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胞质分裂阻滞微核自动化分析技术的研究进展 被引量:4
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作者 申相 陈英 +2 位作者 温占波 郑劲林 周正干 《辐射研究与辐射工艺学报》 CAS CSCD 2021年第3期1-8,共8页
在辐射生物剂量估算方面,胞质分裂阻滞微核分析(CB微核法)是除染色体畸变之外被国际公认的好方法。为解决CB微核法人工检测的主观性及其费时费力问题,不少学者正在探索自动化方法来实现双核细胞微核的检测。本文通过分析国内外相关文献,... 在辐射生物剂量估算方面,胞质分裂阻滞微核分析(CB微核法)是除染色体畸变之外被国际公认的好方法。为解决CB微核法人工检测的主观性及其费时费力问题,不少学者正在探索自动化方法来实现双核细胞微核的检测。本文通过分析国内外相关文献,将CB微核自动化分析的研究内容进行梳理和归纳,以全面展现当前CB微核自动化分析的研究现状和特点。在此基础上,预测其未来发展趋势,以期对相关领域的同行提供有益的参考。 展开更多
关键词 胞质分裂阻滞微核 图像自动获取 自动分析算法
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大气PM2.5对大鼠心肌细胞的毒性作用 被引量:8
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作者 焦周光 胡凌飞 +3 位作者 李娜 张柯 温占波 李劲松 《环境科学研究》 EI CAS CSCD 北大核心 2018年第9期1636-1644,共9页
为评估大气PM_(2.5)及其不同组分对心肌细胞H9C2的毒性作用,探讨PM_(2.5)对心血管系统产生毒性作用的关键组分,将前期采集并制备的PM_(2.5)完全颗粒物、PM_(2.5)水溶性组分、PM_(2.5)脂溶性组分和PM_(2.5)单纯颗粒物以不同质量浓度对H9C... 为评估大气PM_(2.5)及其不同组分对心肌细胞H9C2的毒性作用,探讨PM_(2.5)对心血管系统产生毒性作用的关键组分,将前期采集并制备的PM_(2.5)完全颗粒物、PM_(2.5)水溶性组分、PM_(2.5)脂溶性组分和PM_(2.5)单纯颗粒物以不同质量浓度对H9C2细胞染毒.用MTS法在染毒6、10、24、48、72 h后测定细胞活力;根据细胞活力测定结果,选用较低染毒浓度(10μg/m L),用相关试剂盒测定染毒24 h后胞内和上清液中LDH(乳酸脱氢酶)和SOD(超氧化物歧化酶)活力,ELISA及RT-q PCR法测定炎性因子IL-6和TNF-α表达量,AP位点计数法测定细胞DNA损伤情况.结果表明:颗粒物成分(PM_(2.5)完全颗粒物和PM_(2.5)单纯颗粒物)对H9C2细胞表现出强烈的生长抑制作用,50μg/m L及以上染毒浓度组在染毒时间≥24 h时细胞可能已经全部死亡,而可溶性成分(PM_(2.5)水溶性组分和PM_(2.5)脂溶性组分)对H9C2细胞生长表现为极弱或无生长抑制作用,仅400μg/m L的PM_(2.5)脂溶性组分始终对细胞生长表现出抑制作用;各组分样本都在一定程度上造成了H9C2细胞损伤,降低了胞内LDH和SOD活性;PM_(2.5)完全颗粒物和PM_(2.5)脂溶性组分在造成炎性损伤方面的作用较为明显.研究显示,颗粒物组分对H9C2细胞致死作用显著,相对而言,PM_(2.5)完全颗粒物表现出的毒性作用最强且最全面. 展开更多
关键词 PM2.5 心肌细胞 细胞活力 炎性因子
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Biological characteristics of marine bacterium S-9801 strain and its culture conditions of pigment production 被引量:2
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作者 Tian Li He Peiqing +3 位作者 Wu Hongqing wen zhanbo Liu Chenlin Li Guangyou 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2002年第4期541-545,共5页
Strain of Flavobacterium sp.(S-9801),was screened from 207 strains of marine bacteria isolated from the Bohai Sea continental shelf and the Zhujiang Estuary,for its red pigment production.The biological characteristic... Strain of Flavobacterium sp.(S-9801),was screened from 207 strains of marine bacteria isolated from the Bohai Sea continental shelf and the Zhujiang Estuary,for its red pigment production.The biological characteristics of strain S-9801 and culture conditions of pigment production have been checked out in this study. The color of the bacterial colony on 2216E medium was from coccineus to rose bengal. Optimum culture conditions were sodium chloride concentration(g/dm3),10~30;pH,3~8;temperature,25~28℃;tryptone and yeast extract as nitrogen sources and glucose as carbon source. Under optimum conditions,pigment accumulation started after 12 h,reaching a maximum rate of synthesis at 36 h. 展开更多
关键词 Marine bacteria pigment production biological characteristics culture conditions
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Studies on the bioactivity of marine microorganisms using Pyricularia oryzae model
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作者 wen zhanbo PEI Yuehu +3 位作者 TIAN Li GAO Aiguo HONG Xuguang LI Xianchun 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2006年第6期153-160,161,共9页
A total of 2 088 marine microorganisms including 1 392 strains of bacteria and 696 strains of fungi were isolated from diverse marine environment such as the South Pole, the North Pole, and Qingdao tideland, etc. Thro... A total of 2 088 marine microorganisms including 1 392 strains of bacteria and 696 strains of fungi were isolated from diverse marine environment such as the South Pole, the North Pole, and Qingdao tideland, etc. Through a systematic screening process involving the use of Pyricularia oryzae mode, 90 bacteria and 31 fungi strains demonstrating positive bioactivities were identified. The rates of active strains of marine bacteria and marine fungi were 6.5% and 4.5%, respectively. The relationships between the active strains and the sampling locations and hosts were studied. For marine bacteria, the three locations showing the highest rotes of active strains were 8.4% in the Zhujiang Estuary, 7.2% at the North Pole and 6.3% in the Bohai Sea. For marine fungi, the highest rates observed were 7.8% at the South Pole, 7.4% in the South China Sea and 6.8% at the Qingdao tideland. An analysis of the host showed that the three highest rates of active strain for marine bacteria were 8.2% for seawater from the Zhujiang Estuary, 8% for alga from the Qingdao tideland and 7.6% for sea mud from the North Pole. For marine fungi, the highest rates observed were 8.3% for Qingdao actiniae, 7.4% for South China Sea poriferan and 4.5% for soil of Qingdao Suaeda salsa. Four fungi and two bacteria samples were selected for further study because of their high bioactivity. They were found to be active towards several pathogenic microorganisms, and demonstrated stable genetic and thermal characteristics and conservation activities. 展开更多
关键词 marine microorganisms activitve substances Pyricularia oryzae model
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