Floquet theorem is widely used in the light-driven systems. But many 2 D-materials models under the radiation are investigated with the high-frequency approximation, which may not be suitable for the practical experim...Floquet theorem is widely used in the light-driven systems. But many 2 D-materials models under the radiation are investigated with the high-frequency approximation, which may not be suitable for the practical experiment. In this work,we employ the non-perturbative Floquet method to strictly investigate the photo-induced topological phase transitions and edge states properties of graphene nanoribbons under the light irradiation of different frequencies(including both low and high frequencies). By analyzing the Floquet energy bands of ribbon and bulk graphene, we find the cause of the phase transitions and its relation with edge states. Besides, we also find the size effect of the graphene nanoribbon on the band gap and edge states in the presence of the light.展开更多
Owing to the diversity of consumer’s demand, the traditional Sichuan brocade products have to be innovated in order to survive in a competitive environment. Since the construction of traditional Sichuan brocade was c...Owing to the diversity of consumer’s demand, the traditional Sichuan brocade products have to be innovated in order to survive in a competitive environment. Since the construction of traditional Sichuan brocade was composed of basic-weave, the surface of the fabric shows a regular interweaving planar texture and is difficult to represent a three-dimensional effect. Inspired by embroidery handcraft, this paper attempts to achieve the embroidery-like effect on the fabric through the jacquard process. Based on the multi-backed structure of traditional Sichuan brocade, we adopted the zoned-combination design mode and added extra free-floats interlacing weave in the area where we want to show the embroidered effect and arranged the interlacing points by referring to the feature of the pattern. As a result, designed Sichuan brocades by this method are capable of displaying embroidered effect with high realism and three-dimensionality. This approach improves the artistic effect of the traditional Sichuan brocade and provides a technical reference for further texture design of jacquard fabrics.展开更多
Figures 2(a)and 2(b)in our original paper[1] should be corrected by the following ones.In Figs.2(a)and 2(b),in the region of hω0>5.96γ0(phase A),the Chern number should be corrected to 1,as indicated in the paper...Figures 2(a)and 2(b)in our original paper[1] should be corrected by the following ones.In Figs.2(a)and 2(b),in the region of hω0>5.96γ0(phase A),the Chern number should be corrected to 1,as indicated in the paper and Table 1.展开更多
Disturbance-Free Payload(DFP)spacecraft can meet the requirements of ultra-high attitude pointing accuracy and stability for future space missions.However,as the main control actuators of DFP spacecraft,Linear Non-Con...Disturbance-Free Payload(DFP)spacecraft can meet the requirements of ultra-high attitude pointing accuracy and stability for future space missions.However,as the main control actuators of DFP spacecraft,Linear Non-Contact Lorentz Actuators(LNCLAs)have control output problems with six-degree-of-freedom coupling and nonlinear effects,which will affect the attitude control performance of DFP spacecraft.To solve this problem,a novel concept for Non-Contact Annular Electromagnetic Stabilized Satellite Platform(NCAESSP)is proposed in this study.The concept is centered on replacing the LNCLAs with a non-contact annular electromagnetic actuator to solve the two problems mentioned above.Furthermore,for the different control requirements of the payload module and the support module of the NCAESSP,a high-precision attitude controller based on the robust model matching method and a dual quaternion-based adaptive sliding mode controller are proposed.Additionally,the simulation results verify the feasibility and effectiveness of the proposed approach.展开更多
Reverse electrodialysis(RED),based on ion-selective membranes,is one of the most promising technologies for capturing osmotic energy.As key elements of the RED system,ion-selective membranes must meet the crucial dema...Reverse electrodialysis(RED),based on ion-selective membranes,is one of the most promising technologies for capturing osmotic energy.As key elements of the RED system,ion-selective membranes must meet the crucial demands of mechanical stability,anti-fouling characteristics,easy fabrication,and high power density;however,this still remains a challenge.In this study,we demonstrated a large-scale,mechanically stable,and high-porosity membrane obtained by combining carbon nanomaterials and hyperbranched polyethyleneimine(h-PEI),thereby achieving a high power density of 5.0 W·m−2 with seawater and river water.Carbon nanofibers(CNFs)were subsequently bridged with graphene and h-PEI to strengthen the interaction between the CNFs,reduce the channel size and increase the space charge density,mechanical strength,and toughness.The large-scale and mechanically stable membrane fabricated using the modified CNFs exhibited anion selectivity and high ionic conductivity,thereby achieving a high-performance osmotic energy conversion.Furthermore,the anti-fouling property of the membrane was confirmed by the stability of the osmotic energy conversion in a solution with algae,which can be attributed to the high porosity of carbon nanomaterials.This economic and convenient method for the ion-selective membrane preparation is believed to be promising for large-scale osmotic energy harvesting.展开更多
