Microglial NLRP3 inflammasome-mediated neuroinflammation and therapeutic strategies in depression

Previous studies have demonstrated a bidirectional relationship between inflammation and depression.Activation of the nucleotide-binding oligomerization domain,leucine-rich repeat,and NLR family pyrin domain-containing 3(NLRP3)inflammasomes is closely related to the pathogenesis of various neurological diseases.In patients with major depressive disorder,NLRP3 inflammasome levels are significantly elevated.Understanding the role that NLRP3 inflammasome-mediated neuroinflammation plays in the pathogenesis of depression may be beneficial for future therapeutic strategies.In this review,we aimed to elucidate the mechanisms that lead to the activation of the NLRP3 inflammasome in depression as well as to provide insight into therapeutic strategies that target the NLRP3 inflammasome.Moreover,we outlined various therapeutic strategies that target the NLRP3 inflammasome,including NLRP3 inflammatory pathway inhibitors,natural compounds,and other therapeutic compounds that have been shown to be effective in treating depression.Additionally,we summarized the application of NLRP3 inflammasome inhibitors in clinical trials related to depression.Currently,there is a scarcity of clinical trials dedicated to investigating the applications of NLRP3 inflammasome inhibitors in depression treatment.The modulation of NLRP3 inflammasomes in microglia holds promise for the management of depression.Further investigations are necessary to ascertain the efficacy and safety of these therapeutic approaches as potential novel antidepressant treatments.

A geographical similarity-based sampling method of non-fire point data for spatial prediction of forest fires

Understanding the mechanisms and risks of forest fires by building a spatial prediction model is an important means of controlling forest fires.Non-fire point data are important training data for constructing a model,and their quality significantly impacts the prediction performance of the model.However,non-fire point data obtained using existing sampling methods generally suffer from low representativeness.Therefore,this study proposes a non-fire point data sampling method based on geographical similarity to improve the quality of non-fire point samples.The method is based on the idea that the less similar the geographical environment between a sample point and an already occurred fire point,the greater the confidence in being a non-fire point sample.Yunnan Province,China,with a high frequency of forest fires,was used as the study area.We compared the prediction performance of traditional sampling methods and the proposed method using three commonly used forest fire risk prediction models:logistic regression(LR),support vector machine(SVM),and random forest(RF).The results show that the modeling and prediction accuracies of the forest fire prediction models established based on the proposed sampling method are significantly improved compared with those of the traditional sampling method.Specifically,in 2010,the modeling and prediction accuracies improved by 19.1%and 32.8%,respectively,and in 2020,they improved by 13.1%and 24.3%,respectively.Therefore,we believe that collecting non-fire point samples based on the principle of geographical similarity is an effective way to improve the quality of forest fire samples,and thus enhance the prediction of forest fire risk.

OsNRAMP2 facilitates Cd efflux from vacuoles and contributes to the difference in grain Cd accumulation between japonica and indica rice

Cadmium(Cd)accumulation in rice grain is of health concern.Identifying genes involved in grain Cd accumulation and performing molecular breeding may reduce it.In this study,knockout of OsNRAMP2,a member of the NRAMP family,reduced grain Cd concentrations by more than 38%,and overexpressing OsNRAMP2 increased grain Cd concentrations by more than 50%.Physiological experiments showed that OsNRAMP2 facilitated Cd translocation from root to shoot by positively regulating Cd efflux from the vacuoles.At filling stage,OsNRAMP2 was highly expressed in all tissues except for husk,suggesting its role in Cd remobilization.Changes in OsNRAMP2 expression affected the concentrations of Fe,Mn,Zn,and Cu in grain and also affected rice growth.Phylogenetic analysis showed that the distribution of OsNRAMP2 haplotypes between japonica and indica was different.Among the four haplotypes of OsNRAMP2,Hap 1,with a 6-bp nucleotide insertion in exon 1,had grain Cd concentration at least 45.3%lower than any of the other three haplotypes.Almost all(99.3%)japonica accessions but rare indica accessions(4.44%)from the 3K sequenced rice genomes carry Hap 1 of OsNRAMP2.Our study sheds light on the molecular mechanism of grain Cd accumulation and provides a promising target for low-Cd rice breeding.

