Trust Score-Based Malicious Vehicle Detection Scheme in Vehicular Network Environments

Advancements in the vehicular network technology enable real-time interconnection,data sharing,and intelligent cooperative driving among vehicles.However,malicious vehicles providing illegal and incorrect information can compromise the interests of vehicle users.Trust mechanisms serve as an effective solution to this issue.In recent years,many researchers have incorporated blockchain technology to manage and incentivize vehicle nodes,incurring significant overhead and storage requirements due to the frequent ingress and egress of vehicles within the area.In this paper,we propose a distributed vehicular network scheme based on trust scores.Specifically,the designed architecture partitions multiple vehicle regions into clusters.Then,cloud supervision systems(CSSs)verify the accuracy of the information transmitted by vehicles.Additionally,the trust scores for vehicles are calculated to reward or penalize them based on the trust evaluation model.Our proposed scheme demonstrates good scalability and effectively addresses the main cause of malicious information distribution among vehicles.Both theoretical and experimental analysis show that our scheme outperforms the compared schemes.

Progress in the Study of False Smut Disease in Rice

Rice false smut disease is an increasing concern for research and production, not only because of the increasing epidemic occurrence in rice production, but also the intriguing specific pathogenesis of the disease to be a unique pathological system to enrich the molecular mechanism of plant-microbe interaction. Progresses have been achieved in the pathogen phylogenetic placement, the alternative hosts, the pathogen morphology and diversity, the toxins generated by false smut balls, the artificial inoculation method, and the pathogen transformation as well as rice resistance to the disease. However, it is still controversy on the infection process. It is not clear how the life cycle of this pathogen is coupled with the disease cycle. This review summarized our current understanding on the pathogen, the pathogenesis, and the rice resistance to the disease. Future work should pay attention to developing a more rapid and effective system to evaluate rice resistance and susceptibility to the disease, screening of rice germplasm for disease-resistance breeding, studying the resistance inheritance, and investigating the molecular mechanism of rice-false smut fungus interaction.

Analysis of single frequency modulation for passive hydrogen maser

Based on the theory of the passive hydrogen maser, along with the technology of frequency modulation and modulation transfer spectroscopy, the theoretical expression of the single frequency modulation for the passive hydrogen maser and the function of the cavity and H line error signals separation are derived, which are basically coincident with the experiment. The absorption and dispersion spectrum curves with different resonance widths show that the cavity and hydrogen transition serve as discriminators, and the two error signals can be separated. Through the calculations of the two error signals in the passive hydrogen maser, it analyzes the traditional method of the two error signals separation, and then describes a new improved method for the passive hydrogen servo loops consisting in the use of a single modulation frequency and frequency discrimination. A null interaction of the two error signals for the new selection of the phase setting is deduced theoretically and validated by the simulation. The preliminary experimental result confirms the feasibility of this new approach, which can reduce the influence from the cavity frequency variety on the crystal oscillator and contribute significantly to the long term performance of the passive hydrogen maser.

Responses of Seed Yield and Economic Benefit of Winter Oilseed Rape(Brassica napus L.) to Different NPK Ratio and Dose

Field experiments were conducted during 2008-2010 to investigate the ef- fects of NPK fertilization on seed yield and economic benefit of a new hybrid cultivar of winter oilseed rape （Brassica napus L.） - Xiangzayou763, and to determine the optimum NPK ratio and dose in Hunan, China. The results showed that NPK ratio （1：0.50：0.50） with 180 kg/hm2 N, 90 kg/hm2 P2O5 and 90 kg/hm2 K20 was the optimum combination for the highest seed yield （2 231.13 kg/hm2） and economic benefit （9 816.97 Yuan/hm2）, of which 1 641.23 Yuan/hm2 being set for fertilizer in- vestment （VCR=4.11）. Besides, the effects of N, P and K fertilizers on seed yield and oil production of winter oilseed rape were not identical. N had the greatest ef- fect on plant growth of winter oilseed rape, followed by P and K. Total leaf number, green leaf number, leaf length and width, rhizome diameter, branch number, dry weight per plant and other agronomic characters in high N treatments were higher than those in low N treatments. By correlation and path analysis of yield components, the contribution rate to seed yield was found： effective silique number per plant〉seed number per silique〉l 000-seed weight.

