Early childhood caries (ECC) is a considerable pediatric and public health problem worldwide. Preceding studies have focused primarily on bacterial diversity at the taxonomic level. Although these studies have provi...Early childhood caries (ECC) is a considerable pediatric and public health problem worldwide. Preceding studies have focused primarily on bacterial diversity at the taxonomic level. Although these studies have provided significant information regarding the connection between dental caries and oral micmbiomes, further comprehension of this microbial community's ecological relevance is limited. This study identified the carbon source metabolic differences in dental plaque between children with and without ECC. We compared the microbial community functional diversity in 18 caries-free subjects with 18 severe ECC patients based on sole carbon source usage using a Biolog assay. The anaerobic microbial community in the ECC patients displayed greater metabolic activity than that of the control group. Specific carbon source metabolism differed significantly between the two groups. Subjects from the two groups were well distinguished by cluster and principal component analyses based on discriminative carbon sources. Our results implied that the microbial functional diversity between the ECC patients and healthy subjects differed significantly. In addition, the Biolog assay furthered our understanding of oral microbiomes as a composite of functional abilities, thus enabling us to identify the ecologically relevant functional differences among oral microbial communities.展开更多
Either potential flow or viscous flow based model may be flawed for numerical wave simulations.The two-way coupling of potential and viscous flow models with the domain decomposition utilizing respective strengths has...Either potential flow or viscous flow based model may be flawed for numerical wave simulations.The two-way coupling of potential and viscous flow models with the domain decomposition utilizing respective strengths has been a trending research topic.In contrast to existing literatures in which closed source potential models were used,the widely used open source OceanWave3D,OpenFOAM-v2012 are used in the present research.An innovative overlapping two-way coupling strategy is developed utilizing the ghost points in OceanWave3D.To guarantee computational stability,a relaxation zone used both for outlet damping and data transfer is built over the overlapping region in OceanWave3D.The free surface elevation in the relaxation zone is directly probed in OpenFOAM while the velocity potential is indirectly built upon its temporal variation which is calculated by the free surface boundary condition using the probed velocity.Strong coupling is achieved based on the fourth-order Runge-Kutta(RK)algorithm.Both two-and three-dimensional tests including linear,nonlinear,irregular,and multi-directional irregular waves,are conducted.The effectiveness of the coupling procedure in bidirectional data transfer is fully demonstrated,and the model is validated to be accurate and efficient,thus providing a competitive alternative for ocean wave simulations.展开更多
基金The National Natural Science Foundation of China(81200762 and 81470769)supported this work
文摘Early childhood caries (ECC) is a considerable pediatric and public health problem worldwide. Preceding studies have focused primarily on bacterial diversity at the taxonomic level. Although these studies have provided significant information regarding the connection between dental caries and oral micmbiomes, further comprehension of this microbial community's ecological relevance is limited. This study identified the carbon source metabolic differences in dental plaque between children with and without ECC. We compared the microbial community functional diversity in 18 caries-free subjects with 18 severe ECC patients based on sole carbon source usage using a Biolog assay. The anaerobic microbial community in the ECC patients displayed greater metabolic activity than that of the control group. Specific carbon source metabolism differed significantly between the two groups. Subjects from the two groups were well distinguished by cluster and principal component analyses based on discriminative carbon sources. Our results implied that the microbial functional diversity between the ECC patients and healthy subjects differed significantly. In addition, the Biolog assay furthered our understanding of oral microbiomes as a composite of functional abilities, thus enabling us to identify the ecologically relevant functional differences among oral microbial communities.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.52101324,52131102,51879159 and 52131102)the National Key Research and Development Program of China(Grant No.2019YFB1704200).
文摘Either potential flow or viscous flow based model may be flawed for numerical wave simulations.The two-way coupling of potential and viscous flow models with the domain decomposition utilizing respective strengths has been a trending research topic.In contrast to existing literatures in which closed source potential models were used,the widely used open source OceanWave3D,OpenFOAM-v2012 are used in the present research.An innovative overlapping two-way coupling strategy is developed utilizing the ghost points in OceanWave3D.To guarantee computational stability,a relaxation zone used both for outlet damping and data transfer is built over the overlapping region in OceanWave3D.The free surface elevation in the relaxation zone is directly probed in OpenFOAM while the velocity potential is indirectly built upon its temporal variation which is calculated by the free surface boundary condition using the probed velocity.Strong coupling is achieved based on the fourth-order Runge-Kutta(RK)algorithm.Both two-and three-dimensional tests including linear,nonlinear,irregular,and multi-directional irregular waves,are conducted.The effectiveness of the coupling procedure in bidirectional data transfer is fully demonstrated,and the model is validated to be accurate and efficient,thus providing a competitive alternative for ocean wave simulations.