Three-Dimensional Analytical Modeling of Axial-Flux Permanent Magnet Drivers

In this paper, the axial-flux permanent magnet driver is modeledand analyzed in a simple and novel way under three-dimensional cylindricalcoordinates. The inherent three-dimensional characteristics of the deviceare comprehensively considered, and the governing equations are solved bysimplifying the boundary conditions. The axial magnetization of the sectorshapedpermanent magnets is accurately described in an algebraic form bythe parameters, which makes the physical meaning more explicit than thepurely mathematical expression in general series forms. The parameters of theBessel function are determined simply and the magnetic field distribution ofpermanent magnets and the air-gap is solved. Furthermore, the field solutionsare completely analytical, which provides convenience and satisfactoryaccuracy for modeling a series of electromagnetic performance parameters,such as the axial electromagnetic force density, axial electromagnetic force,and electromagnetic torque. The correctness and accuracy of the analyticalmodels are fully verified by three-dimensional finite element simulations and a15 kW prototype and the results of calculations, simulations, and experimentsunder three methods are highly consistent. The influence of several designparameters on magnetic field distribution and performance is studied and discussed.The results indicate that the modeling method proposed in this papercan calculate the magnetic field distribution and performance accurately andrapidly, which affords an important reference for the design and optimizationof axial-flux permanent magnet drivers.

Relationship between the Ecological Livability and the Green Development of Agriculture

Ecological livability is one of the important tasks and objectives of the rural revitalization strategy proposed at the 19 th National Congress of the CPC.The most precious wealth of the countryside is the excellent ecological environment,and China must unswervingly follow the route of green agricultural development.Based on current research on the relationship between ecological livable and agricultural green development,comprehensive policy documents,journal literature and related reports,this paper explored the background and connotation of ecological livable,the current situation of agricultural green development and existing problems,and profoundly analyzed ecological livable and the internal connection between agricultural green development,aiming to provide theoretical reference and scientific reference for the final realization of ecological livable goal.

Regeneration Characteristics and Spatial Pattern of <i>Platycladus orientalis</i>in Mount Tai, China

Platycladus orientalis is one of the main tree species in Mount Tai, and its sustainable development is of great significance to the protection and development of the forest ecological environment of Mount Tai. In this study, a representative sample plot of Platycladus orientalis with a size of 30 m * 20 m was selected on Mount Tai. The growth and distribution of the parent trees and seedlings of Platycladus orientalis were investigated in the sample plots. The results showed that both the mother cypress trees and seedlings inside the plot present an aggregated distribution pattern within a certain scale, which conforms to the distribution of most natural communities;and the mother cypress can promote seedlings within a certain scale. Although Platycladus orientalis has good natural regeneration ability, it is difficult to succeed in understory regeneration solely relying on natural fertility, and it is not conducive to the long-term stable development of forest stands. Therefore, the thinning of Platycladus orientalis and the adjustment of the forest stand structure and artificial promotion of natural regeneration can not only reduce operating costs, but also conform to the idea of forest near-natural management and maintain sustainable forest development.

Cyanidin-3-O-glucoside alleviates trimethyltin chloride-induced neurodegeneration by maintaining glutamate homeostasis through modulation of the gut microbiota

Trimethyltin chloride(TMT)is a potent neurotoxin to cause neurodegeneration,especially in hippocampus.This study aimed to identify dietary components that can effectively attenuate TMT-induced neurodegeneration in humans.The predominant anthocyanin in human diets,cyanidin-3-O-glucoside(C3G,5 or 50 mg/kg),was given to mice for 16 days,and TMT(2.7 mg/kg)was injected intraperitoneally once on the eighth day.C3G(50 mg/kg)significantly alleviated TMT-induced seizures and subsequent cognitive impairment by ameliorating hippocampal neurodegeneration and synaptic dysfunction.Furthermore,C3G treatment restored glutamate homeostasis in brain and reversed glutamine synthetase(GS)inhibition in reactive astrogliosis and neuroinflammation,which are critical for C3G's neuroprotective effects.Notably,C3G decreased the lipopolysaccharide,tumor necrosis factor-α,interleukin-6,and interleukin-1βlevels in the mice,which potentially by modulating the relative abundance of Atopobiaceae and Lachnospiraceae in the gut.C3G may be a promising and practical dietary component for reducing TMT-induced neurodegeneration.