The rice miR171b–SCL6-IIs module controls blast resistance,grain yield,and flowering

Micro RNAs(mi RNAs)act as regulators of plant development and multiple stress responses.Here we demonstrate that the rice mi R171 b-SCL6-IIs module regulates the balance between blast resistance,grain yield,and flowering.mi R171 b-overexpressing rice plants(OX171 b)displayed increased rice blast resistance accompanied with enhanced defense responses and late heading,whereas blocking mi R171 b expression in rice(MIM171)led to greater susceptibility to blast disease,associated with compromised defense responses and early heading.Either overexpressing or silencing of mi R171 b significantly affected plant height and number of filled seeds per panicle(seed-setting rate),resulting in decreased grain yield.mi R171 b targets SCL6-IIa,SCL6-IIb,and SCL6-IIc,whose expression was suppressed in OX171 b but increased in MIM171.Mutants of SCL6-IIa,SCL6-IIb,and SCL6-IIc all displayed phenotypes like that of OX171 b,including markedly increased blast disease resistance,slightly decreased grain yield,and delayed flowering.Amounts of mi R171 b increased gradually in leaves during the vegetative stage but decreased gradually in panicles during the reproductive stage,whereas SCL6-IIs displayed the reverse expression pattern.Together,these results suggest that the expression of mi R171 b was time-and space-dependent during the rice growth period and regulated the balance between rice blast disease resistance,grain yield,and flowering via SCL6-IIs,and that appropriate accumulation of mi R171 b is essential for rice development.

Technology and test results of space adaptability of passive hydrogen maser

Based on the operating principle and the electric property design of the passive hydrogen maser, the technology and test results of its space adaptability are carried out under the special launch conditions and space environment. The various perturbations affecting the output frequency of such a standard used for the navigation satellite system are specified, such as magnetic field change, vibration, thermal vacuum and radiation. Through the adaptability technology in the aspects above, the security and reliability of the space passive hydrogen maser sufficiently fulfill the requirements of space operation. At present, the space passive hydrogen maser is working normally on board, indicating that the space adaptability satisfies the design requirement.

Exploration of risk factors for hemoglobinuria and acute kidney injury following iliofemoral venous mechanical thrombectomy

Objective: To evaluate the risk factors for hemoglobinuria and acute kidney injury(AKI) after percutaneous mechanical thrombectomy(MT) with or without catheter-directed thrombolysis(CDT) for iliofemoral deep vein thrombosis(IFDVT).Methods: Patients with IFDVT who had MT with the Angio Jet catheter(group A), MT plus CDT(group B), or CDT alone(group C) from January 2016 to March 2020 were retrospectively evaluated. Hemoglobinuria was monitored throughout the treatment course, and postoperative AKI was assessed by comparing the preoperative(baseline) and postoperative serum creatinine(sCr) levels from the electronic medical records of all patients. AKI was defined as an elevation in the sCr level exceeding 26.5 μmol/L within 72 h after the operation according to the Kidney Disease Improving Global Outcomes criteria.Results: A total of 493 consecutive patients with IFDVT were reviewed, of which 382(mean age, 56 ± 11 years;41% of them were females;97 in group A, 128 in group B, and 157 in group C) were finally analyzed. Macroscopic hemoglobinuria was evident in 44.89% of the patients of the MT groups(101/225, 39 in group A, and 62 in group B), with no significant difference between the groups(P = 0.219), but not in the patients in group C. None of the patients developed AKI(mean sCr difference-2.76 ± 13.80 μmol/L, range =-80.20 to 20.60 μmol/L) within 72h after surgery.Conclusions: Rheolytic MT is an independent risk factor for hemoglobinuria. A proper aspiration strategy, hydration, and alkalization following thrombectomy are particularly favorable for preventing AKI.