Plant cells recognize microbial patterns with the plasma-membrane-localized pattern-recognition receptors consisting mainly of receptor kinases(RKs) and receptor-like proteins(RLPs). RKs, such as bacterial flagellin r...Plant cells recognize microbial patterns with the plasma-membrane-localized pattern-recognition receptors consisting mainly of receptor kinases(RKs) and receptor-like proteins(RLPs). RKs, such as bacterial flagellin receptor FLS2, and their downstream signaling components have been studied extensively. However, newly discovered regulatory components of RLP-mediated immune signaling, such as the nlp20 receptor RLP23, await identification. Unlike RKs, RLPs lack a cytoplasmic kinase domain, instead recruiting the receptor-like kinases(RLKs) BAK1 and SOBIR1. SOBIR1 specifically works as an adapter for RLP-mediated immunity. To identify new regulators of RLP-mediated signaling, we looked for SOBIR1-binding proteins(SBPs) in Arabidopsis thaliana using protein immunoprecipitation and mass spectrometry,identifying two G-type lectin RLKs, SBP1 and SBP2, that physically interacted with SOBIR1.SBP1 and SBP2 showed high sequence similarity,were tandemly repeated on chromosome 4, and also interacted with both RLP23 and BAK1. sbp1 sbp2 double mutants obtained via CRISPR-Cas9 gene editing showed severely impaired nlp20-induced reactive oxygen species burst, mitogenactivated protein kinase(MAPK) activation, and defense gene expression, but normal flg22-induced immune responses. We showed that SBP1 regulated nlp20-induced immunity in a kinase activityindependent manner. Furthermore, the nlp20-induced the RLP23–BAK1 interaction, although not the flg22-induced FLS2–BAK1 interaction, was significantly reduced in sbp1 sbp2. This study identified SBPs as new regulatory components in RLP23 receptor complex that may specifically modulate RLP23-mediated immunity by positively regulating the interaction between the RLP23 receptor and the BAK1 co-receptor.展开更多
Chronic hepatitis B virus(HBV)infection is one of the leading causes of hepatocellular carcinoma(HCC).The HBV genome is prone to mutate and several variants are closely related to the malignant transformation of liver...Chronic hepatitis B virus(HBV)infection is one of the leading causes of hepatocellular carcinoma(HCC).The HBV genome is prone to mutate and several variants are closely related to the malignant transformation of liver disease.G1896A mutation(G to A mutation at nucleotide 1896)is one of the most frequently observed mutations in the precore region of HBV,which prevents HBeAg expression and is strongly associated with HCC.However,the mechanisms by which this mutation causes HCC are unclear.Here,we explored the function and molecular mechanisms of the G1896A mutation during HBV-associated HCC.G1896A mutation remarkably enhanced the HBV replication in vitro.Moreover,it increased tumor formation and inhibited apoptosis of hepatoma cells,and decreased the sensitivity of HCC to sorafenib.Mechanistically,the G1896A mutation could activate ERK/MAPK pathway to enhanced sorafenib resistance in HCC cells and augmented cell survival and growth.Collectively,our study demonstrates for the first time that the G1896A mutation has a dual regulatory role in exacerbating HCC severity and sheds some light on the treatment of G1896A mutation-associated HCC patients.展开更多
This study was conducted to evaluate the effects of dietary crude protein(CP)and rumen-protected lysine(RPL)supplementation on lactation performance,amino acid(AA)balance,nitrogen(N)utilization and hindgut microbiota ...This study was conducted to evaluate the effects of dietary crude protein(CP)and rumen-protected lysine(RPL)supplementation on lactation performance,amino acid(AA)balance,nitrogen(N)utilization and hindgut microbiota in dairy cows.Treatments were in a 2×2 factorial arrangement,and the main effects were CP concentration(16%vs.18%)and RPL supplementation(with or without RPL at40 g/cow per day).Forty cows were randomly allocated to 4 groups:low-CP diet(LP),low-CP diet plus RPL(LPL),high-CP diet(HP),high-CP diet plus RPL(HPL).The experiment was conducted for 8 weeks.Results showed that RPL increased the dry matter intake(P<0.01),milk protein yield(P=0.04)and energy corrected milk(P=0.04),and tended to increase milk fat yield(P=0.06)and fat corrected milk(P=0.05).Cows in the HP group tended to have higher milk urea N(P=0.07).Plasma concentrations of Arg,Ile,Lys,Met,Pro,total essential AA and total nonessential AA were increased by RPL(P<0.05).The total essential AA,total nonessential AA