Cymbidium,which includes approximately 80 species,is one of the most ornamental and cultivated orchid genera.However,a lack of markers and sparse sampling have posed great challenges to resolving the phylogenetic rela...Cymbidium,which includes approximately 80 species,is one of the most ornamental and cultivated orchid genera.However,a lack of markers and sparse sampling have posed great challenges to resolving the phylogenetic relationships within the genus.In the present study,we reconstructed the phylogenetic relationships by utilizing one nuclear DNA(nrITS)and seven plastid genes(rbcL,trnS,trnG,matK,trnL,psbA,and atpI)from 70 species(varieties)in Cymbidium.We also examined the occurrence of phylogenetic conflict between nuclear(nrITS)and plastid loci and investigated how phylogenetic conflict bears on taxonomic classification within the genus.We found that phylogenetic conflict and low support values may be explained by hybridization and a lack of informative characteristics.Our results do not support previous classification of the subgenera and sections within Cymbidium.Discordance between gene trees and network analysis indicate that reticulate evolution occurred in the genus Cymbidium.Overall,our study indicates that Cymbidium has undergone a complex evolution.展开更多
基金funded by The National Key Research and Development Program of China(Nos.2018YFD1000401 and 2018YFD1000400)the Natural Science Foundation of Guangdong Province(No.2017A030312004)the Science and Technology Plan Project of Shenzhen(No.JCYJ20170817151501595).
文摘Cymbidium,which includes approximately 80 species,is one of the most ornamental and cultivated orchid genera.However,a lack of markers and sparse sampling have posed great challenges to resolving the phylogenetic relationships within the genus.In the present study,we reconstructed the phylogenetic relationships by utilizing one nuclear DNA(nrITS)and seven plastid genes(rbcL,trnS,trnG,matK,trnL,psbA,and atpI)from 70 species(varieties)in Cymbidium.We also examined the occurrence of phylogenetic conflict between nuclear(nrITS)and plastid loci and investigated how phylogenetic conflict bears on taxonomic classification within the genus.We found that phylogenetic conflict and low support values may be explained by hybridization and a lack of informative characteristics.Our results do not support previous classification of the subgenera and sections within Cymbidium.Discordance between gene trees and network analysis indicate that reticulate evolution occurred in the genus Cymbidium.Overall,our study indicates that Cymbidium has undergone a complex evolution.
