Bacterial or viral infections,such as Brucella,mumps virus,herpes simplex virus,and Zika virus,destroy immune homeostasis of the testes,leading to spermatogenesis disorder and infertility.Of note,recent research shows...Bacterial or viral infections,such as Brucella,mumps virus,herpes simplex virus,and Zika virus,destroy immune homeostasis of the testes,leading to spermatogenesis disorder and infertility.Of note,recent research shows that SARS-CoV-2 can infect male gonads and destroy Sertoli and Leydig cells,leading to male reproductive dysfunction.Due to the many side effects associated with antibiotic therapy,finding alternative treatments for inflammatory injury remains critical.Here,we found that Dmrt1 plays an important role in regulating testicular immune homeostasis.Knockdown of Dmrt1 in male mice inhibited spermatogenesis with a broad inflammatory response in seminiferous tubules and led to the loss of spermatogenic epithelial cells.Chromatin immunoprecipitation sequencing(ChIP-seq)and RNA sequencing(RNA-seq)revealed that Dmrt1 positively regulated the expression of Spry1,an inhibitory protein of the receptor tyrosine kinase(RTK)signaling pathway.Furthermore,immunoprecipitation-mass spectrometry(IP-MS)and co-immunoprecipitation(Co-IP)analysis indicated that SPRY1 binds to nuclear factor kappa B1(NF-κB1)to prevent nuclear translocation of p65,inhibit activation of NF-κB signaling,prevent excessive inflammatory reaction in the testis,and protect the integrity of the blood-testis barrier.In view of this newly identified Dmrt1-Spry1-NF-κB axis mechanism in the regulation of testicular immune homeostasis,our study opens new avenues for the prevention and treatment of male reproductive diseases in humans and livestock.展开更多
An anisotropic micromechanics model based on the equivalent inclusion method is developed to investigate the rafting direction of Ni-based single crystal superalloys. The micromechanical model considers actual cubic s...An anisotropic micromechanics model based on the equivalent inclusion method is developed to investigate the rafting direction of Ni-based single crystal superalloys. The micromechanical model considers actual cubic structure and orthogonal anisotropy properties. The von Mises stress, elastic strain energy density, and hydrostatic pressure in dif- ferent inclusions of micromechanical model are calculated when applying a tensile or compressive loading along the [001] direction. The calculated results can successfully pre- dict the rafting direction for alloys exhibiting a positive or a negative mismatch, which are in agreement with pervious experimental and theoretical studies. Moreover, the elastic constant differences and mismatch degree of the matrix and precipitate phases and their influences on the rafting direction are carefully discussed.展开更多
The evolution of misfit dislocation network at γ/γ' phase interfaces and the stress distribution characteristics of Ni-based single-crystal superalloys under different temperatures of 0, 100 and 300 K are studied b...The evolution of misfit dislocation network at γ/γ' phase interfaces and the stress distribution characteristics of Ni-based single-crystal superalloys under different temperatures of 0, 100 and 300 K are studied by molecular dynamics (MD) simulation. It was found that a closed three-dimensional misfit dislocation network appears on the γ/γ' phase interfaces, and the shape of the dislocation network is independent of the lattice mismatch. Under the influence of the temperature, the dislocation network gradually becomes irregular, a/2 [110] dislocations in the γ matrix phase emit and partly cut into the γ' phase with the increase in temperature. The dislocation evolution is related to the local stress field, a peak stress occurs at γ/γ' phase interface, and with the increase in temperature and relaxation times, the stress in the γ phase gradually increases, the number of dislocations in the γ phase increases and cuts into γ' phase from the interfaces where dislocation network is damaged. The results provide important information for understanding the temperature dependence of the dislocation evolution and mechanical properties of Ni-based single-crystal superalloys.展开更多
In this paper,the morphology and evolution of interfacial dislocation networks of(100),(110)and(111)interphases of Ni-based single-crystal superalloys are studied by molecular dynamics(MD)simulations.Three-dimensional...In this paper,the morphology and evolution of interfacial dislocation networks of(100),(110)and(111)interphases of Ni-based single-crystal superalloys are studied by molecular dynamics(MD)simulations.Three-dimensional cubic-type and sandwich-type models are chosen to explore the orientation-dependent morphology of dislocation networks,and their respective advantages and disadvantages are compared.From the simulations,it is observed that various lattice orientations and model types lead to different morphologies of dislocation networks.Based on the analysis of average atomic energy and dislocation characteristics,the(100)orientation model has a more regular dislocation network,lower energy and better stability than the(110)and(111)orientation models after MD relaxation,which are supported by previous experimental and numerical simulations.Moreover,the cubic-type model has lower energy and better stability than the sandwich-type model.This will be helpful for choosing a more appropriate and reasonable model for simulating the interfacial dislocation networks of Ni-based single-crystal superalloys.展开更多
