Objective To explore the relationships intergenic region in mtDNA with PCOS. of variations in D-loop and COH-tRNA^Lys Methods A total of 77 PCOS and 45 non-PCOS patients were enrolled, whose D-loop and COH-tRNA^Lys in...Objective To explore the relationships intergenic region in mtDNA with PCOS. of variations in D-loop and COH-tRNA^Lys Methods A total of 77 PCOS and 45 non-PCOS patients were enrolled, whose D-loop and COH-tRNA^Lys intergenic region in mtDNA were amplified and sequenced; sexual hormone assay, oral glucose tolerance test （OGTT） and insulin releasing test were carried out. Then variations found in mtDNA were compared between the two groups, the correlations between variations and clinical indexes were analyzed in all subjects. Results Nucleotide variations found in mtDNA were not different between the two groups, but the mutation of 16 094T/C was found associated with the serum levels ofT and fasting insulin; （303-317）CnTC, associated with the serum levels of A and LH; 195C/T with A level and 491T/C with LH level; （8 272-8 289）（ACCCCCTCT）, was associated with the serum level of 1 h glucose. Conclusion Noncoding region mutations in mtDNA perhaps associate with PCOS clinical symptoms and involve in PCOS development.展开更多
基金supported by a grant from the Foundation of Ministry of Education of China (No. JHG06-7-03)
文摘Objective To explore the relationships intergenic region in mtDNA with PCOS. of variations in D-loop and COH-tRNA^Lys Methods A total of 77 PCOS and 45 non-PCOS patients were enrolled, whose D-loop and COH-tRNA^Lys intergenic region in mtDNA were amplified and sequenced; sexual hormone assay, oral glucose tolerance test （OGTT） and insulin releasing test were carried out. Then variations found in mtDNA were compared between the two groups, the correlations between variations and clinical indexes were analyzed in all subjects. Results Nucleotide variations found in mtDNA were not different between the two groups, but the mutation of 16 094T/C was found associated with the serum levels ofT and fasting insulin; （303-317）CnTC, associated with the serum levels of A and LH; 195C/T with A level and 491T/C with LH level; （8 272-8 289）（ACCCCCTCT）, was associated with the serum level of 1 h glucose. Conclusion Noncoding region mutations in mtDNA perhaps associate with PCOS clinical symptoms and involve in PCOS development.