and most AA(except Ile,Phe,Gly and Pro)were increased in the HP group(P<0.05).N excretion was increased in the HP group through an increase in urea N excretion(P<0.01)and an upward trend in plasma urea N(P=0.07).In addition,RPL tended to increase milk protein N secretion(P=0.08),milk N(P=0.07)and microbial protein synthesis(P=0.06),and decreased plasma urea N(P<0.001).In the hindgut,the bacterial community were different between the LP and LPL groups(P<0.01).The probiotic abundances of Christensenellaceae_R-7_group and Acinetobacter were increased by RPL(P=0.03 and 0.03,respectively).The pathogenic abundances of Clostridium_sensu_stricto_1(P<0.001)and Turicibacter(P<0.01)were decreased by RPL.In conclusion,supplementing RPL with low dietary CP could balance AA supply and increase milk protein yield,resulting in an improvement in N utilization efficiency,and altered the composition of the hindgut microbiota to favor the lactation performance of dairy cows.展开更多
Dear editor,The pandemic of coronavirus disease-19(COVID-19)has lasted for more than three years,causing lots of extra death and posing a serious threat to public health.This pandemic swept the last global safety isla...Dear editor,The pandemic of coronavirus disease-19(COVID-19)has lasted for more than three years,causing lots of extra death and posing a serious threat to public health.This pandemic swept the last global safety island in a matter of months,coinciding with the switch of zero-COVID policy of China.The causative agent,SARS-CoV-2,spreads globally and then lurks in the population with sporadic outbreaks in local areas.Meanwhile,the sequelae of convalescent COVID-19 patients receded slowly.The pantissue tropism of SARS-CoV-2 results in a wide range of symptoms in patients,including cardiovascular disorders.Cardiac complications occur in 20%–44%of hospitalized patients,which is an independent risk factor for COVID-19 mortality(Ma et al.,2022;Patone et al.,2022).Survivors of acute COVID-19 also have a substantial risk of cardiovascular disease burden lasting up to one year(Xie et al.,2022).展开更多
Sepsis is a life-threatening organ dysfunction characterized by severe systemic inflammatory response to infection.Effective treatment of bacterial sepsis remains a paramount clinical challenge,due to its astonishingl...Sepsis is a life-threatening organ dysfunction characterized by severe systemic inflammatory response to infection.Effective treatment of bacterial sepsis remains a paramount clinical challenge,due to its astonishingly rapid progression and the prevalence of bacterial drug resistance.展开更多
Dear Editor,Previously,the Mendelian phe no types in huma n oocyte maturation arrest,fertilization failure and early embryonic arrest,are largely underestimated.In recent years,"missing"Men delian phe no typ...Dear Editor,Previously,the Mendelian phe no types in huma n oocyte maturation arrest,fertilization failure and early embryonic arrest,are largely underestimated.In recent years,"missing"Men delian phe no types and genes in these processes are beginning to be uncovered by us and others(Huang et al.,2014;Alazami et al..2015;Feng et al.,2016;Xu et al.,2016;Chen et al.,2017;Sang et al.,2019).However,the genetic basis for majority of patients resulting from abnormalities in these phe no types remains to be elucidated.展开更多
An increasing number of U.S.apple growers are now interested in using harvest platforms to increase harvest productivity,expand labor pool,and alleviate tough working conditions.To maximize benefits,functions of thinn...An increasing number of U.S.apple growers are now interested in using harvest platforms to increase harvest productivity,expand labor pool,and alleviate tough working conditions.To maximize benefits,functions of thinning,pruning&training,and infield sorting have been or are to be incorporated into harvest platforms.Though growers are most concerned with economic benefits,few cost-benefit studies had been conducted on different platforms.In the meantime,economic analysis procedure is complex and each analysis is for one specific machine(not for general purposes).No software has been developed as a general and ready-to-use tool for growers and researchers for the platform economic analysis.In this study,platforms,both available on the marketplace and developed in lab as pilot trials,were reviewed.Costs and benefits models were then established,based on which multi-purpose apple harvest platform economic evaluation software(iMPAHP)was developed(capable of evaluating a wide variety of apple harvest platforms).A case study(machine cost of$100000,accommodating 6 workers,processing apple incidence of 10%with 90%sort-out rate,and harvest,thinning,and pruning&training productivity increase by 40%,50%,and 60%,respectively)based on iMPAHP demonstrated that infield sorting,harvest,thinning,and pruning and training accounted for 48.4%,23.9%,14.3%,and 13.4%of the total benefits,respectively.In the case that the platform was in all-four-purpose-application,the net present value(NPV)analysis of a 10-year investment showed a positive return of$60547.However,without infield sorting function,the NPV resulted in a negative value,indicating a loss for the machine investment.Though incorporating the modular infield sorting system certainly increased the overall machine investment by$30000,the benefits outweighed the costs.展开更多
