Effects of the molluscicide can didate PPU06 on alkaline phosphatase in the golden apple snails determined using a near-infrared fluorescent probe

We constructed a reaction-based near-infrared fluorescent probe(Niap) to specifically identify alkaline phosphatase(ALP) with fast red fluorescence enhancement.Based on the positive concentrationdependent manner between the fluorescent intensity of the Niap and ALP,probe Niap was used to study the ALP enrichment and variation in golden apple snails(Pomacea canaliculata) exposed to the molluscicide candidate PPU06.After treatment with different concentrations of PPU06 over various times,three organs of the surviving snails,liver,stomach and plantaris,were frozen and sectioned for fluorescent imaging experiments.With increased PPU06 concentration,red fluorescence substantially increased in the liver and reached a maximum within 24 h when the PPU06 co ncentration was 0.75 mg/L.No obvious changes in the stomach or foot plantaris were found.It showed PPU06 caused liver injury and stimulated the increase of ALP in the liver of P.canaliculata.This study demonstrates a rapid ALP fluorescent identification method that can be used to study the effects of PPU06 on P.canaliculata.It also provides optical evidence that may aid in the discovery of new chemistry for snail control.

基于数据挖掘分析MTERF3基因在肝细胞癌的表达及预后意义

[目的]线粒体转录终止因子3(mitochondrial transcription termination factor 3,MTERF3)是线粒体基因复制和转录的负调控因子,但目前尚未见其在肝细胞癌中的系统研究。该研究基于数据挖掘分析MTERF3基因在肝细胞癌(hepatocellular carcinoma,HCC)及正常肝组织中的表达情况,并进一步探讨MTERF3基因对肝细胞癌患者预后的影响。[方法]利用Oncomine数据库分析MTERF3基因mRNA在正常肝组织和肝细胞癌组织中的表达变化。通过基因表达谱动态分析(Gene Expression Profiling Interactive Analysis,GEPIA)人体正常组织和其相应的肿瘤组织中MTERF3基因的表达差异。通过c Bioportal分析MTERF3基因在肝细胞癌组织中的突变情况。经MethHC数据库分析肝细胞癌和正常肝组织中MTERF3基因DNA启动子区甲基化水平的差异。利用Onco Lnc数据库对MTERF3基因的表达水平与肝细胞癌患者生存率作Kaplan-Meier生存分析和Log-rank检验。在String-DB数据库中探索在线粒体DNA表达调控过程中与MTERF3基因关系密切的相关基因。[结果]与正常肝组织相比,肝细胞癌组织中MTERF3基因在mRNA水平呈显著高表达(P≤0. 05),MTERF3基因DNA启动子区甲基化水平显著降低(P≤0. 05)。肝细胞癌组织中MTERF3基因存在错义突变,导致其编码蛋白质的243位甲硫氨酸突变为缬氨酸(M243V)。MTERF3基因的表达水平与肝细胞癌患者的总生存时间存在相关性(Logrank P≤0. 05),MTERF3基因高表达的肝细胞癌患者总生存时间显著缩短。MFN1、MFN2、VDAC1、ATG12、UBA52、UBB、UBC、PINK1、SQSTM1、RPS27A等基因与MTERF3基因有明显的相互作用。[结论]大样本数据挖掘能迅速地获取肝细胞癌组织中MTERF3基因表达的相关信息,为深入研究MTERF3基因在肝细胞癌发生发展中的作用机制奠定基础。