Chrysanthemum Fusarium wilt is a soil-borne disease that causes serious economic losses to the chrysanthemum industry.However,the molecular mechanism underlying the response of chrysanthemum WRKY to Fusarium oxysporum...Chrysanthemum Fusarium wilt is a soil-borne disease that causes serious economic losses to the chrysanthemum industry.However,the molecular mechanism underlying the response of chrysanthemum WRKY to Fusarium oxysporum infection remains largely unknown.In this study,we isolated CmWRKY6–1 from chrysanthemum‘Jinba’and identified it as a transcriptional repressor localized in the nucleus via subcellular localization and transcriptional activation assays.We found that CmWRKY6–1 negatively regulated resistance to F.oxysporum and affected reactive oxygen species(ROS)and salicylic acid(SA)pathways using transgenic experiments and transcriptomic analysis.Moreover,CmWRKY6–1 bound to the W-box element on the CmWRKY15-like promoter and inhibited its expression.Additionally,we observed that CmWRKY15-like silencing in chrysanthemum reduced its resistance to F.oxysporum via transgenic experiments.In conclusion,we revealed the mechanism underlying the CmWRKY6–1–CmWRKY15-like cascade response to F.oxysporum infection in chrysanthemum and demonstrated that CmWRKY6–1 and CmWRKY15-like regulates the immune system.展开更多
基金This research was supported by National Natural Science Foundation of China(32072603)China Agriculture Research System(CARS-23-A18)+1 种基金The JBGS Project of Seed Industry Revitalization in Jiangsu Province[JBGS(2021)094]Jiangsu Agriculture Science and Technology Innovation Fund[CX(22)2033].
文摘Chrysanthemum Fusarium wilt is a soil-borne disease that causes serious economic losses to the chrysanthemum industry.However,the molecular mechanism underlying the response of chrysanthemum WRKY to Fusarium oxysporum infection remains largely unknown.In this study,we isolated CmWRKY6–1 from chrysanthemum‘Jinba’and identified it as a transcriptional repressor localized in the nucleus via subcellular localization and transcriptional activation assays.We found that CmWRKY6–1 negatively regulated resistance to F.oxysporum and affected reactive oxygen species(ROS)and salicylic acid(SA)pathways using transgenic experiments and transcriptomic analysis.Moreover,CmWRKY6–1 bound to the W-box element on the CmWRKY15-like promoter and inhibited its expression.Additionally,we observed that CmWRKY15-like silencing in chrysanthemum reduced its resistance to F.oxysporum via transgenic experiments.In conclusion,we revealed the mechanism underlying the CmWRKY6–1–CmWRKY15-like cascade response to F.oxysporum infection in chrysanthemum and demonstrated that CmWRKY6–1 and CmWRKY15-like regulates the immune system.