Food-derived protein hydrolysates and peptides:anxiolytic and antidepressant activities,characteristics,and mechanisms

Globally,the prevalence of anxiety and depression has reached epidemic proportions.Food-derived protein hydrolysates and peptides delivered through dietary supplementation can avoid the negative risks associated with traditional pharmaceuticals while delivering superior anxiolytic and antidepressant effects.This review summarizes current research on food-derived anxiolytic and antidepressant protein hydrolysates and peptides,and subsequently analyses their physicochemical characteristics and elaborates on their mechanisms.The aim of this work is to contribute to the in-depth study and provide a theoretical foundation for the development of related products to better serve patients with anxiety and depression.

High-resolution crustal structure of the Yinchuan basin revealed by deep seismic reflection profiling:implications for deep processes of basin

The Yinchuan basin, located on the western margin of the Ordos block, has the characteristics of an active continental rift. A NW-striking deep seismic reflection profile across the center of Yinchuan basin precisely revealed the fine structure of the crust. The images showed that the crust in the Yinchuan basin was characterized by vertical stratifications along a detachment located at a two-way travel time(TWT) of 8.0 s.The most outstanding feature of this seismic profile was the almost flat Mohorovicˇic′ discontinuity(Moho) and a high-reflection zone in the lower crust. This sub-horizontal Moho conflicts with the general assumption of an uplifted Moho under sedimentary basins and continental rifts, and may indicate the action of different processes at depth during the evolution of sedimentary basins or rifts.We present a possible interpretation of these deep processes and the sub-horizontal Moho. The high-reflection zone, which consists of sheets of high-density, mantlederived materials, may have compensated for crustal thinning in the Yinchuan basin, leading to the formation of a sub-horizontal Moho. These high-density materials may have been emplaced by underplating with mantlesourced magma.

Using large dynamite shots to image the structure of the Moho from deep seismic reflection experiment between the Sichuan basin and Qinling orogen

The Qinling orogen was formed as a result of the collision between the North and South China blocks. The Qinling orogen represents the location at which the southern and northern parts of the Chinese mainland collided, and it's also the intersection of the Central China orogen and the north-south tectonic belt. There is evidence of strong deformation in this orogen, and it has had a long and complex geological history. We investigated the structure of the Moho in the southern Qinling orogen using large dynamite shot imaging techniques. By integrating the analysis of the single-shot and the move-out corrections profile, we determined the structure of the Moho beneath the northern Dabashan thrust belt and the southern Qinling orogen, including the mantle suture beneath Fenghuang mountain. The Moho is divided into two parts by the mantle suture zone beneath Fenghuang mountain:(1) from Ziyang to Hanyin, the north-dipping Moho is at about45–55 km depth and the depth increases rapidly; and(2)from Hanyin to Ningshan, the south-dipping Moho is at about 40–45 km depth and shallows slowly. The mantle suture is located beneath Fenghuang mountain, and the Moho overlaps at this location: the shallower Moho is connected to the northern part of China, and the deeper Moho is connected to the southern part. This may indicate that the lithosphere in the Sichuan basin subducts to the Qinling block and that the subduction frontier reaches at least as far as Fenghuang mountain.

