Radiation therapy is a standard treatment for head and neck tumors.However,patients often exhibit cognitive impairments following radiation therapy.Previous studies have revealed that hippocampal dysfunction,specifica...Radiation therapy is a standard treatment for head and neck tumors.However,patients often exhibit cognitive impairments following radiation therapy.Previous studies have revealed that hippocampal dysfunction,specifically abnormal hippocampal neurogenesis or neuroinflammation,plays a key role in radiation-induced cognitive impairment.However,the long-term effects of radiation with respect to the electrophysiological adaptation of hippocampal neurons remain poorly characterized.We found that mice exhibited cognitive impairment 3 months after undergoing 10 minutes of cranial irradiation at a dose rate of 3 Gy/min.Furthermore,we observed a remarkable reduction in spike firing and excitatory synaptic input,as well as greatly enhanced inhibitory inputs,in hippocampal CA1 pyramidal neurons.Corresponding to the electrophysiological adaptation,we found reduced expression of synaptic plasticity marker VGLUT1 and increased expression of VGAT.Furthermore,in irradiated mice,long-term potentiation in the hippocampus was weakened and GluR1 expression was inhibited.These findings suggest that radiation can impair intrinsic excitability and synaptic plasticity in hippocampal CA1 pyramidal neurons.展开更多
Objective To explore the active components with toxic effects in five Aconitum L. herbal medicines on Tetrahymena thermophila. Methods The fingerprints of five Aconitum L. herbal medicines were established by ultra-hi...Objective To explore the active components with toxic effects in five Aconitum L. herbal medicines on Tetrahymena thermophila. Methods The fingerprints of five Aconitum L. herbal medicines were established by ultra-high performance liquid chromatography （UPLC） and the toxicity was evaluated by using a TAM Air Isothermal Calorimeter on Tetrahymena thermophila SB1 10. Results By analyzing the spectrum- effect relationships between UPLC fingerprints and toxic effects, the active components which had the toxic effects were obtained. Conclusion This work provides a general model of the combination of UPLC and microcalorimetry to study the spectrum-effect relationships of the five Aconitum L. herbal medicines, which could be used to evaluate the toxic effects and analyze the principal toxic components of the five Aconitum L. herbal medicines. On the whole, this result provides the experimental basis for the safe use of the five Aconitum L. herbal medicines in clinic.展开更多
Conventional organic solar cell＇s （OSC） architectures, including rigid transparent substrate （Glass）, conductive electrode （Indium tin oxide, ITO） and small working areas, are widely utilized in organic photovo...Conventional organic solar cell＇s （OSC） architectures, including rigid transparent substrate （Glass）, conductive electrode （Indium tin oxide, ITO） and small working areas, are widely utilized in organic photovoltaic fields. However, such a structure as well as conventional spin-coating method obviously restrict their industrial application. In this article, we report the deposition of silver nanowires （AgNWs） on the flexible substrate by slot-die printing. The obtained AgNWs films exhibited a high transmittance and a low resistance, and were further used as the transparent conductive electrode ofOSCs. A typical conjugated polymer, poly[（2-5-bis（2-hexyldecyloxy）phenylene）-alt-（5,6-difluoro-4,7-di（thiophen-2-yl）benzo[c] [1,2,5]thiadiazole）] （PPDT2FBT）, was used as the active material to fabricate large-area （7 cm2） solar cells by a slot-die coating process. The power conversion efficiency （PCE） could reach 1.87% initially and further increased to 3.04% by thermal annealing. Compared to the performance of reference cell on ITO substrate, the result indicated that the AgNWs could be developed as an alternative substitute of conductive electrode to fabricate the large-area flexible OSCs by roll-to-roll printing.展开更多
基金supported by the National Natural Science Foundation of China,Nos.81925031(to YT),81820108026(to YT),81972967(to WJL),81872549(to YL)the Youth Program of National Natural Science Foundation of China,No.81801229(to YTX)+3 种基金a grant from Guangdong Science and Technology Department of China,Nos.2020B1212060018(to WJL),2020B1212030004(to WJL)the Natural Science Foundation of Guangdong Province,No.2019A1515011754(to WJL)the Science and Technology Program of Guangzhou of China,No.202007030001(to YT)the Science and Technology Planning Project of Guangzhou of China,No.201704030033(to YL).
