Association of age-related hearing loss with psychomotor speed and cognitive function among older adults:a cross-sectional cohort study

To the Editor:Cognitive impairment in older adults is becoming a more prominent issue as the population ages,affecting their social functioning and quality of life.The prevalence of mild cognitive impairment(MCI),as determined in population-based epidemiological studies,ranges from 3%to 19%in adults>65 years.[1]Many risk factors for cognitive decline have been identified,with research identified hearing loss(HL)as an independent risk factor.Age-related hearing loss(ARHL),the third leading cause of chronic disability in older adults,predisposes individuals to cognitive impairment and dementia.

Diversified Application of Barcoded PLATO(PLATO-BC) Platform for Identification of Protein Interactions

Proteins usually associate with other molecules physically to execute their functions.Identifying these interactions is important for the functional analysis of proteins.Previously,we reported the parallel analysis of translated ORFs(PLATO)to couple ribosome display of full-length ORFs with affinity enrichment of mRNA/protein/ribosome complexes for the “bait”molecules,followed by the deep sequencing analysis of mRNA.However,the sample processing,from extraction of precipitated mRNA to generation of DNA libraries,includes numerous steps,which is tedious and may cause the loss of materials.Barcoded PLATO(PLATO-BC),an improved platform was further developed to test its application for protein interaction discovery.In this report,we tested the antisera-antigen interaction using serum samples from patients with inclusion body myositis(IBM).Tripartite motif containing 21(TRIM21)was identified as a potentially new IBM autoantigen.We also expanded the application of PLATO-BC to identify protein interactions for JQ1,single ubiquitin peptide,and NS5 protein of Zika virus.From PLATO-BC analyses,we identified new protein interactions for these “bait”molecules.We demonstrate that Ewing sarcoma breakpoint region 1(EWSR1)binds to JQ1 and their interactions may interrupt the EWSR1 association with acetylated histone H4.RIO kinase 3(RIOK3),a newly identified ubiquitin-binding protein,is preferentially associated with K63-ubiquitin chain.We also find that Zika NS5 protein interacts with two previously unreported host proteins,par-3 family cell polarity regulator(PARD3)and chromosome 19 open reading frame 53(C19orf53),whose attenuated expression benefits the replication of Zika virus.These results further demonstrate that PLATO-BC is capable of identifying novel protein interactions for various types of “bait”molecules.

Controllable synthesis and spontaneous phase transition of photonic coordination polymer to produce a strong second-harmonic generation response

Herein,a novel photonic coordination polymer material was constructed by aggregation-induced emission luminogen(AIEgen)containing a tripyridyl moiety used as the linking ligand.It displayed a spontaneous direct centrosymmetric to noncentrosymmetric phase transition in a single crystal.The two crystals,before and after the phase transition,were both controllably synthesized and characterized by single-crystal X-ray diffraction.After being exposed to air,the centrosymmetric metastable phase(1-α)transitioned to a new stable phase with a noncentrosymmetric structure(1-β).Interestingly,the 1-βstructure exhibited a strong phasematching second-harmonic generation(SHG)response,about4.5 times higher than that of KH2PO4(KDP).In order to better understand the relationship between the structure and the nonlinear optical properties,the dipole moments were calculated and discussed.Remarkably,the noncentrosymmetric phase with high thermal stability for 1-βretained and improved the initial photoluminescent properties of the AIEgen ligand after the structural phase transition from 1-α,and simultaneously produced the excellent SHG property,which are beneficial for the design and construction of excellent optical materials.