Peri-implant epithelial sealing is the first line of defense against external pathogens or stimuli;hence,an essential process to prevent peri-implantitis.Laminin 332(LN332)is the main component of the internal basal l...Peri-implant epithelial sealing is the first line of defense against external pathogens or stimuli;hence,an essential process to prevent peri-implantitis.Laminin 332(LN332)is the main component of the internal basal lamina and participates in peri-implant epithelial sealing by forming hemidesmosomes(HDs)with integrinα6β4.In thiswork,poly(D,L-lactide)(PDLLA)-LN332 composite coating was successfully constructed by a method similar to layer-by-layer assembly,displaying staged LN332 release for as long as 28days.The PDLLA-LN332 composite coating can activate the intracellular PI3K-Akt pathway via binding to cellular integrinα6β4,which can promote adhesion,migration and proliferation of HaCaT cells and further enhance the expression of keratinocyte HD-related molecules,including integrinα6β4,LN332 and plectin.Furthermore,the PDLLA-LN332 composite coating can promote the adhesion,spreading and proliferation of gingival mesenchymal stem cells and accelerate their epithelial differentiation.Therefore,the PDLLA-LN332 composite coating can enhance implant soft tissue sealing,warranting further in vivo study.展开更多
基金supported by the National Natural Science Foundation of China(No.81970971)Shaanxi Key Research and Development Program(No.2022SF-179).
文摘Peri-implant epithelial sealing is the first line of defense against external pathogens or stimuli;hence,an essential process to prevent peri-implantitis.Laminin 332(LN332)is the main component of the internal basal lamina and participates in peri-implant epithelial sealing by forming hemidesmosomes(HDs)with integrinα6β4.In thiswork,poly(D,L-lactide)(PDLLA)-LN332 composite coating was successfully constructed by a method similar to layer-by-layer assembly,displaying staged LN332 release for as long as 28days.The PDLLA-LN332 composite coating can activate the intracellular PI3K-Akt pathway via binding to cellular integrinα6β4,which can promote adhesion,migration and proliferation of HaCaT cells and further enhance the expression of keratinocyte HD-related molecules,including integrinα6β4,LN332 and plectin.Furthermore,the PDLLA-LN332 composite coating can promote the adhesion,spreading and proliferation of gingival mesenchymal stem cells and accelerate their epithelial differentiation.Therefore,the PDLLA-LN332 composite coating can enhance implant soft tissue sealing,warranting further in vivo study.
