GPRC5A调控的ABCB1表达对肺腺癌增殖的影响

目的ATP结合盒B亚家族成员1(ATP binding cassette subfamily B member 1,ABCB1)的异常表达在多种癌症的发生发展中发挥关键作用。然而,G蛋白偶联受体C家族5组A型(G protein coupled receptor family C group5 type A,GPRC5A)调控的ABCB1表达对肺腺癌增殖的影响仍不清楚。本研究探讨了GPRC5A调控的ABCB1表达对肺腺癌增殖的影响。方法我们采用RT-PCR、Western-blot或免疫组化实验,分析ABCB1在肺腺癌细胞系、人肺腺癌组织以及GPRC5A基因敲除小鼠和野生型小鼠的气管上皮细胞和肺组织中的表达。采用细胞计数试剂盒-8(CCK-8)分析GPRC5A基因敲除小鼠气管上皮细胞对化疗药物的敏感性。采用皮下肿瘤形成实验探讨下调ABCB1表达是否可抑制体内肺腺癌增殖。采用免疫荧光和免疫沉淀实验研究GPRC5A和ABCB1之间潜在的调控关系。结果ABCB1在肺腺癌细胞系和人类肺腺癌组织中表达上调。GPRC5A基因敲除小鼠的气管上皮细胞及肺组织的ABCB1表达高于野生型小鼠。与GPRC5A野生型小鼠的气管上皮细胞相比,GPRC5A基因敲除小鼠的气管上皮细胞对塔立奇达和多柔比星更敏感。注射移植细胞28天后,接受ABCB1基因敲除细胞移植的GPRC5A-/-C57BL/6小鼠的肺肿瘤的体积和重量均明显低于野生型细胞移植小鼠(P=0.0043,P=0.0060)。此外,免疫荧光和免疫沉淀实验表明,GPRC5A通过直接结合方式调控ABCB1的表达。结论GPRC5A通过抑制ABCB1表达降低肺腺癌增殖。GPRC5A调节ABCB1表达的途径有待研究。

Exploring the therapeutic effect of Xiaoyan d ecoction on lung cancer cachexia skeletal muscle atrophy based on L3-SMI

Background:Lung cancer cachexia has received widespread attention as one of the most common complications in patients with advanced lung cancer.As a multifactorial syndrome,lung cancer cachexia is characterized by a persistent decline in muscle mass that cannot be reversed by conventional nutrition Xiaoyan d ecoction can promote appetite and improve skeletal muscle mass in patients with lung cancer cachexia,while the third lumbar skeletal muscle index(L3-SMI)is able to determine whole-body skeletal muscle mass.To analyze the relationship between L3-SMI and hematological indexes and lung cancer cachexia,and to study the clinical efficacy of Xiaoyan decoction on skeletal muscle atrophy in lung cancer cachexia patients,with the aim of providing a reference basis for the early diagnosis and treatment of lung cancer cachexia patients and skeletal muscle atrophy.Methods:148 patients who were diagnosed with lung cancer in the Department of Oncology of the First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine from January 2020 to December 2022 were included,and were divided into cachexia and non-cachexia groups according to the diagnostic criteria of cachexia,and analyzed the differences of hematological indexes and L3-SMI between cachexia patients and non-cachexia patients.And the patients with cachexia were divided into control group and treatment group,analyzed and compared the changes of body mass index(BMI),L 3-SMI,Karnofsky functional status score,albumin and other hematological indexes of the two groups before and after the treatment,and evaluated the safety of the Xiaoyan decoction in the treatment of cachexia.Results:A total of 148 lung cancer patients were included in this study,including 67 patients in the cachexia group and 81 patients in the non-cachexia group.According to the pre-treatment statistical analysis,the BMI of patients in the cachexia group was lower than that of patients in the non-cachexia group(P<0.05);among the biochemical function indexes,the proportions of creatinine(P<0.05),total protein(P<0.05),The levels of albumin in the cachexia group were significantly lower(P<0.05)compared to the non-cachexia group;in the cachexia group,both males and females had lower L3-SMIs than in the non-cachexia group(P<0.05).A total of 62 cases of lung cancer cachexia were studied,30 cases in the control group and 32 cases in the treatment group,according to statistical analysis,BMI was significantly different before and after treatment(P<0.05);L3-SMI was significantly different in the treatment group before and after treatment(P<0.05);Karnofsky significantly differed in the treatment group before and after treatment(P<0.05);and there was a significant difference in albumin before and after(P<0.05).Conclusion:Cachexia patients had significantly lower third lumbar skeletal muscle mass than non-cachexia patients,according to this study;Xiaoyan decoction was able to improve skeletal muscle mass,nutritional status as well as functional status of patients with cachexia in lung cancer,among others.

