The interactions between L-tryptophan（L-Trp） and water-soluble p-sulfonatocalix[4]- arene （SCX4） were investigated by spectrofluorometry. The effect of pH was discussed. The interaction process was estimated from ...The interactions between L-tryptophan（L-Trp） and water-soluble p-sulfonatocalix[4]- arene （SCX4） were investigated by spectrofluorometry. The effect of pH was discussed. The interaction process was estimated from the thermodynamic viewpoint. The SCX4 was found to be able to adjust its conformation to fit the size of L-Trp, and the benzene of L-Trp penetrated into the hydrophobic cavity of SCX4. Meanwhile, the binding mechanism was further elucidated by IHNMR and molecular mechanics calculations.展开更多
The interaction of the novel tetra-carboxylphenyl calix[4]arene （TCPC） with the bovine heart cytochrome c （Cc） was first investigated by fluorescence spectroscopy and molecular modeling methods. The formation of a...The interaction of the novel tetra-carboxylphenyl calix[4]arene （TCPC） with the bovine heart cytochrome c （Cc） was first investigated by fluorescence spectroscopy and molecular modeling methods. The formation of a stable 1：1 complex was monitored by fluorescence titration, and its binding constant is 1.916 ×10^7 L mol^-1. Molecular modeling reveals the recognition mechanism of TCPC to the Cc surface, that is, the electrostatic interaction drives TCPC to the Cc surface, and the van der Waals interaction orientates TCPC parallel to the cleft of Cc.展开更多
基金supported by the National Natural Science Foundation of China(No.20575038).
文摘The interactions between L-tryptophan（L-Trp） and water-soluble p-sulfonatocalix[4]- arene （SCX4） were investigated by spectrofluorometry. The effect of pH was discussed. The interaction process was estimated from the thermodynamic viewpoint. The SCX4 was found to be able to adjust its conformation to fit the size of L-Trp, and the benzene of L-Trp penetrated into the hydrophobic cavity of SCX4. Meanwhile, the binding mechanism was further elucidated by IHNMR and molecular mechanics calculations.
基金supported by the National Natural Science Foundation of China (No.90813018)the Youth Foundation of Shanxi Province (No.2006021009)as well as the Youth Scientific and Technical Foundation of Shanxi University (Nos.2006007 and 2007112).
文摘The interaction of the novel tetra-carboxylphenyl calix[4]arene （TCPC） with the bovine heart cytochrome c （Cc） was first investigated by fluorescence spectroscopy and molecular modeling methods. The formation of a stable 1：1 complex was monitored by fluorescence titration, and its binding constant is 1.916 ×10^7 L mol^-1. Molecular modeling reveals the recognition mechanism of TCPC to the Cc surface, that is, the electrostatic interaction drives TCPC to the Cc surface, and the van der Waals interaction orientates TCPC parallel to the cleft of Cc.