With the development of nanotechnology and materials science,bioinspired nanochannels appeared by mimicking the intelligent functions of biological ion channels.They have attracted a great deal of at-tention in recent...With the development of nanotechnology and materials science,bioinspired nanochannels appeared by mimicking the intelligent functions of biological ion channels.They have attracted a great deal of at-tention in recent years due to their controllable structure and tunable chemical properties.Inspired by the layered microstructure of nacre,2D layered materials as excellent matrix material of nanochannel come into our field of vision.Bionic nanochannels based on 2D materials have the advantages of facile preparation,tunable channel size and length,easy expansion,and modification,etc.Therefore,the 2D layered nanofluid system based on bionic nanochannels from 2D layered materials has great potential in biomimetic microsensors,membrane separations,energy conversion,and so on.In this paper,we focus on the construction and application of bionic nanochannels based on 2D layer materials.First,a basic understanding of nanochannels based on 2D materials is briefly introduced,we also present the property of the 2D materials and construction strategies of bionic nanochannels.Subsequently,the application of these nanochannels in responsive channels and energy conversion is discussed.The unsolved challenges and prospects of 2D materials-based nanochannels are proposed in the end.展开更多
Proximity labeling catalyzed by promiscuous enzymes,such as APEX2,has emerged as a powerful approach to characterize multiprotein complexes and protein-protein interactions.However,current methods depend on the expres...Proximity labeling catalyzed by promiscuous enzymes,such as APEX2,has emerged as a powerful approach to characterize multiprotein complexes and protein-protein interactions.However,current methods depend on the expression of exogenous fusion proteins and cannot be applied to identify proteins surrounding post-translationally modified proteins.To address this limitation,we developed a new method to label proximal proteins of interest by antibody-mediated protein A-ascorbate peroxidase 2(pA-APEX2) labeling(AMAPEX).In this method,a modified protein is bound in situ by a specific antibody,which then tethers a pA-APEX2 fusion protein.Activation of APEX2 labels the nearby proteins with biotin;the biotinylated proteins are then purified using streptavidin beads and identified by mass spectrometry.We demonstrated the utility of this approach by profiling the proximal proteins of histone modifications including H3 K27 me3,H3 K9 me3,H3 K4 me3,H4 K5 ac,and H4 K12 ac,as well as verifying the co-localization of these identified proteins with bait proteins by published ChIP-seq analysis and nucleosome immunoprecipitation.Overall,AMAPEX is an efficient method to identify proteins that are proximal to modified histones.展开更多
Two azobenzene compounds,Disperse Red 1(DR1)and 4-aminoazobenzene(Aazo),were separately linked to poly(acrylic acid)(PAA)side chains to form PAA-DR1 and PAA-Aazo,which were then associated with poly(ethylene oxide)(PE...Two azobenzene compounds,Disperse Red 1(DR1)and 4-aminoazobenzene(Aazo),were separately linked to poly(acrylic acid)(PAA)side chains to form PAA-DR1 and PAA-Aazo,which were then associated with poly(ethylene oxide)(PEO)to produce hydrogen-bonded polymer complex fibers.After UV irradiation,the initial modulus and yielding strength of PAA-Aazo/PEO fiber were both increased tremendously,while the mechanical properties of PAA-DR1/PEO fiber only slightly changed.After drawn and dried in vacuum,PAA-DR1/PEO and PAA-Aazo/PEO fibers with an extended length exhibited a contraction of 20%and 25%under UV irradiation,showing potential for photo-actuation.展开更多
基金supported by the starting foundation of Chongqing University (Grant No. 0233001104429)the National Natural Science Foundation of China (Grant No. 11847301)the Fundamental Research Funds for the Central Universities,China (Grant No. 2020CQJQY-Z003)。
文摘Floquet theorem is widely used in the light-driven systems. But many 2 D-materials models under the radiation are investigated with the high-frequency approximation, which may not be suitable for the practical experiment. In this work,we employ the non-perturbative Floquet method to strictly investigate the photo-induced topological phase transitions and edge states properties of graphene nanoribbons under the light irradiation of different frequencies(including both low and high frequencies). By analyzing the Floquet energy bands of ribbon and bulk graphene, we find the cause of the phase transitions and its relation with edge states. Besides, we also find the size effect of the graphene nanoribbon on the band gap and edge states in the presence of the light.