An Ultra-wide Bandwidth Low-frequency Radio Astronomical Cryogenic Receiver for FAST Telescope

This paper presents an ultra-wide bandwidth(UWB)low-frequency radio astronomical cryogenic receiver for the Five-hundred-meter Aperture Spherical radio Telescope(FAST).It covers 6.6:1 bandwidth from 0.5 to 3.3 GHz.The receiver consists of a Quad-Ridged Flared Horn(QRFH),a cryogenic microwave unit,an optical transceiver and a warm microwave and frequency mixing unit.A QRFH with a concentric-loaded dielectric spear is developed:the average return losses are larger than 20 d B;the average ports polarization isolation is 43.87 d B;the average dish efficiency is higher than 65%.Many UWB cryogenic low loss components are developed for the fabrication of a cryogenic microwave unit.The average noise temperature lower than 14.2 K and 22.5 K are achieved as referred to the input ports of cryogenic Dewar and the output of horn,respectively.Compared to other similar advanced UWB receivers,such as Parkes 0.7-4.2 GHz(6:1 bandwidth)receiver and FAST 0.27-1.62 GHz(6:1 bandwidth)receiver,wider relative bandwidth of the proposed receiver is achieved and it is a new attempt to expand the bandwidth of UWB low-frequency receiver.

Analysis on Lump, Lumpoff and Rogue Waves with Predictability to a Generalized Konopelchenko-Dubrovsky-Kaup-Kupershmidt Equation

In this paper, we investigate a(2+1)-dimensional generalized Konopelchenko-Dubrovsky-Kaup-Kupershmidt equation. The lump waves, lumpoff waves, and rogue waves are presented based on the Hirota bilinear form of this equation. It is worth noting that the moving path as well as the appearance time and place of the lump waves are given. Moreover, the special rogue waves are considered when lump solution is swallowed by double solitons. Finally,the corresponding characteristics of the dynamical behavior are displayed.

Image interpretation: mining the visible and syntactic correlation of annotated words

Automatic web image annotation is a practical and effective way for both web image retrieval and image understanding. However, current annotation techniques make no further investigation of the statement-level syntactic correlation among the annotated words, therefore making it very difficult to render natural language interpretation for images such as "pandas eat bamboo". In this paper, we propose an approach to interpret image semantics through mining the visible and textual information hidden in images. This approach mainly consists of two parts: first the annotated words of target images are ranked according to two factors, namely the visual correlation and the pairwise co-occurrence; then the statement-level syntactic correlation among annotated words is explored and natural language interpretation for the target image is obtained. Experiments conducted on real-world web images show the effectiveness of the proposed approach.

The critical role of hot carrier cooling in optically excited structural transitions

The hot carrier cooling occurs in most photoexcitation-induced phase transitions(PIPTs),but its role has often been neglected in many theoretical simulations as well as in proposed mechanisms.Here,by including the previously ignored hot carrier cooling in real-time time-dependent density functional theory(rt-TDDFT)simulations,we investigated the role of hot carrier cooling in PIPTs.Taking IrTe2 as an example,we reveal that the cooling of hot electrons from the higher energy levels of spatially extended states to the lower energy levels of the localized Ir–Ir dimer antibonding states strengthens remarkably the atomic driving forces and enhances atomic kinetic energy.These two factors combine to dissolute the Ir–Ir dimers on a timescale near the limit of atomic motions,thus initiating a deterministic kinetic phase transition.We further demonstrate that the subsequent cooling induces nonradiative recombination of photoexcited electrons and holes,leading to the ultrafast recovery of the Ir–Ir dimers observed experimentally.These findings provide a complete picture of the atomic dynamics in optically excited structural phase transitions.