Pollen pollution and allergy are becoming prominent issues in China.However,few studies on pollinosis have been reported.As an allergen in the atmosphere,allergenic Humulus scandens pollen was collected from four dist...Pollen pollution and allergy are becoming prominent issues in China.However,few studies on pollinosis have been reported.As an allergen in the atmosphere,allergenic Humulus scandens pollen was collected from four districts of Shanghai,including Wusong(WS),Jiading(KD),Xujiahui(XJH) and Songjiang(SJ).The mass concentrations of SO2,NO2,O3,PM10,and PM2.5(particulate matter with air dynamic diameter less than 10 and 2.5 um,respectively) near the four sampling sites were also recorded during Humulus scandens pollen season.The allergenicity of the Humulus scandens pollen was assessed by using of a rat model and enzyme linked immunosorbent assay(ELISA).Relationships between the allergenicity and air pollutants were correlated.Our results demonstrated that the biological viability of the pollens collected from the four districts exhibited no significant differences.ELISA and dot blotting results further demonstrated that the serum of sensitized rats exhibited much higher immune-reactive response than that of control groups.Western blotting showed that the 15 KD(1 KD=1000 dalton) proteins of Humulus pollen led to the allergic response.The allergenic intensity of Humulus pollen protein from different samples followed the pattern:WS> JD> XJ> SJ.There was a negative relationship between the allergenicity of Humulus pollens and PM10(R=-0.99)/PM2.5(R=-0.73),and a positive relationship with O3(R=0.92).These data clearly showed that PM10 and PM2.5 could enhance Humulus pollen protein release,and 03 could aggravate the allergenicity of the Humulus pollen.展开更多
Pyrrolizidine alkaloids(PAs)are among the most hepatotoxic natural compounds that are widely distributed throughout the world.Most PAs are metabolically activated to trigger toxicity.Exposure to herbal medicine contai...Pyrrolizidine alkaloids(PAs)are among the most hepatotoxic natural compounds that are widely distributed throughout the world.Most PAs are metabolically activated to trigger toxicity.Exposure to herbal medicine containing PAs and food supplements contaminated by PAs is considered to be one of the two main causes of hepatic sinusoidal obstruction syndrome(HSOS),which is a rare hepatic vascular disease with a high mortality rate.PAs-induced HSOS cases have been reported worldwide.However,there is no clinically effective therapy for PAs-induced HSOS,which is partially because the toxic mechanism is not fully understood.This review focuses on updating the information on the metabolism and the molecular mechanisms of PAs hepatotoxicity,including oxidative stress,apoptosis,and dysfunction of bile acid metabolism,and their interactions.展开更多
Mitochondria energize the inner ear to maintain the cochlear potential createdbythe striavascularis,assist the motility of outer hair cells,perform synaptic processes,and maintain the spontaneous and sound-driven disc...Mitochondria energize the inner ear to maintain the cochlear potential createdbythe striavascularis,assist the motility of outer hair cells,perform synaptic processes,and maintain the spontaneous and sound-driven discharges of the spiral ganglion neurons(SGNs).Mitophagy deficiencies induce the accumulation of damaged organelles and mitochondria in cells and are a primary cause of drug-induced hearing loss.展开更多
基金supported by the Natural Science Founda-tion of China(NSFC,No.21477073)
文摘Pollen pollution and allergy are becoming prominent issues in China.However,few studies on pollinosis have been reported.As an allergen in the atmosphere,allergenic Humulus scandens pollen was collected from four districts of Shanghai,including Wusong(WS),Jiading(KD),Xujiahui(XJH) and Songjiang(SJ).The mass concentrations of SO2,NO2,O3,PM10,and PM2.5(particulate matter with air dynamic diameter less than 10 and 2.5 um,respectively) near the four sampling sites were also recorded during Humulus scandens pollen season.The allergenicity of the Humulus scandens pollen was assessed by using of a rat model and enzyme linked immunosorbent assay(ELISA).Relationships between the allergenicity and air pollutants were correlated.Our results demonstrated that the biological viability of the pollens collected from the four districts exhibited no significant differences.ELISA and dot blotting results further demonstrated that the serum of sensitized rats exhibited much higher immune-reactive response than that of control groups.Western blotting showed that the 15 KD(1 KD=1000 dalton) proteins of Humulus pollen led to the allergic response.The allergenic intensity of Humulus pollen protein from different samples followed the pattern:WS> JD> XJ> SJ.There was a negative relationship between the allergenicity of Humulus pollens and PM10(R=-0.99)/PM2.5(R=-0.73),and a positive relationship with O3(R=0.92).These data clearly showed that PM10 and PM2.5 could enhance Humulus pollen protein release,and 03 could aggravate the allergenicity of the Humulus pollen.
基金supported by the National Natural Science Foundation of China(grant number 81603384)the Shanghai Nature Science Foundation(grant number 16ZR1434200)+2 种基金Shanghai Rising-Star Program(grant number 17QA1403600)Program of Shanghai Academic/Technology Research Leader(grant number 17XD1403500)Programof Shanghai Municipal Commission of Health and Family Planning(grant number ZY(2018e2020)-CCCX-5002).
文摘Pyrrolizidine alkaloids(PAs)are among the most hepatotoxic natural compounds that are widely distributed throughout the world.Most PAs are metabolically activated to trigger toxicity.Exposure to herbal medicine containing PAs and food supplements contaminated by PAs is considered to be one of the two main causes of hepatic sinusoidal obstruction syndrome(HSOS),which is a rare hepatic vascular disease with a high mortality rate.PAs-induced HSOS cases have been reported worldwide.However,there is no clinically effective therapy for PAs-induced HSOS,which is partially because the toxic mechanism is not fully understood.This review focuses on updating the information on the metabolism and the molecular mechanisms of PAs hepatotoxicity,including oxidative stress,apoptosis,and dysfunction of bile acid metabolism,and their interactions.
基金This work was funded by the Project for High-level Talents of Basic and Applied Basic Research(Natural Science)in Hainan(2019RC280)the Key Research and Development Project of Hainan(ZDYF2019055)the Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences(630042019014 and 1630042017017).
基金supported by grants from the National Natural Science Foundation of China(No.82171158,82271177,82271185,82171155)the National Key Research and Development,Project of China(No.2020YFC2008500).
文摘Mitochondria energize the inner ear to maintain the cochlear potential createdbythe striavascularis,assist the motility of outer hair cells,perform synaptic processes,and maintain the spontaneous and sound-driven discharges of the spiral ganglion neurons(SGNs).Mitophagy deficiencies induce the accumulation of damaged organelles and mitochondria in cells and are a primary cause of drug-induced hearing loss.
