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Identification of Genetic Relationship of Hybrid Rice Varieties to Their Parents with PCR Products
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作者 Lu LIU Shujuan HU +3 位作者 Xi CHEN weizheng peng Bin YANG Qingcai ZHAN 《Asian Agricultural Research》 2020年第5期40-41,59,共3页
[Objectives]To establish a simple,rapid and accurate method for identifying the genetic relationship of hybrid rice varieties to their parents.[Methods]Taking F1 hybrids Liangyou 336,Deliangyou Huazhan,and the parents... [Objectives]To establish a simple,rapid and accurate method for identifying the genetic relationship of hybrid rice varieties to their parents.[Methods]Taking F1 hybrids Liangyou 336,Deliangyou Huazhan,and the parents of Liangyou 336,i.e.,C815S(♀)and R336(♂),as experimental materials,the genetic relationship of the hybrid rice varieties to the parental materials was identified by way of PCR amplification with the 48 pairs of SSR primers of Protocol for Identification of Rice Varieties:SSR Marker Method(NY/T 1433-2014).[Results]The genetic relationship of the hybrid rice varieties could be determined by comparing the PCR amplification products of the mixed DNA of the parents and the DNA of the F1 hybrids.[Conclusions]This method not only reduced the number of samples required but also had a good visual effect and high accuracy. 展开更多
关键词 Hybrid rice PARENT SSR primer PCR mixed DNA Genetic relationship
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