BACKGROUND Abnormal tuftelin 1(TUFT1)has been reported in multiple cancers and exhibits oncogenic roles in tumor progression.However,limited data are available on the relationship between TUFT1 and hepatocellular carc...BACKGROUND Abnormal tuftelin 1(TUFT1)has been reported in multiple cancers and exhibits oncogenic roles in tumor progression.However,limited data are available on the relationship between TUFT1 and hepatocellular carcinoma(HCC),and the exact biological mechanism of TUFT1 is still poorly understood in HCC.AIM To investigate TUFT1 expression in HCC and how interfering TUFT1 transcription affects HCC growth.METHODS TUFT1 in HCC and non-HCC tissues based on databases of the Cancer Genome Atlas and Oncomine were analyzed,and TUFT1 in human HCC tissues on microarray were detected by immunohistochemistry for clinicopathological features,overall survival,and disease-free survival.HCC cells were transfected with constructed vectors of TUFT1 that interfere or over-express TUFT1 for analyzing the biological behaviors of HCC cells.Proliferation,invasion,migration,and apoptosis of cells were detected by cell counting kit-8,scratch assay,transwell tests,and flow cytometry and confirmed by Western blotting,respectively.RESULTS Abnormal TUFT1 levels in databases expressed in HCC at messenger RNA(mRNA)level and HCC tissues were mainly located in cytoplasm and membrane.The level of TUFT1 expression in the HCC group was significantly higher(χ2=18.563,P<0.001)than that in the non-cancerous group,closely related to clinical staging,size,vascular invasion of tumor,hepatitis B e-antigen positive,and ascites(P<0.01)of HCC patients,and negatively to HCC patients’overall survival and disease-free survival(P<0.001).After interfering with TUFT1 transcription at mRNA level in the MHCC-97H cells by the specific TUFT1-short hairpin RNA,cell proliferation,invasion,and metastasis were significantly inhibited with increasing apoptosis rate.In contrast,proliferation,invasion,and migration were significantly enhanced after over-expression of TUFT1 mRNA in Hep3B cells in vitro.CONCLUSION Oncogenic TUFT1 was associated with the progression of HCC and could be a potential molecular-target for inhibiting HCC growth.展开更多
BACKGROUND In the clinical treatment of diseases related to ureteral duplication,it is very important to make a clear diagnosis before surgery because different types of ureteral duplication correspond to different tr...BACKGROUND In the clinical treatment of diseases related to ureteral duplication,it is very important to make a clear diagnosis before surgery because different types of ureteral duplication correspond to different treatment options.Inverted Y ureteral duplication with ectopic ureters and multiple urinary calculi is clinically rare.This case can help clinicians increase their understanding of this disease and gain some experience in its diagnosis and treatment.CASE SUMMARY A 36-year-old male who was previously healthy presented to the hospital with lumbar pain.Percussion of the right kidney area showed the patient had pain.Computed tomography scans revealed multiple urinary calculi in the right urinary system.Computed tomography urography revealed a duplicated ureteral malformation with an ectopic ureter.A transurethral ureteroscopic holmium laser lithotripsy was performed successfully.Intraoperative retrograde ureterography was performed,and the ectopic ureter was visible.We informed the family of the intraoperative findings and suggested laparoscopic ectopic ureterectomy for the ectopic ureteral stones.Unfortunately,the family temporarily refused laparoscopic surgery.The patient did not feel any discomfort after one year of followup.CONCLUSION Inverted Y ureteral duplication with an ectopic ureter and multiple urinary calculi is rare.Clinicians must be highly vigilant,make a correct diagnosis before surgery,determine the type of ureteral duplication and the distribution of urinary calculi,and then draw up a reasonable treatment plan to avoid unnecessary complications.展开更多
基金Projects of the Ministry of Science and Technology,National Key Research and Development Program,No.2018YFC0116902National Natural Science Foundation of China,No.81673241,No.31872738,No.81873915.
文摘BACKGROUND Abnormal tuftelin 1(TUFT1)has been reported in multiple cancers and exhibits oncogenic roles in tumor progression.However,limited data are available on the relationship between TUFT1 and hepatocellular carcinoma(HCC),and the exact biological mechanism of TUFT1 is still poorly understood in HCC.AIM To investigate TUFT1 expression in HCC and how interfering TUFT1 transcription affects HCC growth.METHODS TUFT1 in HCC and non-HCC tissues based on databases of the Cancer Genome Atlas and Oncomine were analyzed,and TUFT1 in human HCC tissues on microarray were detected by immunohistochemistry for clinicopathological features,overall survival,and disease-free survival.HCC cells were transfected with constructed vectors of TUFT1 that interfere or over-express TUFT1 for analyzing the biological behaviors of HCC cells.Proliferation,invasion,migration,and apoptosis of cells were detected by cell counting kit-8,scratch assay,transwell tests,and flow cytometry and confirmed by Western blotting,respectively.RESULTS Abnormal TUFT1 levels in databases expressed in HCC at messenger RNA(mRNA)level and HCC tissues were mainly located in cytoplasm and membrane.The level of TUFT1 expression in the HCC group was significantly higher(χ2=18.563,P<0.001)than that in the non-cancerous group,closely related to clinical staging,size,vascular invasion of tumor,hepatitis B e-antigen positive,and ascites(P<0.01)of HCC patients,and negatively to HCC patients’overall survival and disease-free survival(P<0.001).After interfering with TUFT1 transcription at mRNA level in the MHCC-97H cells by the specific TUFT1-short hairpin RNA,cell proliferation,invasion,and metastasis were significantly inhibited with increasing apoptosis rate.In contrast,proliferation,invasion,and migration were significantly enhanced after over-expression of TUFT1 mRNA in Hep3B cells in vitro.CONCLUSION Oncogenic TUFT1 was associated with the progression of HCC and could be a potential molecular-target for inhibiting HCC growth.
基金Supported by Health Medicine Science and Technology Plan Project of Zhejiang Province,China,No.2021KY603.
文摘BACKGROUND In the clinical treatment of diseases related to ureteral duplication,it is very important to make a clear diagnosis before surgery because different types of ureteral duplication correspond to different treatment options.Inverted Y ureteral duplication with ectopic ureters and multiple urinary calculi is clinically rare.This case can help clinicians increase their understanding of this disease and gain some experience in its diagnosis and treatment.CASE SUMMARY A 36-year-old male who was previously healthy presented to the hospital with lumbar pain.Percussion of the right kidney area showed the patient had pain.Computed tomography scans revealed multiple urinary calculi in the right urinary system.Computed tomography urography revealed a duplicated ureteral malformation with an ectopic ureter.A transurethral ureteroscopic holmium laser lithotripsy was performed successfully.Intraoperative retrograde ureterography was performed,and the ectopic ureter was visible.We informed the family of the intraoperative findings and suggested laparoscopic ectopic ureterectomy for the ectopic ureteral stones.Unfortunately,the family temporarily refused laparoscopic surgery.The patient did not feel any discomfort after one year of followup.CONCLUSION Inverted Y ureteral duplication with an ectopic ureter and multiple urinary calculi is rare.Clinicians must be highly vigilant,make a correct diagnosis before surgery,determine the type of ureteral duplication and the distribution of urinary calculi,and then draw up a reasonable treatment plan to avoid unnecessary complications.
