Objective To explore the role of MyD88 signaling in MHV-3 virus-mediated fulminant hepatitis. Methods We evaluated liver lesion status, the expression of multiple pro-inflammatory cytokines and HMGB1, the recruitment ...Objective To explore the role of MyD88 signaling in MHV-3 virus-mediated fulminant hepatitis. Methods We evaluated liver lesion status, the expression of multiple pro-inflammatory cytokines and HMGB1, the recruitment of inflammatory ILC3, and mortality in MyD88-/-and WT mice. Results The expression of multiple pro-inflammatory cytokines that recruit inflammatory ILC3 to the liver was severely impaired in MyD88-/-mice resulting in reduced liver pathology, viral replication, and mortality post-infection. Additionally, MHV-3 markedly increased the expression of high-mobility group box 1(HMGB1) in infected hepatocytes/macrophages and induced HMGB1 protein migration from the nucleus to the extracellular milieu, where it activates MyD88-dependent inflammation. Conclusion Our findings indicate that MyD88 exacerbates immunological pathology in experimental viral fulminant hepatitis.展开更多
Particle damping technology has attracted extensive research and engineering application interest in the field of vibration control due to its prominent advantages, including wide working frequency bands, ease of inst...Particle damping technology has attracted extensive research and engineering application interest in the field of vibration control due to its prominent advantages, including wide working frequency bands, ease of installation, longer durability and insensitivity to extreme temperatures. To introduce particle damping technology to long-period structure seismic control, a novel multilayer compartmental particle damper (MCPD) was proposed, and a 1/20 scale test model of a typical long-period self-anchored suspension bridge with a single tower was designed and fabricated. The model was subjected to a series of shaking table tests with and without the MCPD. The results showed that the seismic responses of the flexible or semi-flexible bridge towers of long-period bridges influence the seismic responses of the main beam. The MCPD can be conveniently installed on the main beam and bridge tower and can effectively reduce the longitudinal peak displacement and the root mean square acceleration of the main beam and tower. In addition, no particle accumulation was observed during the tests. A well-designed MCPD can achieve significant damping for long-period structures under seismic excitations of different intensities. These results indicate that the application of MCPDs for seismic control of single-tower self-anchored suspension bridges and other long-period structures is viable.展开更多
Cell-based biosensors (CBBs), a research hot-spot of biosensors, which treat living cells as sensing elements, can detect the functional information of biologically active analytes. They characterize with high sensiti...Cell-based biosensors (CBBs), a research hot-spot of biosensors, which treat living cells as sensing elements, can detect the functional information of biologically active analytes. They characterize with high sensitivity, excellent selectivity and rapid response, and have been applied in many fields, such as biomedicine, environmental monitoring and pharmaceutical screening. Recently cell-cultured technology, silicon microfabrication technology and genetic technology have promoted exploration of CBBs dramatically. To elucidate the novel research findings and applications of cell- based biosensors, this paper summarizes various research approaches, presents some challenges and proposes the research trends.展开更多
基金Supported by grants from the National Natural Sciences Foundation of China(No.81361120400 and 81222023)
文摘Objective To explore the role of MyD88 signaling in MHV-3 virus-mediated fulminant hepatitis. Methods We evaluated liver lesion status, the expression of multiple pro-inflammatory cytokines and HMGB1, the recruitment of inflammatory ILC3, and mortality in MyD88-/-and WT mice. Results The expression of multiple pro-inflammatory cytokines that recruit inflammatory ILC3 to the liver was severely impaired in MyD88-/-mice resulting in reduced liver pathology, viral replication, and mortality post-infection. Additionally, MHV-3 markedly increased the expression of high-mobility group box 1(HMGB1) in infected hepatocytes/macrophages and induced HMGB1 protein migration from the nucleus to the extracellular milieu, where it activates MyD88-dependent inflammation. Conclusion Our findings indicate that MyD88 exacerbates immunological pathology in experimental viral fulminant hepatitis.
基金The research reported in this paper was supported in part by the National Natural Science Foundation of China (Grant No. 51378039)This work was partly supported by the Foundation for Innovative Research Groups of the National Natural Science of China (Grant No. 51421005)+1 种基金This work also was partly supported by the Basic Research Fund of Beijing University of Technology (No. 004000546318524)their support is gratefully acknowledged. The authors thank the anonymous reviewers and the Associate Editor for their constructive comments and advice, which greatly improved the quality of this manuscript.
文摘Particle damping technology has attracted extensive research and engineering application interest in the field of vibration control due to its prominent advantages, including wide working frequency bands, ease of installation, longer durability and insensitivity to extreme temperatures. To introduce particle damping technology to long-period structure seismic control, a novel multilayer compartmental particle damper (MCPD) was proposed, and a 1/20 scale test model of a typical long-period self-anchored suspension bridge with a single tower was designed and fabricated. The model was subjected to a series of shaking table tests with and without the MCPD. The results showed that the seismic responses of the flexible or semi-flexible bridge towers of long-period bridges influence the seismic responses of the main beam. The MCPD can be conveniently installed on the main beam and bridge tower and can effectively reduce the longitudinal peak displacement and the root mean square acceleration of the main beam and tower. In addition, no particle accumulation was observed during the tests. A well-designed MCPD can achieve significant damping for long-period structures under seismic excitations of different intensities. These results indicate that the application of MCPDs for seismic control of single-tower self-anchored suspension bridges and other long-period structures is viable.
基金This work was supported by the Foundation for University Key Teachers by the Ministry of Education (Grant No. 2000-1787)Key Project of National Science and Technology Ministry of China (Grant No. 99-929-04-03)the High-tech Project of the National
文摘Cell-based biosensors (CBBs), a research hot-spot of biosensors, which treat living cells as sensing elements, can detect the functional information of biologically active analytes. They characterize with high sensitivity, excellent selectivity and rapid response, and have been applied in many fields, such as biomedicine, environmental monitoring and pharmaceutical screening. Recently cell-cultured technology, silicon microfabrication technology and genetic technology have promoted exploration of CBBs dramatically. To elucidate the novel research findings and applications of cell- based biosensors, this paper summarizes various research approaches, presents some challenges and proposes the research trends.
