested nucleic acid positive for SARS-CoV-2.In this outbreak in Qingdao,we directly isolated SARS-CoV-2 from the cod outer package's surface swab samples.This is the first time worldwide,SARS-CoV-2 were isolated from the imported frozen cod outer package's surface,which showed that imported frozen food industry could import SARS-CoV-2 virus.

Regulations in the United States for cell transplantation clinical trials in neurological diseases

Objective: This study aimed to use a systematic approach to evaluate the current utilization, safety, and effectiveness of cell therapies for neurological diseases in human. And review the present regulations, considering United States(US) as a representative country, for cell transplantation in neurological disease and discuss the challenges facing the field of neurology in the coming decades. Methods: A detailed search was performed in systematic literature reviews of cellular‐based therapies in neurological diseases, using Pub Med, web of science, and clinical trials. Regulations of cell therapy products used for clinical trials were searched from the Food and Drug Administration(FDA) and the National Institutes of Health(NIH).Results: Seven most common types of cell therapies for neurological diseases have been reported to be relatively safe with varying degrees of neurological recovery.And a series of regulations in US for cellular therapy was summarized including preclinical evaluations, sourcing material, stem cell manufacturing and characterization,cell therapy product, and clinical trials. Conclusions: Stem cell‐based therapy holds great promise for a cure of such diseases and will value a growing population of patients. However, regulatory permitting activity of the US in the sphere of stem cells, technologies of regenerative medicine and substitutive cell therapy are selective, theoretical and does not fit the existing norm and rules. Compiled well‐defined regulations to guide the application of stem cell products for clinical trials should be formulated.

Electric field-induced photoluminescence quenching in Pr-doped BNT ceramics across the MPB region

Piezophotonics is a great interesting field of physics that has led to a number of important technologies,such as light source,smart sensors,and mechatronics.In this work,we reported Pr-doped(Bi_(0.5)Na_(0.5))TiO_(3)-based lead-free ceramics with strong red photoluminescence emission and large strain response(d_(33)^(*)=460 pm/V,S=0.32%).The PL emission can be quenched by decreasing the intensity by 93%after electrical polarization(E=50 kV/cm).The local structure and electric field-induced structural changes were systematically investigated to reveal the significant distinction in photoluminescence properties caused by electrical polarization.The results indicated that polarization treatment eliminates the structural inhomogeneities and establishes a long-range ferroelectric tetragonal and rhombohedral distortion.The crystal structure transformed irreversibly from a non-ergodic to a normal ferroelectric state.PL quenching originated from the decreased distortion of octahedral due to the transition from a non-ergodic state to a highly ordered symmetrical structure.Meanwhile,the enlarged domain structure contributed to the photoluminescence quenching effect.Our findings demonstrate that an electric field can be a robust tool for adjusting the photoluminescence property and provide insights into the rela-tionship between the structure and PL properties of BNT-based ceramics under an external stimulus.