Experimental study on the sampling efficiency of the whirl type separation sand sampler in a wind tunnel

A performance test was conducted in a wind tunnel by changing the principal configuration parameters of a sampler such as the diameter of the container, inlet width and cone height. The results show that the average sand collection rate is from 80% to 90% when any one of the configuration parameter levels is changed. However, the variation of a parameter level results in different ef-fects on the sand collection rate for each soil sample within a certain size range of sand grains. The results show that for various sand grain sized soil sample at each wind speed, the sand collection rate decreases when the diameter of the container changes from 50 mm to 40 mm, the sand collection rate increases by about 2%-3% when the inlet width changes from 10 mm to 8 mm, and the sand collection rate increases by about 3%-4% when cone height is altered from 100 mm to 125 mm. The average sand collection rate is enhanced by 2%-4% for the soil sample of different sized sand grains when the diameter of the container is 50 mm, the inlet width is 8 mm, and cone height is 125 mm.

Efficient removal of tetracycline hydrochloride through novel Fe/BiOBr/Bi_(2)WO_(6) photocatalyst prepared by dual-strategy under visible-light irradiation

It is important to investigate whether combining two modification strategies has a synergistic effect on the activity of photocatalysts.In this manuscript,Fe-doped BiOBr/Bi_(2)WO_(6) heterojunctions were synthesized by a one-pot solvothermal method,and excellent photocatalytic performance was obtained for the degradation of tetracycline hydrochloride(TCH)in water without the addition of surfactant.Combining experiments and characterization,the synergistic effect between Fe ion doping and the BiOBr/Bi_(2)WO_(6) heterojunction was elucidated.The Fe/BiOBr/Bi_(2)WO_(6) composite photocatalyst had a beneficial void structure,enhanced visible light response,and could inhibit the recombination of photogenerated support well,which improved the photocatalytic activity.The presented experiments demonstrate that Fe/BiOBr/Bi_(2)WO_(6) removes 97% of TCH from aqueous solution,while pure BiOBr and Bi_(2)WO_(6) only remove 56% and 65% of TCH,respectively.Finally,the separation and transfer mechanisms of photoexcited carriers were determined in conjunction with the experimental results.This study provides a new direction for the design of efficient photocatalysts through the use of a dual co-modification strategy.

Transcriptional Regulation of miR528 by OsSPL9 Orchestrates Antiviral Response in Rice

Many microRNAs (miRNAs) are critical regulators of plant antiviral defense.However,little is known about how these miRNAs respond to virus invasion at the transcriptional level.We previously show that defense against Rice stripe virus (RSV) invasion entailed a reduction of miR528 accumulation in rice,alleviating miR528-mediated degradation of L-Ascorbate Oxidase (AO) mRNA and bolstering the antiviral activity of AO.Here we show that the miR528-A0 defense module is regulated by the transcription factor SPL9.SPL9 displayed high-affinity binding to specific motifs within the promoter region of miR528 and activated the expression of miR528 gene in vivo.Loss-of-function mutations in SPL9 caused a significant reduction in miR528 accumulation but a substantial increase of AO mRNA,resulting in enhanced plant resistance to RSV.Conversely,transgenic overexpression of SPL9 stimulated the expression of miR528 gene,hence lowering the level of AO mRNA and compromising rice defense against RSV.Importantly,gain in RSV susceptibility did not occur when SPL9 was overexpressed in mir528 loss-of-function mutants,or in transgenic rice expressing a miR528-resistant AO.Taken together,the finding of SPL9-mediated transcriptional activation of miR528 expression adds a new regulatory layer to the miR528-A0 antiviral defense pathway.