Application of active-source surface waves in urban underground space detection: A case study of Rongcheng County, Hebei, China

Active-source surface wave exploration is advantageous because it has high imaging accuracy,is not affected by high-speed layers,and has a low cost;thus,it has unique advantages for investigating shallow surface structures.For the development and utilization of urban underground space,two parameters in the shallow surface are important,namely,the shear wave velocity(V_(S))and the predominant period of the site,which determine the elevation and aseismic grade of the building design.The traditional method is mainly to obtain the two above-mentioned parameters through testing and measuring drilling samples.However,this method is extremely expensive and time consuming.Therefore,in this research,we used the multichannel surface wave acquisition method to extract the fundamental dispersion curve of single-shot data by using the phase shift method and obtain the V_(S) characteristics in the uppermost 40 m by inversion.We arrived at the following two conclusions based on the V_(S) profile.First,the study area can be roughly divided into five layers,among which the layers 0−8 m,14−20 m,and 20−30 m are low-velocity layers,corresponding to miscellaneous fill,a water-bearing sand layer,and a sand layer;therefore,the V_(S) is relatively low.In contrast,the layers at 8−14 m and 30−40 m are high-velocity layers that are mainly composed of clay,with a relatively better compactness and relatively high V_(S) values.In addition,a low-speed anomaly appears abruptly in the high-speed area at 20−40 m.This anomaly,when combined with geological data,suggests that it is an ancient river channel.Second,from the V_(S) value,the V_(Se)(equivalent shear wave velocity)was calculated.The construction site soil was categorized as class III,with good conditions for engineering geology.In addition,we calculated the predominant period of the site to be 0.56-0.77 s based on the V_(S).Therefore,in the overall structural design of the foundation engineering,the natural vibration period of the structure should be strictly controlled to avoid the predominant period of the site.

The Clinical Anatomy of the Cavernous Sinus

Introduction: The cavernous sinus (CS) is a very important concept because it is not only interesting to anatomical theory but also useful to clinical medicine, especially in the field of surgery. This paper described the microsurgical anatomy of the CS with special attention to its concept that the CS was really venous sinus or plexus. Materials and Methods: Fifty CSs from 25 Chinese adult cadaver heads fixed in 10% methanal, whose artery and vein were injected with red and blue latex, respectively, dissected stepwise under the operating microscope. Results: Asymmetric and nonintegral blue latex distributed in the cavity of the CS to form a retina with various diameters and repeatedly diverged and converged were observed under the surgical microscope with magnification 5 - 25, after the lateral wall of the CS was opened by maxillary approach. Measurement of sinus included length, diameter and triangular structure of the CS. It is very important to understand the microsurgical anatomy of the CS for neurosurgeons. Conclusion: The CS was venous plexus rather than sinus. The lateral wall of the sinus had two layers, and the lateral cavity of the sinus really did exist even though it was very small. The triangles where maxillary approach passed were more important for neurosurgeons.

Electrochemical Biosensor for Detection of Staphylococcus aureus Based on Nucleic Acid Aptamers

Staphylococcus aureus is a gram-staining positive cocci bacillus baterium and also one of the foodborne pathogens, which is a serious potential hazard to human health and food safety. We constructed an electrochemical biosensor for the detection of S. aureus based on nucleic acid aptamers to achieve highly specific detection of S. aureus. The detection of S. aureus was realized by using Aptamer (Apt) to capture S. aureus, which resulted in a change in the spatial conformation of Apt and a decrease in the electrochemical signal. Under the optimized experimental conditions, the detected electrochemical signals were positively correlated with the concentration of S. aureus with a linear range of 1 × 101 - 1 × 105 CFU/mL, a detection limit of 4.76 CFU/mL, and an experimental recovery of 97.43% - 99.37%. Therefore, we successfully constructed an electrochemical biosensor for the specific detection of S. aureus, which has the advantages of high specificity, sensitive detection and convenient operation.

Exploration of Nature Education Path for Teenagers:A Case Study of Yancheng Wetland Nature Education Based on the World Natural Heritage Site

Nature education is the trend of the current social development.By gradually improving the awareness of ecological civilization among the whole people,the achievements in ecological civilization are jointly built and shared by the whole people.Yancheng Yellow Sea Wetland is a characteristic natural resource and the name card of Yancheng City,and also an important part of the natural education of teenagers in Yancheng.As one of the main resources of nature education in Yancheng,the Yellow Sea Wetland World Natural Heritage resources play a more important role in the cultivation of youth ecological civilization values and legal awareness of environmental protection.Based on the protection and utilization of the Yellow Sea Wetland World Natural Heritage resources,this paper explores the methods and approaches of nature education for teenagers in Yancheng.

