Smartphone-Imaged HIV-1 Reverse-Transcription Loop-Mediated Isothermal Ampliflcation(RT-LAMP) on a Chip from Whole Blood

Viral load measurements are an essential tool for the long-term clinical care of hum an immunodeficiency virus(HIV)-positive individuals. The gol d standards in viral load instrumentation, however, are still too limited by their size, cost, and sophisticated operation for these measurements to be ubiquitous in remote settings with poor healthcare infrastructure, including parts of the world that are disproportionately affected by HIV infection. The challenge of developing a point-of-care platform capable of making viral load more accessible has been frequently approached but no solution has yet emerged that meets the practical requirements of low cost, portability, and ease-of-use. In this paper, we perform reverse-transcription loop-mediated isothermal amplification(RT-LAMP) on minimally processed HIV-spiked whole blood samples with a microfluidic and silicon microchip platform, and perform fluorescence measurements with a consumer smartphone. Our integrated assay shows amplification from as few as three viruses in a ~ 60 nL RTLAMP droplet, corresponding to a whole blood concentration of 670 viruses per μL of whole blood. The technology contains greater power in a digital RT-LAMP approach that could be scaled up for the determination of viral load from a flnger prick of blood in the clinical care of HIV-positive individuals. We demonstrate that all aspects of this viral load approach, from a drop of blood to imaging the RT-LAMP reaction, are compatible with lab-on-a-chip components and mobile instrumentation.

Research on Storage Capacity of Compressed Air Pumped Hydro Energy Storage Equipment

Compressed air pumped hydro energy storage equipment combines compressed air energy storage technology and pumped storage technology. The water is pumped to a vessel to compress air for energy storage, and the compressed air expanses pushing water to drive the hydro turbine for power generation. The novel storage equipment saves natural gas resources, reduces carbon emission, and improves the controllability and reliability. The principle of compressed air pumped hydro energy storage is introduced and its mathematical model is built. The storage and generation process of the novel equipment is analyzed using the model. The calculation formula of the storage power is deduced in theory in different situations of isothermal and adiabatic compression. The optimal storage scheme is given when the capacity and withstand pressure of the vessel is definitive, and the max available capacity and the equipment utilization efficiency evaluation of the scheme is given.

Economic Benefit Analysis of 220 kV Energy-saving Power Transformer

Power transformer serves as one of the most widely used electrical equipments in power grid. During the operation, terrible losses are produced. With the development of loss reduction technology of power transformers, in order to save energy saving and reduce emissions, the old power transformer should be replaced. The paper summarizes the main method to reduce the losses of power transformers and brings up the improved Total Owning Cost (TOC) algorithm, which applies to 220 kV power transformers’ comprehensive benefit analysis. Using the improved Total Owning Cost (TOC) algorithm, based on today 220 kV energy-saving power transformer manufacturing level, the economic benefits of new energy-saving power transformer and the return period of investment are analyzed. Finally, combined with energy-saving effect, the appropriate replacement proposal of 220 kV power transformers has been given.

Research on New Compressed Air Energy Storage Technology

In recent years, wind power generation and photovoltaic power generation have been developing rapidly, and the installed capacity of the new resources generation has been keeping a fast growth every year. But with the incorporation into the grid, the new resources generation that has the properties such as randomness and volatility causes certain risks to the power grid, which results in the falling of the incorporation proportion instead of rising. This paper describes the current status and development problems of the new energy in China, and gives a brief introduction of characteristics of various energy storage technologies. This paper focuses on the analysis of the compressed air energy storage technology in recent years and new developments and the latest technology at home and abroad, additionally, the paper introduces a new concept of the compressed air energy storage system.

Reservoir accumulation conditions and key exploration&development technologies for Keshen gas field in Tarim Basin

The Keshen gas field is located in the central part of Kuqa foreland thrust belt in Tarim Basin,and is another large gas field discovered in Kuqa depression after Kela 2 gas field.Since the breakthrough in 2008,a number of large and medium scale gas reservoirs including Keshen 2,Keshen 5 and Keshen 8 have been discovered,that are characterized by ultra depth,ultra-high pressure,ultra-low porosity,ultra-low permeability,high temperature and high pressure.With natural gas geological reserves of nearly trillion cubic meters and production capacity of nearly 5.5 billion cubic meters,the Keshen gas field is the main natural gas producing area in Tarim Oilfield.The Keshen gas field is located in a series of thrusting imbrication structures in the Kelasu structural belt of Kuqa foreland thrust belt.The salt roof structure,plastic rheology of salt beds and pre-salt faulted anticlinal structure constitute the large wedge-shaped thrust body.The thick delta sandstone of the Cretaceous Bashijike Formation is widely distributed,and it forms the superior reservoir-caprock combination with overlying Paleogene thick gypsum-salt bed.The deep Jurassic-Triassic oil and gas migrate vertically along fault system formed in Late Himalaya,break through the thick Cretaceous mudstone and move laterally along the fracture system of the pre-salt reservoirs,to form anticline and fault anticline high pressure reservoir groups.Through near ten years of studies,the three-dimensional seismic acquisition and processing technology for complex mountainous areas,extrusion salt-related structural modeling technology and fractured low-porosity sandstone reservoir evaluation technology have been established,which lay a foundation for realization of oil and gas exploration objectives.Logging acquisition and evaluation technology for high temperature,high pressure,ultra-deep and low-porosity sandstone gas reservoirs,and efficient development technology for fractured tight sandstone gas reservoirs have been developed,which provide a technical support for efficient exploration&development and rapid production of the Keshen gas field.