Insight into the Ebola virus nucleocapsid assembly mechanism： crystal structure of Ebola virus nucleoprotein core domain at 1.8 A resolution

Ebola virus （EBOV） is a key member of Filoviridae family and causes severe human infectious diseases with high morbidity and mortality. As a typical negative-sense single-stranded RNA （-ssRNA） viruses, EBOV possess a nucleocapsid protein （NP） to facilitate genomic RNA encapsidation to form viral ribonucleoprotein complex （RNP） together with genome RNA and polymerase, which plays the most essential role in virus proliferation cycle. However, the mechanism of EBOV RNP formation remains unclear. In this work, we solved the high resolution structure of core domain of EBOV NP. The polypeptide of EBOV NP core domain （NPcore） pos- sesses an N-lobe and C-lobe to clamp a RNA binding groove, presenting similarities with the structures of the other reported viral NPs encoded by the members from Mononegavirales order. Most strikingly, a hydrophobic pocket at the surface of the C-lobe is occupied by an a- helix of EBOV NPcore itself, which is highly conserved among filoviridae family. Combined with other bio- chemical and biophysical evidences, our results provides great potential for understanding the mechanism underlying EBOV RNP formation via the mobility of EBOV NP element and enables the development of antiviral therapies targeting EBOV RNP formation.

The nucleoprotein of severe fever with thrombocytopenia syndrome virus processes a stable hexameric ring to facilitate RNA encapsidation

Severe fever with thrombocytopenia syndrome virus(SFTSV),a member of the Phlebovirus genus from the Bunyaviridae family endemic to China,is the causative agent of life-threatening severe fever with thrombocyto-penia syndrome(SFTS),which features high fever and hemorrhage.Similar to other negative-sense RNA viruses,SFTSV encodes a nucleocapsid protein(NP)that is essen-tial for viral replication.NP facilitates viral RNA encapsida-tion and is responsible for the formation of ribonucleopro-tein complex.However,recent studies have indicated that NP from Phlebovirus members behaves in inhomogene-ous oligomerization states.In the present study,we report the crystal structure of SFTSV NP at 2.8Åresolution and demonstrate the mechanism by which it processes a ring-shaped hexameric form to accomplish RNA encapsida-tion.Key residues essential for oligomerization are identi-fi ed through mutational analysis and identifi ed to have a signifi cant impact on RNA binding,which suggests that correct formation of highly ordered oligomers is a criti-cal step in RNA encapsidation.The fi ndings of this work provide new insights into the discovery of new antiviral reagents for Phlebovirus infection.

Distinct structural characteristics define a new subfamily of Mycoplasma ferritin

Ferritins can generally be divided into four subfamilies based on their structural characteristics,namely,the classic ferritins(Ftns),bacterioferritins(Bfrs),DNA-binding proteins from starved cells(Dps’),and encapsulated ferritins(Enc Ftns).However,the ferritin from Mycoplasma penetrans(Mpef)possesses a particular ferroxidase center with an extreme low activity and exhibits unusual characteristics,indicating that it could be a member of a quite different subfamily of ferritins.Hereby,the crystal structure of the ferritin from Ureaplasma urealyticum(Uurf)is presented,Mpef and Uurf have very similar properties,though they display very low sequence similarity.Thus,ferritins from Mycoplasma with these unique properties do not belong to any known subfamily,but they should rather be placed in a novel ferritin subfamily,which we term Mycoplasma Ferritin(Mfr).

Role of small RNAs in the interaction between Arabidopsis and Pseudomonas syringae

Endogenous small RNAs(miRNAs and siRNAs)regulate gene expression in diverse biological processes.Research with the Arabidopsis-Pseudomonas syringae system has shown that small RNAs contribute to plant immunity by regulating the expression of their target genes.Plant immunity can be triggered by pathogen-associated molecular patterns(PAMPs)or effector proteins that are delivered into the host cell by the pathogen.Experimental evidence indicates that the miRNA pathway play a major role in PAMP-triggered immunity while some of the siRNA pathways appear to be more important in effector-triggered immunity.In addition,some P.syringae effector proteins appear to inhibit miRNA biogenesis or function to enhance bacterial virulence.These exciting findings illustrate a new battle ground for plant-pathogen interactions.