Two material removal modes in chemical mechanical polishing:mechanical plowing vs.chemical bonding

With the rapid development of semiconductors,the number of materials needed to be polished sharply increases.The material properties vary significantly,posing challenges to chemical mechanical polishing(CMP).Accordingly,the study aimed to classify the material removal mechanism.Based on the CMP and atomic force microscopy results,the six representative metals can be preliminarily classified into two groups,presumably due to different material removal modes.From the tribology perspective,the first group of Cu,Co,and Ni may mainly rely on the mechanical plowing effect.After adding H_(2)O_(2),corrosion can be first enhanced and then suppressed,affecting the surface mechanical strength.Consequently,the material removal rate(MRR)and the surface roughness increase and decrease.By comparison,the second group of Ta,Ru,and Ti may primarily depend on the chemical bonding effect.Adding H_(2)O_(2)can promote oxidation,increasing interfacial chemical bonds.Therefore,the MRR increases,and the surface roughness decreases and levels off.In addition,CMP can be regulated by tuning the synergistic effect of oxidation,complexation,and dissolution for mechanical plowing,while tuning the synergistic effect of oxidation and ionic strength for chemical bonding.The findings provide mechanistic insight into the material removal mechanism in CMP.

New taxa of Berberis (Berberidaceae) with greenish flowers from a biodiversity hotspot in Sichuan Province, China

A new species,Berberis viridiflora X.H.Li,and a new variety,Berberis sanguinea Franch.var.viridisepala X.H.Li,L.C.Zhang & W.H.Li are described and illustrated from Baoxing County,a biodiversity hotspot located on the eastern edge of Hengduan Mountains in Sichuan Province,Southwest China,Both new taxa resemble B.sanguinea Franch.var.sanguinea,but B.viridiflora differs by the greenish flowers,and the petals being truncate,obtuse,or undulate at apex;while B.sanguinea var.viridisepala differs by the greenish or yellowish green flowers.Morphological features of the pollen grains of B.sanguinea and the two new taxa are revealed by scanning election microscope.B.sanguinea var.sanguinea displays obvious similarities with a sympatric congener,Berberis multiovula T.S.Ying in the morphology of flowers,stems and leaves,especially its ovule number varies greatly from 2 to 9,thus,B.multiovula characterized by the5-ovuled ovary is reduced to a synonymy of B.sanguinea var.sanguinea.On the basis of field surveys and study of herbarium specimens,a total of 16 species and varieties of Berberis are recorded from Baoxing County,and a key is provided.Among the 16 taxa,most phenotypic variations in Chinese Berberis can be displayed,including the habit of plants,most morphological variations of stems,branches and leaves,all types of inflorescences,all color types of flowers,and nearly all types of the shape and color of fruits.Baoxing County and its adjacent Sichuan Giant Panda Sanctuaries possibly constitute an active diversification center of Berberis in eastern Hengduan Mountains of China.

Variation of Moho Depth across Bangong-Nujiang Suture in Central Tibet—Results from Deep Seismic Reflection Data

There is a long-term dispute at Moho depth across the Bangong-Nujiang suture (BNS). Due to the complicated and changeable seismic geological condition, it is not easy to acquire images of the reflective Moho in central Tibet. In the support of the SinoProbe project, a series of deep seismic reflection profiles were conducted to image Moho structure across the BNS and the Qiangtang terrane. These profiles extend from the northern Lhasa terrane to the Qiangtang terrane crossing the BNS. Both shot gathers and migration data show clear Moho images beneath the BNS. The Moho depth varies from 75.1 km (~24 s TWT) beneath the northmost Lhasa terrane to 68.9 km (~22 s TWT) beneath southmost Qiangtang terrane, and rises smoothly to 62.6 km (~20 s TWT ) at ~28 km north of the BNS beneath the Qiangtang terrane. We speculate that the Moho appears a 6.2 km sharp offset across the BNS and becomes ~12.5 km shallower from the northmost Lhasa terrane to the south Qiangtang terrane at ~28 km north of the BNS. The viewpoint of Moho depth across the BNS based on deep seismic reflection data is inconsistent with the previous 20 km offset.