文摘Owing to the diversity of consumer’s demand, the traditional Sichuan brocade products have to be innovated in order to survive in a competitive environment. Since the construction of traditional Sichuan brocade was composed of basic-weave, the surface of the fabric shows a regular interweaving planar texture and is difficult to represent a three-dimensional effect. Inspired by embroidery handcraft, this paper attempts to achieve the embroidery-like effect on the fabric through the jacquard process. Based on the multi-backed structure of traditional Sichuan brocade, we adopted the zoned-combination design mode and added extra free-floats interlacing weave in the area where we want to show the embroidered effect and arranged the interlacing points by referring to the feature of the pattern. As a result, designed Sichuan brocades by this method are capable of displaying embroidered effect with high realism and three-dimensionality. This approach improves the artistic effect of the traditional Sichuan brocade and provides a technical reference for further texture design of jacquard fabrics.
文摘Figures 2(a)and 2(b)in our original paper[1] should be corrected by the following ones.In Figs.2(a)and 2(b),in the region of hω0>5.96γ0(phase A),the Chern number should be corrected to 1,as indicated in the paper and Table 1.
基金co-supported by the National Natural Science Foundation of China(No.12172168)。
文摘Disturbance-Free Payload(DFP)spacecraft can meet the requirements of ultra-high attitude pointing accuracy and stability for future space missions.However,as the main control actuators of DFP spacecraft,Linear Non-Contact Lorentz Actuators(LNCLAs)have control output problems with six-degree-of-freedom coupling and nonlinear effects,which will affect the attitude control performance of DFP spacecraft.To solve this problem,a novel concept for Non-Contact Annular Electromagnetic Stabilized Satellite Platform(NCAESSP)is proposed in this study.The concept is centered on replacing the LNCLAs with a non-contact annular electromagnetic actuator to solve the two problems mentioned above.Furthermore,for the different control requirements of the payload module and the support module of the NCAESSP,a high-precision attitude controller based on the robust model matching method and a dual quaternion-based adaptive sliding mode controller are proposed.Additionally,the simulation results verify the feasibility and effectiveness of the proposed approach.
基金The work was supported by the National Natural Science Foundation of China(No.22005162)the Natural Science Foundation of Shandong Province(No.ZR2020QE093)the Special Financial Aid to Post-doctor Research Fellow(No.2020T130330).
文摘Reverse electrodialysis(RED),based on ion-selective membranes,is one of the most promising technologies for capturing osmotic energy.As key elements of the RED system,ion-selective membranes must meet the crucial demands of mechanical stability,anti-fouling characteristics,easy fabrication,and high power density;however,this still remains a challenge.In this study,we demonstrated a large-scale,mechanically stable,and high-porosity membrane obtained by combining carbon nanomaterials and hyperbranched polyethyleneimine(h-PEI),thereby achieving a high power density of 5.0 W·m−2 with seawater and river water.Carbon nanofibers(CNFs)were subsequently bridged with graphene and h-PEI to strengthen the interaction between the CNFs,reduce the channel size and increase the space charge density,mechanical strength,and toughness.The large-scale and mechanically stable membrane fabricated using the modified CNFs exhibited anion selectivity and high ionic conductivity,thereby achieving a high-performance osmotic energy conversion.Furthermore,the anti-fouling property of the membrane was confirmed by the stability of the osmotic energy conversion in a solution with algae,which can be attributed to the high porosity of carbon nanomaterials.This economic and convenient method for the ion-selective membrane preparation is believed to be promising for large-scale osmotic energy harvesting.
