Research Progress of Cell Wall Breaking Technology and Its Application in Eucommia ulmoides Male Flower Tea

Eucommia ulmoides male flowers are rich in secondary metabolite,which have anti-tumor,sedative hypnotic,hypotensive,hypolipidemic,anti-fatigue,bacteriostatic,antioxidant and anti-aging effects.The conventional processing of E.ulmoides male flowers leads to the loss of nutrients and active ingredients.In recent years,cell wall breaking technology has been developed and utilized in various fields such as traditional Chinese medicine,good,chemical industry and biology.In order to promote the development of the E.ulmoides industry,the cell wall breaking technology and its characteristics are reviewed,and the application advantages of the cell wall breaking technology in the male flowers of E.ulmoides are discussed,and the prospect of the cell wall breaking of E.ulmoides is proposed in this article.

Performance Analysis of Nested-loop Secondary Linear Doubly-fed Machine Considering End Effects

Nested-loop secondary linear doubly-fed machine(NLS-LDFM) is a novel linear machine evolved from rotary brushless doubly-fed induction machine, which has a good application prospect in linear metro. In order to analyze the performance of NLS-LDFM, the mechanism and action rules of end effects are investigated in this paper. Firstly, the mechanism of static and dynamic end effects is analyzed in aspect of direct coupling, winding asymmetry and transient secondary current. Furthermore, based on the winding theory for short primary linear machines, the machine parameters are established qualitatively considering pulsating magnetic field of NLS-LDFM. Finally, the NLS-LDFM performance analysis is supplemented by the finite element algorithm(FEA) simulation and experiments under different operating conditions.

Multi-layer Quasi Three-dimensional Equivalent Model of Axial-Flux Permanent Magnet Synchronous Machine

Axial-flux permanent magnet synchronous machine(AFPMSM)enjoys the merits of high torque density and high efficiency,which make it one good candidate in the direct-drive application.The AFPMSM is usually analyzed based on the three-dimensional finite element method(3D FEM)due to its three-dimensional magnetic field distribution.However,the 3D FEM suffers large amount of calculation,time-consuming and is not suitable for the optimization of AFPMSM.Addressing this issue,a multi-layer quasi three-dimensional equivalent model of the AFPMSM is investigated in this paper,which could take the end leakage into consideration.Firstly,the multi-layer quasi three-dimensional equivalent model of the AFPMSM with single stator and single rotor is derived in details,including the equivalent processes and conversions of structure dimensions,motion conditions and electromagnetic parameters.Then,to consider the influence of end leakage on the performance,a correction factor is introduced in the multi-layer quasi three-dimensional equivalent model.Finally,the proposed multi-layer quasi three-dimensional equivalent model is verified by the 3D FEM based on an AFPMSM under different structure parameters.It demonstrates that the errors of flux linkage and average torque obtained by the multi-layer quasi three-dimensional equivalent model and 3D FEM are only around 2%although the structure parameters of the AFPMSM are varied.Besides,the computation time of one case based on the multi-layer quasi three-dimensional equivalent model is only 6 min,which is much less than that of the 3D FEM,1.8 h,under the same conditions.Thus,the proposed multi-layer quasi three-dimensional equivalent model could be used to optimize the AFPMSM and much time could be saved by this method compared with the 3D FEM.

The Effect of the Cyp19a1 Gene Methylation Modification on Temperature-dependent Sex Determination of Reeves' Turtle(Mauremys reevesii)

Temperature-dependent sex determination（TSD） is a type of environmental sex determination in which the sex of the embryos depends on the ambient temperature; however,the molecular mechanisms governing this process remain unknown.Aromatase,encoded by the cyp19a1 gene,which converts androgens into estrogens in animals,was considered to be the key gene for TSD.In this study,the 5＇-flanking region of the cyp19a1 gene in Reeves＇ turtle（Mauremys reevesii） was cloned,and the promoter region was identified using the luciferase reporter assay.Then the eggs of Reeves＇ turtle were incubated at different temperatures（26°C： male-biased temperature; 29°C： non-sex-biased temperature and 32°C： female-biased temperature）.During the thermosensitive period,the adrenal kidney gonad complexes（AKG） were sampled.DNA methylation analysis of the AKG samples showed that the promoter region of the cyp19a1 gene was significantly de-methylated in the female-biased temperature regime（P＆lt;0.01）.Quantitative analysis of the cyp19a1 gene and estrogen by q PCR and Elisa assay showed that the expression level of the cyp19a1 gene and estrogen content were both upregulated significantly at the female-biased temperature（P＆lt;0.01）.These results indicated that the de-methylation response of the cyp19a1 gene to incubation temperature,especially at the female-biased temperature,could lead to temperature-specific expression of aromatase and increased estrogen levels,which may further determine gonadal development in Reeves＇ turtle.These findings provide insights into the genetic mechanisms underlying TSD.