After large fresh food chain stores have opened online channels,distribution costs are a key factor affecting consumers'online buying behavior,which affects dual-channel pricing.This paper studies the dual-channel...After large fresh food chain stores have opened online channels,distribution costs are a key factor affecting consumers'online buying behavior,which affects dual-channel pricing.This paper studies the dual-channel pricing strategy of large fresh food chain stores on the premise of dividing the quotation,considering the consumer's acceptance of online channels and the sensitivity to distribution costs.The research found that the optimal pricing of online channels is lower than that of retail channels.The optimal pricing of online channels is positively correlated with the acceptance of online channels,and negatively correlated with the sensitivity of consumer distribution costs.Moreover,after retailers have opened online channels,the market scale has expanded compared with traditional retail channels.Finally,numerical experiments are used to analyze the influence of various influencing factors on retailers'decision-making.展开更多
V79-8 is an abnormal cell line which does not have detectable G1 and G2 phases in its cell cycle.This cell line is derived from V79 cell line which has G1 phase but lacks G2 phase. By using an anti-sense approach, CDK...V79-8 is an abnormal cell line which does not have detectable G1 and G2 phases in its cell cycle.This cell line is derived from V79 cell line which has G1 phase but lacks G2 phase. By using an anti-sense approach, CDK4 gene expression was partially inhibited to find whether CDK4 might contribute to the lack of G1 phase in V79-8 cells. Anti-CDK4 anti-sense plasmid was constructed and used to transfect V79-8 cells. Clones of transfected cells (V79-8-asCDK4) were examined, in comparison with V79-8 cells, to determine its growth curve, cell doubling-time (GT), the level of CDK4 gene expression and the levels of expression of some other growth related genes. V79-8-asCDK4 cells showed a slower growth rate with a doubling time 2.5-h longer than that of V79-8 cells. A flow cytometry (FCM) analysis demonstrated that the 2.5 h increase of the doubling time of V79-8-asCDK4 cells was mainly due to the appearance of G1 phase because its G2+M phase was not significantly different from that of V79-8 cells. The展开更多
文摘After large fresh food chain stores have opened online channels,distribution costs are a key factor affecting consumers'online buying behavior,which affects dual-channel pricing.This paper studies the dual-channel pricing strategy of large fresh food chain stores on the premise of dividing the quotation,considering the consumer's acceptance of online channels and the sensitivity to distribution costs.The research found that the optimal pricing of online channels is lower than that of retail channels.The optimal pricing of online channels is positively correlated with the acceptance of online channels,and negatively correlated with the sensitivity of consumer distribution costs.Moreover,after retailers have opened online channels,the market scale has expanded compared with traditional retail channels.Finally,numerical experiments are used to analyze the influence of various influencing factors on retailers'decision-making.
文摘V79-8 is an abnormal cell line which does not have detectable G1 and G2 phases in its cell cycle.This cell line is derived from V79 cell line which has G1 phase but lacks G2 phase. By using an anti-sense approach, CDK4 gene expression was partially inhibited to find whether CDK4 might contribute to the lack of G1 phase in V79-8 cells. Anti-CDK4 anti-sense plasmid was constructed and used to transfect V79-8 cells. Clones of transfected cells (V79-8-asCDK4) were examined, in comparison with V79-8 cells, to determine its growth curve, cell doubling-time (GT), the level of CDK4 gene expression and the levels of expression of some other growth related genes. V79-8-asCDK4 cells showed a slower growth rate with a doubling time 2.5-h longer than that of V79-8 cells. A flow cytometry (FCM) analysis demonstrated that the 2.5 h increase of the doubling time of V79-8-asCDK4 cells was mainly due to the appearance of G1 phase because its G2+M phase was not significantly different from that of V79-8 cells. The
