The lysyl oxidase （LOX） family encodes the copper-dependent amine oxidases that play a key role in determining the tensile strength and structural integrity of connective tissues by catalyzing the crosslinking of el...The lysyl oxidase （LOX） family encodes the copper-dependent amine oxidases that play a key role in determining the tensile strength and structural integrity of connective tissues by catalyzing the crosslinking of elastin or collagen. Estrogen may upregulate the expression of LOXand lysyl oxidase-like 1 （LOXL1） in the vagina. The objec- tive of this study was to determine the effect of estrogen on the expression of all LOX family genes in the urogenital tissues of accelerated ovarian aging mice and human Ishikawa cells. Mice and Ishikawa cells treated with estradiol （E2） showed increased expression of LOXfamily genes and transforming growth factor IB1 （TGF-β1）. Ishikawa cells treated with TGF-β1 also showed increased expression of LOXfamily genes. The Ishikawa cells were then treated with either E2 plus the TGF-β receptor （TGFBR） inhibitor SB431542 or E2 alone. The expression of LOXfamily genes induced by E2 was reduced in the Ishikawa cells treated with TGFBR inhibitor. Our results showed that E2 increased the ex- prassion of the LOXfamily genes, and suggest that this induction may be mediated by the TGF-β signal pathway. E2 may play a role in regulating the expression of LOXfamily genes.展开更多
基金supported by the National Basic Research Program(973)of China(No.2010CB945002)the Shandong Provincial Natural Science Foundation of China(No.ZR2013CQ041)the Independent Innovation Foundation of Shandong University(No.IIFSDU 2013GN011),China
文摘The lysyl oxidase （LOX） family encodes the copper-dependent amine oxidases that play a key role in determining the tensile strength and structural integrity of connective tissues by catalyzing the crosslinking of elastin or collagen. Estrogen may upregulate the expression of LOXand lysyl oxidase-like 1 （LOXL1） in the vagina. The objec- tive of this study was to determine the effect of estrogen on the expression of all LOX family genes in the urogenital tissues of accelerated ovarian aging mice and human Ishikawa cells. Mice and Ishikawa cells treated with estradiol （E2） showed increased expression of LOXfamily genes and transforming growth factor IB1 （TGF-β1）. Ishikawa cells treated with TGF-β1 also showed increased expression of LOXfamily genes. The Ishikawa cells were then treated with either E2 plus the TGF-β receptor （TGFBR） inhibitor SB431542 or E2 alone. The expression of LOXfamily genes induced by E2 was reduced in the Ishikawa cells treated with TGFBR inhibitor. Our results showed that E2 increased the ex- prassion of the LOXfamily genes, and suggest that this induction may be mediated by the TGF-β signal pathway. E2 may play a role in regulating the expression of LOXfamily genes.