基金supported by grants from the National Natural Science Foundation of China (32000202, 32270282, 32000200)the open competition program of the top 10 critical priorities of Agricultural Science and Technology Innovation for the 14th Five-Year Plan of Guangdong Province (2022SDZG07)Double First-class Discipline Promotion Project (2021B10564001)。
文摘Plant cells recognize microbial patterns with the plasma-membrane-localized pattern-recognition receptors consisting mainly of receptor kinases(RKs) and receptor-like proteins(RLPs). RKs, such as bacterial flagellin receptor FLS2, and their downstream signaling components have been studied extensively. However, newly discovered regulatory components of RLP-mediated immune signaling, such as the nlp20 receptor RLP23, await identification. Unlike RKs, RLPs lack a cytoplasmic kinase domain, instead recruiting the receptor-like kinases(RLKs) BAK1 and SOBIR1. SOBIR1 specifically works as an adapter for RLP-mediated immunity. To identify new regulators of RLP-mediated signaling, we looked for SOBIR1-binding proteins(SBPs) in Arabidopsis thaliana using protein immunoprecipitation and mass spectrometry,identifying two G-type lectin RLKs, SBP1 and SBP2, that physically interacted with SOBIR1.SBP1 and SBP2 showed high sequence similarity,were tandemly repeated on chromosome 4, and also interacted with both RLP23 and BAK1. sbp1 sbp2 double mutants obtained via CRISPR-Cas9 gene editing showed severely impaired nlp20-induced reactive oxygen species burst, mitogenactivated protein kinase(MAPK) activation, and defense gene expression, but normal flg22-induced immune responses. We showed that SBP1 regulated nlp20-induced immunity in a kinase activityindependent manner. Furthermore, the nlp20-induced the RLP23–BAK1 interaction, although not the flg22-induced FLS2–BAK1 interaction, was significantly reduced in sbp1 sbp2. This study identified SBPs as new regulatory components in RLP23 receptor complex that may specifically modulate RLP23-mediated immunity by positively regulating the interaction between the RLP23 receptor and the BAK1 co-receptor.
基金This work was supported by the S&T Program of Hebei(20372601D)the Natural Science Foundation of Hebei Province,China(H2020206352,C2021206004)+1 种基金the Science and Technology Project of Hebei Education Department(QN2018150,QN2020268),Hebei Medical Science Research Project(20220973)Chinese Medicine Research Program of Hebei Province(2021119).
文摘Chronic hepatitis B virus(HBV)infection is one of the leading causes of hepatocellular carcinoma(HCC).The HBV genome is prone to mutate and several variants are closely related to the malignant transformation of liver disease.G1896A mutation(G to A mutation at nucleotide 1896)is one of the most frequently observed mutations in the precore region of HBV,which prevents HBeAg expression and is strongly associated with HCC.However,the mechanisms by which this mutation causes HCC are unclear.Here,we explored the function and molecular mechanisms of the G1896A mutation during HBV-associated HCC.G1896A mutation remarkably enhanced the HBV replication in vitro.Moreover,it increased tumor formation and inhibited apoptosis of hepatoma cells,and decreased the sensitivity of HCC to sorafenib.Mechanistically,the G1896A mutation could activate ERK/MAPK pathway to enhanced sorafenib resistance in HCC cells and augmented cell survival and growth.Collectively,our study demonstrates for the first time that the G1896A mutation has a dual regulatory role in exacerbating HCC severity and sheds some light on the treatment of G1896A mutation-associated HCC patients.
基金financially supported by National Natural Science Foundation of China (32172742 and 32202688)the Natural Science Foundation of Zhejiang Province (LQ22C170002)+1 种基金the Zhejiang Provincial Science and Technology Plan Project(2022C04038)the Zhejiang Animal Husbandry Industry Technology Project
文摘This study was conducted to evaluate the effects of dietary crude protein(CP)and rumen-protected lysine(RPL)supplementation on lactation performance,amino acid(AA)balance,nitrogen(N)utilization and hindgut microbiota in dairy cows.Treatments were in a 2×2 factorial arrangement,and the main effects were CP concentration(16%vs.18%)and RPL supplementation(with or without RPL at40 g/cow per day).Forty cows were randomly allocated to 4 groups:low-CP diet(LP),low-CP diet plus RPL(LPL),high-CP diet(HP),high-CP diet plus RPL(HPL).The experiment was conducted for 8 weeks.Results showed that RPL increased the dry matter intake(P<0.01),milk protein yield(P=0.04)and energy corrected milk(P=0.04),and tended to increase milk fat yield(P=0.06)and fat corrected milk(P=0.05).Cows in the HP group tended to have higher milk urea N(P=0.07).Plasma concentrations of Arg,Ile,Lys,Met,Pro,total essential AA and total nonessential AA were increased by RPL(P<0.05).The total essential AA,total nonessential AA and most AA(except Ile,Phe,Gly and Pro)were increased in the HP group(P<0.05).N excretion was increased in the HP group through an increase in urea N excretion(P<0.01)and an upward trend in plasma urea N(P=0.07).In addition,RPL tended to increase milk protein N secretion(P=0.08),milk N(P=0.07)and microbial protein synthesis(P=0.06),and decreased plasma urea N(P<0.001).In the hindgut,the bacterial community were different between the LP and LPL groups(P<0.01).The probiotic abundances of Christensenellaceae_R-7_group and Acinetobacter were increased by RPL(P=0.03 and 0.03,respectively).The pathogenic abundances of Clostridium_sensu_stricto_1(P<0.001)and Turicibacter(P<0.01)were decreased by RPL.In conclusion,supplementing RPL with low dietary CP could balance AA supply and increase milk protein yield,resulting in an improvement in N utilization efficiency,and altered the composition of the hindgut microbiota to favor the lactation performance of dairy cows.