基于体细胞突变数据库筛选免疫原性结直肠癌高频新抗原的方法

结直肠癌是世界高发和高致死率的恶性肿瘤。靶向新抗原的免疫治疗已被证实可以诱导癌症患者肿瘤持续消退,但这些特异性新抗原,仅适用于个体精准治疗。随着大量的高频肿瘤基因突变被发现,这些与突变相关的高频新抗原可覆盖更多人群,具有较强的临床意义。然而目前结直肠癌中是否也存在高频新抗原仍不清楚。本研究利用来源于321个结直肠癌患者的体细胞突变数据库,联合1种标准过滤和7种预测算法,筛选并获得了25个基于中国人高频分型HLA-A^*1101限制性的高频新抗原,它们均具有高亲和力(IC50<50 nmol/L)和高呈递分值(>0.90);其中,除了阳性对照多肽KRAS_G12V8-16外,11个高频新抗原能够在体外诱导细胞毒性T淋巴细胞(cytotoxic T lymphocyte,CTL)分泌γ干扰素(interferon gamma,IFN-γ),证实具有免疫原性。选取免疫原性最强的新抗原C1orf170_S418G413-421及阳性对照多肽KRAS_G12V8-16体外刺激T细胞,利用流式细胞分选及单细胞转录组测序技术,获得其特异性CTL的免疫组库信息,所构建的TCR-T(T-cell receptor engineered T cell)能够识别新抗原并分泌细胞因子。以上结果表明,本研究开发了一种利用体细胞数据库预测并体外筛选验证具有免疫原性高频新抗原的方法,为结直肠癌及其他癌种的多肽、DC(dendritic cells)疫苗、TCR-like抗体、TCR-T等免疫治疗提供了重要的多肽靶点和TCR信息,具有实际的临床应用价值。

^(89)锶联合云克治疗晚期乳腺癌骨转移80例疗效观察(英文)

Objective: The aim of our study was to evaluate the efficacy of 89 Sr combined with Technetium [99Tc] Methylenediphosphonate Injection(99Tc-MDP) in the treatment of cancer pain in the advanced breast cancers with bone metastases. Methods: A total of 80 patients with various degrees of bone pain due to multiple metastases of breast cancer were treated with 89 Sr combined with 99Tc-MDP. 89 Sr was given intravenously at 4m Ci on day 1 during the 3-month schedule. After 7 days, 99Tc-MDP was given at 22 mg/day on days 1–10 during the 1-month schedule, for 3 to 6 months. Results: The effective rate of relieving pain was 83.75%. The effective rate of curing bone metastases was 81.25%. So there was a significant improvement in the quality of life of the patients. Conclusion: 89 Sr combined with 99Tc-MDP are effective in the treatment of cancer pain in the breast cancers with bone metastasis, and can obviously repair the bone destruction caused by metastases, thereby improving the quality of life in advanced breast cancer patients with bone metastases.

任务类型对混合学习投入度的影响研究

混合学习投入度是有效衡量混合学习质量的重要指标。目前关于学习投入度影响因素的研究主要围绕个体、人际关系、学习环境等方面展开,容易忽视学习任务,即不同任务类型对学生混合学习投入度的影响。文章以中国大学MOOC平台导出的慕课学习成绩和混合学习投入量表为测量工具,采用问卷调查法对337名参与混合学习的学前教育专业学生进行调查,结果发现大学生混合学习投入度整体水平处于中等偏上,且线上学习中的慕课总成绩与混合学习投入度之间呈显著正相关;混合学习投入度中行为、认知、情感投入的得分均处于中等偏上水平,且情感投入的标准差相对较小;不同任务类型在学生混合学习投入度上存在显著差异,事后检验发现,课堂学习任务类型的混合学习投入度水平高于在线学习任务类型的混合学习投入度水平。