African swine fever(ASF) is a hemorrhagic disease caused by African swine fever virus(ASFV), which belongs to the Asfarviridae family. ASF has become prevalent in Africa since it was first reported in Kenya in 1921(Ro...African swine fever(ASF) is a hemorrhagic disease caused by African swine fever virus(ASFV), which belongs to the Asfarviridae family. ASF has become prevalent in Africa since it was first reported in Kenya in 1921(Rowlands et al. 2008;Costard et al. 2009). In 1957, it was introduced to Portugal in Europe, after which ASFV rapidly spread to other European countries and has already been eradicated in several countries except Sardinia(Mur et al. 2016). In 2007, ASF was introduced to Georgia and has continued its spread to other countries in Europe(Smietanka et al. 2016;Kolbasov et al. 2018;Garigliany et al. 2019;Linden et al. 2019). In China, an ASF outbreak was first reported on August 3, 2018 in a pig farm in Shenyang, Liaoning Province(Zhou et al. 2018). Since then, the dramatic spread of ASF throughout China has caused huge economic losses to the swine industry(Jiang et al. 2021).展开更多
The nonstructural protein 10（nsp10） of porcine reproductive and respiratory syndrome virus（PRRSV） encodes for helicase which plays a vital role in viral replication. In the present study, a truncated form of nsp10...The nonstructural protein 10（nsp10） of porcine reproductive and respiratory syndrome virus（PRRSV） encodes for helicase which plays a vital role in viral replication. In the present study, a truncated form of nsp10, termed nsp10 a, was found in PRRSV-infected cells and the production of nsp10 a was strain-specific. Mass spectrometric analysis and deletion mutagenesis indicated that nsp10 a may be short of about 70 amino acids in the N terminus of nsp10. Further studies by rescuing recombinant viruses showed that the Glu-69 in nsp10 was the key amino acid for nsp10 a production. Finally, we demonstrated that nsp10 a exerted little influence on the growth kinetics of PRRSV in vitro.展开更多
基金supported by the National Natural Science Foundation of China (31902258)the Scientific Research Staring Foundation for the Doctors from the Science and Technology Department of Liaoning Province, China (2019-BS-204)the Major Project of Science and Technology of Liaoning Province (2020JH1/10200003)。
文摘African swine fever(ASF) is a hemorrhagic disease caused by African swine fever virus(ASFV), which belongs to the Asfarviridae family. ASF has become prevalent in Africa since it was first reported in Kenya in 1921(Rowlands et al. 2008;Costard et al. 2009). In 1957, it was introduced to Portugal in Europe, after which ASFV rapidly spread to other European countries and has already been eradicated in several countries except Sardinia(Mur et al. 2016). In 2007, ASF was introduced to Georgia and has continued its spread to other countries in Europe(Smietanka et al. 2016;Kolbasov et al. 2018;Garigliany et al. 2019;Linden et al. 2019). In China, an ASF outbreak was first reported on August 3, 2018 in a pig farm in Shenyang, Liaoning Province(Zhou et al. 2018). Since then, the dramatic spread of ASF throughout China has caused huge economic losses to the swine industry(Jiang et al. 2021).
基金supported by the National Key Technology R&D Program of China (2015BAD12B01-2)the Major Program of National Natural Science Foundation of China (31490603)the earmarked fund for Modern Agroindustry Technology Research System of China (CARS-36)
文摘The nonstructural protein 10（nsp10） of porcine reproductive and respiratory syndrome virus（PRRSV） encodes for helicase which plays a vital role in viral replication. In the present study, a truncated form of nsp10, termed nsp10 a, was found in PRRSV-infected cells and the production of nsp10 a was strain-specific. Mass spectrometric analysis and deletion mutagenesis indicated that nsp10 a may be short of about 70 amino acids in the N terminus of nsp10. Further studies by rescuing recombinant viruses showed that the Glu-69 in nsp10 was the key amino acid for nsp10 a production. Finally, we demonstrated that nsp10 a exerted little influence on the growth kinetics of PRRSV in vitro.