文摘Dear editor,The pandemic of coronavirus disease-19(COVID-19)has lasted for more than three years,causing lots of extra death and posing a serious threat to public health.This pandemic swept the last global safety island in a matter of months,coinciding with the switch of zero-COVID policy of China.The causative agent,SARS-CoV-2,spreads globally and then lurks in the population with sporadic outbreaks in local areas.Meanwhile,the sequelae of convalescent COVID-19 patients receded slowly.The pantissue tropism of SARS-CoV-2 results in a wide range of symptoms in patients,including cardiovascular disorders.Cardiac complications occur in 20%–44%of hospitalized patients,which is an independent risk factor for COVID-19 mortality(Ma et al.,2022;Patone et al.,2022).Survivors of acute COVID-19 also have a substantial risk of cardiovascular disease burden lasting up to one year(Xie et al.,2022).
基金This work was supported by the National Natural Science Foundation of China(31500802,82172132,and 82000523)the Natural Science Foundation of Jiangsu Province(BK20190097 and BK20190053)+2 种基金the Program of Qilu Young Scholars of Shandong UniversityThis work was also supported by the Cyrus Tang Foundation,the Taishan Scholars Program for Young Expert of Shandong Province(tsqn201909021)the Youth Cross-Scientific Innovation Group of Shandong University.
文摘Sepsis is a life-threatening organ dysfunction characterized by severe systemic inflammatory response to infection.Effective treatment of bacterial sepsis remains a paramount clinical challenge,due to its astonishingly rapid progression and the prevalence of bacterial drug resistance.
基金This work was supported by Natural Science Foundation of China (60977058), Science Fund for Distinguished Young Scholars of Shandong Province of China (JQ200819), Independent Innovation Foundation of Shandong University (IIFSDU2010JC002&2012JC015), and promotive research fund for excellent young and middle-aged scientists of Shandong Province (BS2010DX028).
文摘Dear Editor,Previously,the Mendelian phe no types in huma n oocyte maturation arrest,fertilization failure and early embryonic arrest,are largely underestimated.In recent years,"missing"Men delian phe no types and genes in these processes are beginning to be uncovered by us and others(Huang et al.,2014;Alazami et al..2015;Feng et al.,2016;Xu et al.,2016;Chen et al.,2017;Sang et al.,2019).However,the genetic basis for majority of patients resulting from abnormalities in these phe no types remains to be elucidated.
基金This research was funded by the Youth Innovation Promotion Association of Chinese Academy of Sciences(1110000002)the Key Project of Chinese Academy of Sciences(QYZDJ-SSWDQC040).
文摘An increasing number of U.S.apple growers are now interested in using harvest platforms to increase harvest productivity,expand labor pool,and alleviate tough working conditions.To maximize benefits,functions of thinning,pruning&training,and infield sorting have been or are to be incorporated into harvest platforms.Though growers are most concerned with economic benefits,few cost-benefit studies had been conducted on different platforms.In the meantime,economic analysis procedure is complex and each analysis is for one specific machine(not for general purposes).No software has been developed as a general and ready-to-use tool for growers and researchers for the platform economic analysis.In this study,platforms,both available on the marketplace and developed in lab as pilot trials,were reviewed.Costs and benefits models were then established,based on which multi-purpose apple harvest platform economic evaluation software(iMPAHP)was developed(capable of evaluating a wide variety of apple harvest platforms).A case study(machine cost of$100000,accommodating 6 workers,processing apple incidence of 10%with 90%sort-out rate,and harvest,thinning,and pruning&training productivity increase by 40%,50%,and 60%,respectively)based on iMPAHP demonstrated that infield sorting,harvest,thinning,and pruning and training accounted for 48.4%,23.9%,14.3%,and 13.4%of the total benefits,respectively.In the case that the platform was in all-four-purpose-application,the net present value(NPV)analysis of a 10-year investment showed a positive return of$60547.However,without infield sorting function,the NPV resulted in a negative value,indicating a loss for the machine investment.Though incorporating the modular infield sorting system certainly increased the overall machine investment by$30000,the benefits outweighed the costs.
