A bacterial strain WY047 was isolated from fermented grains and the bacterium was identified as Bacillus amyloliquefaciens, based on morphological, biochemical, and physiological tests, and analysis of 16S rRNA and gy...A bacterial strain WY047 was isolated from fermented grains and the bacterium was identified as Bacillus amyloliquefaciens, based on morphological, biochemical, and physiological tests, and analysis of 16S rRNA and gyr A sequences. The culture supernatant of WY047 demonstrated inhibition ofa wide spectrum ofbacteria(Gram positive and Gram negative) and fungi. Nine pairs ofprimers were designed and six genes( bmyD, fenA, hag, ituA, mrsA, and tasA) ofantimicrobial substances were detected by PCR, one ofwhich was isolated by 80% ammonium sulfate precipitation, D201 resin anion-exchange chromatography, and Sephadex G-75 filtration column. The purified peptide was estimated to be 35,207 Da and identified as flagellin by MALDI-TOF mass spectrometry. Another four antimicrobial substances were extracted with methanol and identified as iturin A, fengycin, bacillomycin D, and mersacidin through the liquid chromatography–mass spectrometry(LC–MS) method. The sixth possible peptide encoded by tas A could not be isolated in this study; however, the broader spectrum suggested huge application prospects.展开更多
Drought is a major environmental factor that limits the yield of rice dramatically.Upland rice is now regarded as a promising rice cultivar in water saving agriculture.Two varieties of upland rice Zhonghan 3 and IR29 ...Drought is a major environmental factor that limits the yield of rice dramatically.Upland rice is now regarded as a promising rice cultivar in water saving agriculture.Two varieties of upland rice Zhonghan 3 and IR29 were used to compare the physiological and proteomic responses to hyper-osmotic stress induced by 15%polyethyleneglycol(PEG)at the reproductive stage.Osmotic stress affected the growth development and caused the loss of production especially the grain yield.IR29 was more tolerant to PEG than Zhonghan 3 as shown by less yield loss under osmotic stress conditions.Comparative proteomic analysis of the panicle suggested that the up-regulation of glycolysis related proteins and defense proteins may contribute to the better osmotic tolerance in IR29.展开更多
文摘A bacterial strain WY047 was isolated from fermented grains and the bacterium was identified as Bacillus amyloliquefaciens, based on morphological, biochemical, and physiological tests, and analysis of 16S rRNA and gyr A sequences. The culture supernatant of WY047 demonstrated inhibition ofa wide spectrum ofbacteria(Gram positive and Gram negative) and fungi. Nine pairs ofprimers were designed and six genes( bmyD, fenA, hag, ituA, mrsA, and tasA) ofantimicrobial substances were detected by PCR, one ofwhich was isolated by 80% ammonium sulfate precipitation, D201 resin anion-exchange chromatography, and Sephadex G-75 filtration column. The purified peptide was estimated to be 35,207 Da and identified as flagellin by MALDI-TOF mass spectrometry. Another four antimicrobial substances were extracted with methanol and identified as iturin A, fengycin, bacillomycin D, and mersacidin through the liquid chromatography–mass spectrometry(LC–MS) method. The sixth possible peptide encoded by tas A could not be isolated in this study; however, the broader spectrum suggested huge application prospects.
基金supported by the National High Technology Research and Development Program of China(No.2007AA100603)the International Science&Technology Cooperation Program of China(No.2010DFA91930)a project from the Ministry of Agriculture of China for transgenic research(No.2008ZX08009).
文摘Drought is a major environmental factor that limits the yield of rice dramatically.Upland rice is now regarded as a promising rice cultivar in water saving agriculture.Two varieties of upland rice Zhonghan 3 and IR29 were used to compare the physiological and proteomic responses to hyper-osmotic stress induced by 15%polyethyleneglycol(PEG)at the reproductive stage.Osmotic stress affected the growth development and caused the loss of production especially the grain yield.IR29 was more tolerant to PEG than Zhonghan 3 as shown by less yield loss under osmotic stress conditions.Comparative proteomic analysis of the panicle suggested that the up-regulation of glycolysis related proteins and defense proteins may contribute to the better osmotic tolerance in IR29.