文摘Radiation therapy is a standard treatment for head and neck tumors.However,patients often exhibit cognitive impairments following radiation therapy.Previous studies have revealed that hippocampal dysfunction,specifically abnormal hippocampal neurogenesis or neuroinflammation,plays a key role in radiation-induced cognitive impairment.However,the long-term effects of radiation with respect to the electrophysiological adaptation of hippocampal neurons remain poorly characterized.We found that mice exhibited cognitive impairment 3 months after undergoing 10 minutes of cranial irradiation at a dose rate of 3 Gy/min.Furthermore,we observed a remarkable reduction in spike firing and excitatory synaptic input,as well as greatly enhanced inhibitory inputs,in hippocampal CA1 pyramidal neurons.Corresponding to the electrophysiological adaptation,we found reduced expression of synaptic plasticity marker VGLUT1 and increased expression of VGAT.Furthermore,in irradiated mice,long-term potentiation in the hippocampus was weakened and GluR1 expression was inhibited.These findings suggest that radiation can impair intrinsic excitability and synaptic plasticity in hippocampal CA1 pyramidal neurons.
基金State Natural Science Foundation(81173571)The"Twelfth Five-Year Plan"Foundation of China People’s Liberation Army(CWS11C164)The Major Projects of the National Science and Technology(2012ZX10005010-002-002)
文摘Objective To explore the active components with toxic effects in five Aconitum L. herbal medicines on Tetrahymena thermophila. Methods The fingerprints of five Aconitum L. herbal medicines were established by ultra-high performance liquid chromatography （UPLC） and the toxicity was evaluated by using a TAM Air Isothermal Calorimeter on Tetrahymena thermophila SB1 10. Results By analyzing the spectrum- effect relationships between UPLC fingerprints and toxic effects, the active components which had the toxic effects were obtained. Conclusion This work provides a general model of the combination of UPLC and microcalorimetry to study the spectrum-effect relationships of the five Aconitum L. herbal medicines, which could be used to evaluate the toxic effects and analyze the principal toxic components of the five Aconitum L. herbal medicines. On the whole, this result provides the experimental basis for the safe use of the five Aconitum L. herbal medicines in clinic.
基金financially supported by the National Natural Science Foundation of China(Nos.21125420 and 21474022)the Chinese Academy of Sciences
文摘Conventional organic solar cell＇s （OSC） architectures, including rigid transparent substrate （Glass）, conductive electrode （Indium tin oxide, ITO） and small working areas, are widely utilized in organic photovoltaic fields. However, such a structure as well as conventional spin-coating method obviously restrict their industrial application. In this article, we report the deposition of silver nanowires （AgNWs） on the flexible substrate by slot-die printing. The obtained AgNWs films exhibited a high transmittance and a low resistance, and were further used as the transparent conductive electrode ofOSCs. A typical conjugated polymer, poly[（2-5-bis（2-hexyldecyloxy）phenylene）-alt-（5,6-difluoro-4,7-di（thiophen-2-yl）benzo[c] [1,2,5]thiadiazole）] （PPDT2FBT）, was used as the active material to fabricate large-area （7 cm2） solar cells by a slot-die coating process. The power conversion efficiency （PCE） could reach 1.87% initially and further increased to 3.04% by thermal annealing. Compared to the performance of reference cell on ITO substrate, the result indicated that the AgNWs could be developed as an alternative substitute of conductive electrode to fabricate the large-area flexible OSCs by roll-to-roll printing.