基金supported by the National Natural Science Foundation of China (No. 22005162)the Natural Science Foundation of Shandong Province (No. ZR2020QE093)+1 种基金the China Postdoctoral Science Foundation (No. 2019M652319)the Special Financial Aid to Post-doctor Research Fellow (No. 2020T130330)
文摘With the development of nanotechnology and materials science,bioinspired nanochannels appeared by mimicking the intelligent functions of biological ion channels.They have attracted a great deal of at-tention in recent years due to their controllable structure and tunable chemical properties.Inspired by the layered microstructure of nacre,2D layered materials as excellent matrix material of nanochannel come into our field of vision.Bionic nanochannels based on 2D materials have the advantages of facile preparation,tunable channel size and length,easy expansion,and modification,etc.Therefore,the 2D layered nanofluid system based on bionic nanochannels from 2D layered materials has great potential in biomimetic microsensors,membrane separations,energy conversion,and so on.In this paper,we focus on the construction and application of bionic nanochannels based on 2D layer materials.First,a basic understanding of nanochannels based on 2D materials is briefly introduced,we also present the property of the 2D materials and construction strategies of bionic nanochannels.Subsequently,the application of these nanochannels in responsive channels and energy conversion is discussed.The unsolved challenges and prospects of 2D materials-based nanochannels are proposed in the end.
基金supported by the National Key R&D Program of China(Grant No.2019YFA0903803)the Major Program of National Natural Science Foundation of China(Grant No.32090031)+10 种基金the General Program of National Natural Science Foundation of China(Grant Nos.31971354 and 32070610)the National Natural Science Foundation of China for Young Scholars(Grant No.32000580)the Guangdong Province Fund for Distinguished Young Scholars,China(Grant No.2021B1515020109)the Key Project from Natural Science Foundation of Guangdong Province,China(Grant No.2020B1515120034)the Guangdong Provincial Key Laboratory of Synthetic Genomics,China(Grant No.2019B030301006)the Shenzhen Key Laboratory of Synthetic Genomics,China(Grant No.ZDSYS201802061806209)the Project from Shenzhen Science and Technology Innovation Committee,China(Grant No.JCYJ20170818164014753)the Mayo Clinic Cancer Center Eagles Cancer Fund awarded to ZWthe Mayo Clinic Cancer Center Hematologic Malignancies Program awarded to ZWthe Mayo Clinic division of Hematology awarded to ZWthe Mayo Clinic Center for Biomedical Discovery awarded to SMO,United States。
文摘Proximity labeling catalyzed by promiscuous enzymes,such as APEX2,has emerged as a powerful approach to characterize multiprotein complexes and protein-protein interactions.However,current methods depend on the expression of exogenous fusion proteins and cannot be applied to identify proteins surrounding post-translationally modified proteins.To address this limitation,we developed a new method to label proximal proteins of interest by antibody-mediated protein A-ascorbate peroxidase 2(pA-APEX2) labeling(AMAPEX).In this method,a modified protein is bound in situ by a specific antibody,which then tethers a pA-APEX2 fusion protein.Activation of APEX2 labels the nearby proteins with biotin;the biotinylated proteins are then purified using streptavidin beads and identified by mass spectrometry.We demonstrated the utility of this approach by profiling the proximal proteins of histone modifications including H3 K27 me3,H3 K9 me3,H3 K4 me3,H4 K5 ac,and H4 K12 ac,as well as verifying the co-localization of these identified proteins with bait proteins by published ChIP-seq analysis and nucleosome immunoprecipitation.Overall,AMAPEX is an efficient method to identify proteins that are proximal to modified histones.
基金National Natural Science Foundation of China(No.51973029)Science and Technology Commission of Shanghai Municipality(No.20JC1414900).
文摘Two azobenzene compounds,Disperse Red 1(DR1)and 4-aminoazobenzene(Aazo),were separately linked to poly(acrylic acid)(PAA)side chains to form PAA-DR1 and PAA-Aazo,which were then associated with poly(ethylene oxide)(PEO)to produce hydrogen-bonded polymer complex fibers.After UV irradiation,the initial modulus and yielding strength of PAA-Aazo/PEO fiber were both increased tremendously,while the mechanical properties of PAA-DR1/PEO fiber only slightly changed.After drawn and dried in vacuum,PAA-DR1/PEO and PAA-Aazo/PEO fibers with an extended length exhibited a contraction of 20%and 25%under UV irradiation,showing potential for photo-actuation.