基金supported by the National Natural Science Foundation of China(32072806,31572399)National Key Research and Development Program of China(2022YFD1302201)+3 种基金Program of Shaanxi Province Science and Technology Innovation Team(2019TD-036)Major Projects of Natural Science Foundation of Inner Mongolia Autonomous Region(2020ZD10)Key Research and Development Program of Shaanxi Province(2022NY-044)Key Technologies Demonstration of Animal Husbandry in Shaanxi Province(20221086)。
文摘Bacterial or viral infections,such as Brucella,mumps virus,herpes simplex virus,and Zika virus,destroy immune homeostasis of the testes,leading to spermatogenesis disorder and infertility.Of note,recent research shows that SARS-CoV-2 can infect male gonads and destroy Sertoli and Leydig cells,leading to male reproductive dysfunction.Due to the many side effects associated with antibiotic therapy,finding alternative treatments for inflammatory injury remains critical.Here,we found that Dmrt1 plays an important role in regulating testicular immune homeostasis.Knockdown of Dmrt1 in male mice inhibited spermatogenesis with a broad inflammatory response in seminiferous tubules and led to the loss of spermatogenic epithelial cells.Chromatin immunoprecipitation sequencing(ChIP-seq)and RNA sequencing(RNA-seq)revealed that Dmrt1 positively regulated the expression of Spry1,an inhibitory protein of the receptor tyrosine kinase(RTK)signaling pathway.Furthermore,immunoprecipitation-mass spectrometry(IP-MS)and co-immunoprecipitation(Co-IP)analysis indicated that SPRY1 binds to nuclear factor kappa B1(NF-κB1)to prevent nuclear translocation of p65,inhibit activation of NF-κB signaling,prevent excessive inflammatory reaction in the testis,and protect the integrity of the blood-testis barrier.In view of this newly identified Dmrt1-Spry1-NF-κB axis mechanism in the regulation of testicular immune homeostasis,our study opens new avenues for the prevention and treatment of male reproductive diseases in humans and livestock.
基金supported by The National Natural Science Foundation of China (Grants 11102139 and 11472195)The Natural Science Foundation of Hubei Province of China (Grant 2014CFB713)
文摘An anisotropic micromechanics model based on the equivalent inclusion method is developed to investigate the rafting direction of Ni-based single crystal superalloys. The micromechanical model considers actual cubic structure and orthogonal anisotropy properties. The von Mises stress, elastic strain energy density, and hydrostatic pressure in dif- ferent inclusions of micromechanical model are calculated when applying a tensile or compressive loading along the [001] direction. The calculated results can successfully pre- dict the rafting direction for alloys exhibiting a positive or a negative mismatch, which are in agreement with pervious experimental and theoretical studies. Moreover, the elastic constant differences and mismatch degree of the matrix and precipitate phases and their influences on the rafting direction are carefully discussed.
基金financially supported by the National Natural Science Foundation of China(Nos.11102139 and 11472195)the Natural Science Foundation of Hubei Province of China(No.2014CFB713)
文摘The evolution of misfit dislocation network at γ/γ' phase interfaces and the stress distribution characteristics of Ni-based single-crystal superalloys under different temperatures of 0, 100 and 300 K are studied by molecular dynamics (MD) simulation. It was found that a closed three-dimensional misfit dislocation network appears on the γ/γ' phase interfaces, and the shape of the dislocation network is independent of the lattice mismatch. Under the influence of the temperature, the dislocation network gradually becomes irregular, a/2 [110] dislocations in the γ matrix phase emit and partly cut into the γ' phase with the increase in temperature. The dislocation evolution is related to the local stress field, a peak stress occurs at γ/γ' phase interface, and with the increase in temperature and relaxation times, the stress in the γ phase gradually increases, the number of dislocations in the γ phase increases and cuts into γ' phase from the interfaces where dislocation network is damaged. The results provide important information for understanding the temperature dependence of the dislocation evolution and mechanical properties of Ni-based single-crystal superalloys.
基金The work was supported by the National Natural Science Foundation of China(Grant Nos.11772236,11472195 and 11711530643).
文摘In this paper,the morphology and evolution of interfacial dislocation networks of(100),(110)and(111)interphases of Ni-based single-crystal superalloys are studied by molecular dynamics(MD)simulations.Three-dimensional cubic-type and sandwich-type models are chosen to explore the orientation-dependent morphology of dislocation networks,and their respective advantages and disadvantages are compared.From the simulations,it is observed that various lattice orientations and model types lead to different morphologies of dislocation networks.Based on the analysis of average atomic energy and dislocation characteristics,the(100)orientation model has a more regular dislocation network,lower energy and better stability than the(110)and(111)orientation models after MD relaxation,which are supported by previous experimental and numerical simulations.Moreover,the cubic-type model has lower energy and better stability than the sandwich-type model.This will be helpful for choosing a more appropriate and reasonable model for simulating the interfacial dislocation networks of Ni-based single-crystal superalloys.